Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,516	0	C	T	100.0%	114.9 / NA	33	H33Y (CAC→TAC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/14);  total (19/14)

CCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329450‑1329588                                                                   |                                                                         ccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgcg                                                                 <  2:1114127/76‑1 (MQ=255)    gCAAAAGCCATAACGATCTACAAGTATCTGGGTTGTGACNNCGTGGTGNAATCCTGCGTCGGTTTCATCCGCGAtt                                                              <  1:1454992/76‑1 (MQ=255)    gCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAtt                                                              >  2:301791/1‑76 (MQ=255)       aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                           <  1:167565/76‑1 (MQ=255)        aaGCCAAAACGATCNACAAGTATCAGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc                                                          >  1:1622/1‑76 (MQ=255)        aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTCGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc                                                          >  2:1179735/1‑76 (MQ=255)        aaGCCAAAACGATCAACAAATATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc                                                          >  1:1255293/1‑76 (MQ=255)         aGCCAAAACGATCAACAAGTANCTNGGTAGTGACTACGTGGTGAAANCCAGCGTNGNTTACATCCGCGATTTGCCg                                                         >  2:221501/1‑76 (MQ=255)            cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      <  2:1396294/76‑1 (MQ=255)             aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                     <  2:1317476/76‑1 (MQ=255)               aaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGt                                                   >  2:763003/1‑76 (MQ=255)                      aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg                                            <  1:964663/76‑1 (MQ=255)                       acaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGc                                           >  2:89854/1‑76 (MQ=255)                         aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg                                         >  1:300019/1‑76 (MQ=255)                           gTATCGGGGTAGGGGCTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGcc                                       <  2:468881/76‑1 (MQ=255)                            tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa                                      >  1:858656/1‑76 (MQ=255)                            tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa                                      >  2:604682/1‑76 (MQ=255)                                     aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTCGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                             >  2:189233/1‑76 (MQ=255)                                            aCGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActc                      >  2:415806/1‑76 (MQ=255)                                            aCGTGGTGAAATCCAGCGTCAGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActc                      >  2:128875/1‑76 (MQ=255)                                             cGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     >  2:767191/1‑76 (MQ=255)                                              tgggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    <  2:808439/74‑1 (MQ=255)                                                 gtgAAATCCAGCGTTGGGTGCAACCGCGATTTGCCGACCAGTGGCTCAGCTCCCACAAAGAGTGCCGACTCTACCt                 <  1:72384/76‑1 (MQ=255)                                                 gtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCt                 <  1:682034/76‑1 (MQ=255)                                                  tgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTc                <  2:951744/76‑1 (MQ=255)                                                   gAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcc               >  1:1112240/1‑76 (MQ=255)                                                    aaaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTTTTCCTcca              <  1:1374249/76‑1 (MQ=255)                                                     aaTCCAGCGGCGGTTACCTCCGCGAATTGTCGACCAGTGNCTCAGGTGCCAAAAANAGTGCCGACTCTACCTccac             >  2:1475015/1‑76 (MQ=255)                                                       tCCAGCGTNGGTTACATCCGCGATTTGACGACCGGGGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacca           <  2:249580/76‑1 (MQ=255)                                                        ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          >  1:512596/1‑76 (MQ=255)                                                          aGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCCCCAAGa        >  1:990083/1‑76 (MQ=255)                                                            cGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACg      >  1:1014909/1‑76 (MQ=255)                                                                ggTTACATNCGCGATTTGCCGACCAGTGGCTCATGCGCCAAAAAGAGTGCCGTCTCTACCTCCACCAAGACGGCTa  <  2:248594/76‑1 (MQ=255)                                                                ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  2:197889/76‑1 (MQ=255)                                                                   |                                                                         CCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329450‑1329588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑