Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,500,863	G→A	A134T (GCG→ACG)	tktB →	transketolase 2, thiamin‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,500,863	0	G	A	100.0%	78.0 / NA	22	A134T (GCG→ACG)	tktB	transketolase 2, thiamin‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/14);  total (8/14)

CACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGA  >  REL606/2500796‑2500935                                                                    |                                                                         caccGGCCCGCTGTGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGACGCAGttt                                                                  <  2:1277145/76‑1 (MQ=255)  accGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTa                                                                 >  2:1303723/1‑76 (MQ=255)      gCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTAAcca                                                             <  2:754872/76‑1 (MQ=255)        ccGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTAAccagc                                                           <  1:603364/76‑1 (MQ=255)             tGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGAc                                                      <  1:382346/76‑1 (MQ=255)             tGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGAc                                                      >  2:1243783/1‑76 (MQ=255)                  aaGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATga                                                 <  1:334018/76‑1 (MQ=255)                     gTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGAt                                              <  1:275366/76‑1 (MQ=255)                       ttGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGAtcg                                            >  2:457077/1‑76 (MQ=255)                        tGGCGAACGCCGTCGGGATGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTAAACAGCCAGACCATGAGAtcgt                                           >  1:1464268/1‑76 (MQ=255)                                 ccGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACtt                                  <  2:1081824/76‑1 (MQ=255)                                       ggCTGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTa                            <  1:517280/76‑1 (MQ=255)                                        gCTGGCGATAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTAt                           <  1:661295/76‑1 (MQ=255)                                              gATAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGttt                     <  2:1013943/76‑1 (MQ=255)                                               aTAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTa                    <  2:1028532/76‑1 (MQ=255)                                               aTAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTa                    <  2:1110335/76‑1 (MQ=255)                                                    agagCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGc               >  2:42304/1‑76 (MQ=255)                                                    agagCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGc               <  1:297693/76‑1 (MQ=255)                                                     gagCGGACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATCTGTTTATGGGCg              <  2:58730/76‑1 (MQ=38)                                                        cGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACg           >  1:842146/1‑76 (MQ=255)                                                            cacTGGCGACGCAGTTTAGCCNGCNAGACCATGAGATCGTCGATCANTTCACCTNTNTGTTTATGGGCGACGGCTg       >  2:210739/1‑76 (MQ=37)                                                             acTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGc      >  1:1031599/1‑76 (MQ=255)                                                                ggcgacGCAGTTTAACCCGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGCTTATGGGCGACGGCTGCCTg   >  1:1468134/1‑76 (MQ=38)                                                                 gcgacgTAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGa  >  2:819327/1‑76 (MQ=255)                                                                    |                                                                         CACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGA  >  REL606/2500796‑2500935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑