| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,376,348 | 0 | T | G | 43.2% | 208.8 / 61.4 | 155 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| Reads supporting (aligned to +/- strand): ref base T (21/67); new base G (66/1); total (87/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-23 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 208 total aligned reads displayed.
CACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376274‑2376423 | ccccAGATGAGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑ggtg < 1:6956189/74‑1 (MQ=255)cACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑ggtg < 1:5889304/76‑1 (MQ=255) cccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑ggtgg < 2:2133745/75‑1 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑ggtggt < 1:4329467/76‑1 (MQ=255) aGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑c > 2:4498327/1‑76 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑GCtt > 1:2101892/1‑76 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCtt > 1:7303249/1‑76 (MQ=255) gAGGGGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCttt < 2:338836/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑GCttt > 1:2916736/1‑76 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCctt > 1:1651327/1‑76 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCtttt > 2:4326223/1‑75 (MQ=255) cGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGGCa > 1:6548906/1‑76 (MQ=255) cGAACGGTAGCCGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCa < 2:3476829/76‑1 (MQ=255) gAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGGG‑CCTTTGccct > 2:4403836/1‑74 (MQ=255) accTGTAGCCGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCAtt < 1:4738121/74‑1 (MQ=255) accTGTAGACGCCCTCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCAtt < 1:3169128/74‑1 (MQ=255) aaCTGTAGACGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCAtt < 1:2972861/76‑1 (MQ=255) aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCAtt < 1:4626140/76‑1 (MQ=255) aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCCTTGCCAtt > 1:2557303/1‑76 (MQ=255) aCTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTc < 1:2160850/76‑1 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGG‑CCTTTGccttc > 1:4455324/1‑73 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑GCTTTGCCATTc > 2:640847/1‑76 (MQ=255) cTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGTCATTCg > 1:2784590/1‑76 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑GCTTTGTCATTCgg > 1:1984877/1‑75 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑GCTTTGGCATTCGt > 2:372071/1‑76 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCCTTGCCATTCGt > 2:2033881/1‑76 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTG‑CCTTTTTCATTCCTa > 2:6946737/1‑76 (MQ=255) tAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTAc < 2:6000589/76‑1 (MQ=255) tAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTAc < 1:7749338/76‑1 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTAc < 1:320358/76‑1 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTG‑GCCTTGCCATTCGTAc > 1:567085/1‑76 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTG‑CCCTTGGCATTCGTAcc > 1:7655575/1‑76 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGGCATTCGTAcc > 1:5582644/1‑76 (MQ=255) gACGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTAccc < 1:3055365/76‑1 (MQ=255) ccGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCg < 1:530734/75‑1 (MQ=255) cGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGc > 2:4621813/1‑76 (MQ=255) cccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCCTTTCCCTTTCG‑ACCCggca > 1:1540626/1‑73 (MQ=255) ccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTTCCATTCGTACCCGCAAc > 1:6719585/1‑76 (MQ=255) cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACg > 1:1169732/1‑76 (MQ=255) aCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGGG‑CCCTTGGCATTCTTACCCGCAAAgg > 1:1135993/1‑76 (MQ=255) cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGCCATTCGTACCCGCAACGGt > 1:7345812/1‑76 (MQ=255) tttGTCCCCGGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTa < 1:2406805/76‑1 (MQ=255) tttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTG‑CCTTTGCCATTCGTACCCGCAACGGTa > 1:5835072/1‑76 (MQ=255) tGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTAGGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTaaa < 2:7605624/76‑1 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCCTACCCGCCACGGTaaa > 1:3417369/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGCCATTCGTACCCCCCACGGTgaa > 2:4163846/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCCTTGCCATTCGTACCCGCAACGGGaaa > 1:7281653/1‑76 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGCCATTCGTACCCGCAACGGGaaaaa > 2:3669714/1‑74 (MQ=255) ccccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAacac < 2:1325623/75‑1 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAacac > 1:3611314/1‑76 (MQ=255) cccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAAcaca < 1:2025293/76‑1 (MQ=255) cccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGGG‑ACCTTGTTATTCCTGCCCGCAACGGGAAAcaca > 1:431852/1‑76 (MQ=255) cccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTG‑CTTTTGCCATTCGTACCCGCAACGGTAAAcaca < 2:3189176/76‑1 (MQ=255) ccggccgccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACaa < 1:5433383/73‑1 (MQ=255) ccTCCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACaa < 2:6095936/76‑1 (MQ=255) cTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGCCATTCCTTTCCGCAACGGGGAACACaaa > 2:3654160/1‑76 (MQ=255) tGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTG‑CCTTTGCCATTCGTACCCGCCACGGGAAACACAAAt > 1:3238699/1‑76 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGGG‑GCTTTGCCATTCGTAACCGCCACGGTAAACACaaaag > 2:2658570/1‑74 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑GCTTTGGCATTCGTACCCGCAACGGGAAACACCAATg > 1:1531922/1‑76 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGCCATTCGTACCCGCCACCGGAAACACaacag > 1:1005304/1‑73 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACaaaag > 1:6361411/1‑74 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATg > 1:939265/1‑76 (MQ=255) ccgccATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCCTTGCCATTCGTACCCGCCACGGTAAACACAAATgg > 1:293332/1‑76 (MQ=255) gccATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTTCCATTTGTACCCGCAACGGGAAACACAAATGgcg > 1:7417262/1‑76 (MQ=255) cATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCt > 1:5639573/1‑76 (MQ=255) tCAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGCCATTCGTACCCGCAACGGGAAACACAAATGGCGCTgg > 2:2907630/1‑76 (MQ=255) cAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTTTCATTCGTACCCCCAAAGGTAAACACAAAAGGGCCTGGt > 2:4780451/1‑76 (MQ=255) cAGAATCGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGCCATTCGTACCCGCAACGGTAAAAACAAATGGGGCTGGt > 1:6493132/1‑76 (MQ=255) gAATCGCCTCCAGCGTGCGGCA‑GGTGGTG‑CCTTTCCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt < 1:483054/76‑1 (MQ=255) aaccGACTCCAGCGTACGGCA‑GGGGGTG‑CCTTTGCCATTCGTGCCCGCAACGGTAAACACAAATGGCGCTGGTTTc < 1:4835532/73‑1 (MQ=255) aaTCGCCTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTc < 2:54954/76‑1 (MQ=255) aTCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCa < 1:3362790/76‑1 (MQ=255) aTCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCa < 2:5392482/76‑1 (MQ=255) aTCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCa < 1:373182/76‑1 (MQ=255) aTCGACTCCAGCGTACGGCA‑GGGGGTG‑GCTTTTGCATTCGTACCCGCCACGGGAAACACCAATGGGGCTGGgttta > 2:5613383/1‑74 (MQ=255) tCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAg < 1:1048851/76‑1 (MQ=255) cGACTCCCGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 2:5472752/76‑1 (MQ=255) cGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 1:4173985/76‑1 (MQ=255) cGACTCCAGCGTACGGCA‑GGGGGGG‑CCTTTGACATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg > 2:718752/1‑76 (MQ=255) gccTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGa < 2:3934265/74‑1 (MQ=255) gACTCCAGCGTACGGCA‑GGGGGTG‑CCTTTGCCATTCGTACCCGCAACGGCAAACAAAAATTGCGCTGGTTTCACGa > 1:6995285/1‑76 (MQ=255) tCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGc < 2:5708434/76‑1 (MQ=255) cAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCa < 2:6696613/76‑1 (MQ=255) cAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCa < 2:1103833/76‑1 (MQ=255) aGCGTACGGCA‑GGTGGTG‑CCTTTTCCATTCGTACCCGCAACGGTAAATACAAATCGCGCTGGTGTCATGACCCCaa > 2:1790228/1‑76 (MQ=255) gCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 1:5767302/76‑1 (MQ=255) gCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 1:1353631/76‑1 (MQ=255) gTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAc < 1:7480741/76‑1 (MQ=255) gTACGGCA‑GGGGGTG‑CCTTTGCCATTCGTACCCGCCACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAc > 2:235864/1‑76 (MQ=255) tggCA‑GGTGGTG‑CCTTGTCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg < 1:5126111/75‑1 (MQ=255) cGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg < 2:2965331/76‑1 (MQ=255) gCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgcc > 1:7193717/1‑76 (MQ=255) gCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgcc > 2:1499137/1‑76 (MQ=255) cA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg > 2:1653241/1‑76 (MQ=255) cA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg > 1:3497644/1‑76 (MQ=255) cA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg > 1:6026387/1‑76 (MQ=255) cA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg > 1:6164718/1‑76 (MQ=255) a‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 1:4804138/1‑76 (MQ=255) gtggtg‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa > 1:2223223/1‑76 (MQ=255) gtggtg‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa > 1:1630435/1‑76 (MQ=255) | CACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTG‑CCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376274‑2376423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |