| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,378,885 | 0 | T | G | 41.1% | 352.3 / 88.9 | 209 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
| Reads supporting (aligned to +/- strand): ref base T (31/92); new base G (86/0); total (117/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.67e-32 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 282 total aligned reads displayed.
GCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378810‑3378960 | gCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgt < 2:868193/76‑1 (MQ=255)gCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgt < 2:6234009/76‑1 (MQ=255)gCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgt < 1:6419465/76‑1 (MQ=255)gCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgt < 1:6802707/76‑1 (MQ=255) tGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgtgt < 2:478153/76‑1 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgtggt > 1:1708016/1‑74 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGtt < 2:6878344/76‑1 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGtt < 1:2356239/76‑1 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGtt > 1:1925032/1‑76 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGtt > 2:3372457/1‑76 (MQ=255) ccGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTAc < 1:5307248/76‑1 (MQ=255) cGCCTTTACCTTGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTAcc > 1:6702633/1‑76 (MQ=255) gCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCt < 1:7308140/76‑1 (MQ=255) gCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCt > 1:2594644/1‑76 (MQ=255) ccTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTg < 1:3167685/76‑1 (MQ=255) ccTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTg > 2:4334911/1‑76 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTgg > 1:4159065/1‑76 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGTGTGTTACCTGGt > 1:3272253/1‑76 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGt < 2:3978948/76‑1 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGt > 1:3645699/1‑76 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGt > 1:5807264/1‑76 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGt > 1:4791424/1‑76 (MQ=255) ttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTgggt > 1:893787/1‑74 (MQ=255) tACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTGCCTGGttt > 1:3350922/1‑76 (MQ=255) tACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGttt > 1:1113872/1‑76 (MQ=255) tccTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTg < 1:5437906/75‑1 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTg < 1:4759877/76‑1 (MQ=255) gTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgc > 2:5035868/1‑76 (MQ=255) gTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgc < 2:361810/76‑1 (MQ=255) gTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgc > 2:3829412/1‑76 (MQ=255) gTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTTACTGGTTTGATTgc > 1:1770762/1‑76 (MQ=255) ttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcag < 1:5575001/76‑1 (MQ=255) ttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTgccg > 1:7606742/1‑74 (MQ=255) tCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCCGGGTTGGTTgtagc > 1:5074296/1‑76 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcagca < 1:5221093/76‑1 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcagca < 2:454063/76‑1 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcagca < 2:572849/76‑1 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTTGTTgcagca > 2:5443809/1‑76 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTgtagca > 1:3228854/1‑76 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTgcagca > 1:4921101/1‑76 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGGTTGATTgcagca > 1:4684748/1‑76 (MQ=255) tttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATc < 2:541651/76‑1 (MQ=255) ttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTTGTTGCAGCAtta > 1:5918492/1‑74 (MQ=255) ttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATCa > 1:824317/1‑76 (MQ=255) tACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGGTTGATTGCAGGCTCAACg > 1:4517576/1‑76 (MQ=255) aCGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTTATTGGAGCATCAACGc > 2:6322927/1‑76 (MQ=255) cGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGcc > 2:1516067/1‑76 (MQ=255) cGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCCGGGTTGATTGCAGCATTAAAGcc > 1:1455743/1‑76 (MQ=255) gTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCa < 2:7529966/76‑1 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTTGTTTGGTTTCAGGATCCAAGCCAg > 2:7355576/1‑76 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAAACCCAg > 1:7633524/1‑76 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCCGCATCAACGcccg > 1:7150848/1‑74 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTTATTGCAGGATCAACGCCCGAc > 1:2506475/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCacac > 2:302901/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCCGAc > 1:4399442/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGAc > 1:1646221/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGAc > 2:267478/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGAc > 2:792411/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATCAACGCCCGAc > 2:3944028/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGGTTGATTGCAGCATCCACGCCAGAc > 1:3974459/1‑76 (MQ=255) aaCAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGa > 2:1629200/1‑76 (MQ=255) aaCAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGa > 2:4651984/1‑76 (MQ=255) aGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATc < 1:2601223/76‑1 (MQ=255) aGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATc < 1:3498243/76‑1 (MQ=255) aGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATc < 1:1282564/76‑1 (MQ=255) tAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTg < 1:7501625/76‑1 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTgg > 1:5159283/1‑76 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTgg > 2:5219228/1‑76 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTgg > 2:5776536/1‑76 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATCAACGCCAGACTGAATCTgg > 2:6078338/1‑76 (MQ=255) aaccaaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTc < 1:2871816/76‑1 (MQ=255) aaccaaccGGGATGTTGCGCAGCGGCAGCGTGTTCCCTGGTTTGATTGCAGCATCAACGCCAGACTGATACTGGTc < 2:6655072/76‑1 (MQ=255) accaaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCg < 2:1925032/76‑1 (MQ=255) accaaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCg < 2:589220/76‑1 (MQ=255) ccaaccGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGc > 1:4050820/1‑76 (MQ=255) ccaaccGGGATGTTGCGCATCGGCAGGGGGTTACCTCGTTTTATTGCAGCATCAAAGCCAGACTGAATCTGGtgcc > 1:465297/1‑73 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGcc < 2:1265320/76‑1 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGcc < 1:5977276/76‑1 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGcc < 2:3002738/76‑1 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGGCGcc > 2:4040241/1‑76 (MQ=255) cGGGATGTTGCGCATCGGCAGGGGGGTACCTGGTTTGATTGCAGCATCAACGCCCGACTGAATCTGGTCGCCAGCt > 2:4919704/1‑76 (MQ=255) gggATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCtt > 1:1967350/1‑76 (MQ=255) gATGTTGCGCATCGGCAGTGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTc < 2:7687528/76‑1 (MQ=255) aTGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCa > 1:2130144/1‑76 (MQ=255) tGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAg < 2:4092322/76‑1 (MQ=255) gTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAgg < 2:683584/76‑1 (MQ=255) ttGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGc < 2:1208659/76‑1 (MQ=255) ttGCGCATCGGCAGGGGGTTACCTGGTTTGATTGAAGCATCAACGCCAGACTTAATCTGGTCGCCAGCTTTCAGGc > 2:5126647/1‑76 (MQ=255) cgcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCtt < 1:6057459/76‑1 (MQ=255) cgcATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGGCtt > 2:321277/1‑76 (MQ=255) cATCGGCAGGGGGGTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCCGCTTTCAGGCCtttt > 2:2486348/1‑75 (MQ=255) aTCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAg < 1:5123982/76‑1 (MQ=255) tCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAgg < 2:103216/76‑1 (MQ=255) cGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAggg > 1:3732754/1‑76 (MQ=255) gCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGc > 1:5509140/1‑76 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc < 1:3809534/76‑1 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 2:2196711/1‑76 (MQ=255) cAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 2:5675115/1‑76 (MQ=255) gggTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAg < 2:5271638/76‑1 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTAGCCAGCTTTCAGGTCTTTAGGGGCCAGGAt > 2:1811488/1‑76 (MQ=255) | GCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378810‑3378960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |