| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,774,914 | 0 | C | G | 41.6% | 253.2 / 196.7 | 198 | A20P (GCC→CCC) | yicJ | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (4/111); new base G (82/0); total (86/112) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 251 total aligned reads displayed.
CGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATGCT > REL606/3774839‑3774989 | cGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGtggctggc < 2:7174780/76‑1 (MQ=255) ggCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggcg < 2:7616388/76‑1 (MQ=255) gCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggcgg < 1:1435303/76‑1 (MQ=255) cAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTgggggg > 2:1302243/1‑75 (MQ=255) cAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggcggc < 1:2313879/76‑1 (MQ=255) tGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑cc < 2:2055451/76‑1 (MQ=255) gCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑ccc < 2:6490429/76‑1 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCa < 2:6204770/76‑1 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCa < 2:27187/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCAt < 1:7672262/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCAt < 1:972041/76‑1 (MQ=255) atatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAt < 2:769517/76‑1 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑a < 1:293586/76‑1 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑a < 1:5126088/76‑1 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑a < 1:6681679/76‑1 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑a < 1:4147816/76‑1 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑GT‑CCCCTGCCAC‑aa > 1:3422907/1‑76 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑aa < 2:5938321/76‑1 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑aa < 1:6972887/76‑1 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑aa < 1:5609178/76‑1 (MQ=255) tCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑c < 2:56687/76‑1 (MQ=255) cGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCCT‑AA‑c‑c > 2:6979963/1‑76 (MQ=255) ggTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑Ca < 1:2812246/76‑1 (MQ=255) gTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CC‑CCCCTGCCCT‑AA‑C‑Caa > 1:1034239/1‑76 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCATGCCAT‑AA‑A‑CAAt > 1:6518069/1‑76 (MQ=255) ataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCCT‑AA‑C‑CCAtt > 1:4288090/1‑76 (MQ=255) ataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCCT‑AA‑C‑CAAtt > 2:286841/1‑76 (MQ=255) ataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGCGG‑GT‑CCCCTGCCCT‑AA‑C‑Caaat > 1:998279/1‑74 (MQ=255) taAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCCT‑AA‑A‑CAAatt > 1:3187305/1‑76 (MQ=255) taAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAAttt < 1:5876946/76‑1 (MQ=255) aaaaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAAtttt < 1:5407492/76‑1 (MQ=255) aaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CG‑CCCCTGCCCT‑AA‑A‑CAAattttt > 1:5736407/1‑74 (MQ=255) aGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑GC‑CCCCTGGCCT‑AA‑C‑CAAatttttt > 2:665127/1‑73 (MQ=255) gAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTctct < 2:7307878/76‑1 (MQ=255) aaCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCCC‑AA‑C‑CCATTTTTTCtt > 2:5202856/1‑76 (MQ=255) aaCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCtt < 1:6261100/76‑1 (MQ=255) aCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCttt < 1:2294152/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTa < 2:860752/76‑1 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGT‑GT‑CCCTATCCCT‑AA‑C‑CACATTTCTCttttt > 1:3634171/1‑74 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGT‑CT‑TCCCTGCGCT‑AA‑C‑CAAttttttttttca > 1:4562439/1‑74 (MQ=255) tcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAAc < 1:4749664/76‑1 (MQ=255) catATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACg < 1:2199463/76‑1 (MQ=255) catATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACg < 1:1238309/76‑1 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTTCCCTAAA‑C‑CAATTTTTTTTTTatgg > 2:3165185/1‑73 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCAT‑AA‑CACAATTTT‑TCTTTTAcag > 2:2372396/1‑74 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCAT‑AA‑C‑CAAATTTCTCTTTTAccg > 1:1578363/1‑74 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGCGGGGC‑GT‑CCCCCCCCAT‑AA‑C‑CAAATTTTTCTTTTAcag > 2:1963748/1‑74 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACgg < 1:1791535/76‑1 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACgg < 2:1755064/76‑1 (MQ=255) tatataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGa < 1:531308/76‑1 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CTCCCCTTCCCAC‑AC‑C‑CAAATTTCTTTTTAACgtc > 1:7429198/1‑74 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCATGCCCT‑AA‑C‑CAAATTTTTTTTTTATGGac > 1:6174324/1‑76 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGCGTGCT‑CCC‑CGCCAT‑AACC‑CAATTTCCTCTTAAACgac > 1:3871027/1‑74 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGaca < 2:3767857/76‑1 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGaca < 1:6900167/76‑1 (MQ=255) ataACATTACGTTATCGAAAATAATGTGGCTGGGGGCGG‑CT‑CCCCTGCCAT‑AA‑C‑CAAATTTTTTTTTATCGGacaa > 1:3466597/1‑76 (MQ=255) aaCATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCAT‑AA‑A‑CAAATTTTTTTTTTACGGacaaaa > 1:3154824/1‑74 (MQ=255) cATTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCCGCCAT‑AA‑C‑CAATTTTTTCTTTTACGGACAacaca > 2:2407750/1‑75 (MQ=255) cATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACt < 1:7214393/76‑1 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCCTCCCT‑AA‑C‑CCATTTTTTCTTTTACGGAAAACACtt > 2:6394762/1‑76 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGGGGCGG‑CT‑CCCCTGCCAT‑AA‑A‑CAAATTTCTCTTTTACAGAAAAAACtt > 1:793351/1‑76 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGGGGCGG‑CT‑CCCCCGCCAC‑AA‑C‑CAAATTTTTCTTTTACCGGCAAAACtt > 1:155098/1‑76 (MQ=255) ttACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTc < 2:4823894/76‑1 (MQ=255) ttACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTc < 2:1585665/76‑1 (MQ=255) aCGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTTTCTTTTACGGAAAAAACTTccc > 1:835224/1‑74 (MQ=255) cGTTATCGAAAATAATGTGGCTGGGGGGGG‑GT‑CCCCCGCCAT‑AA‑A‑CAAATTTCTCTTTTACCGGCACAACTTctct > 2:7807803/1‑76 (MQ=255) cGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCCT‑AA‑C‑CAATTTTCTCTTTTAAGGACAACCATTCAct > 1:1290624/1‑76 (MQ=255) cGTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTCCCCT‑AA‑C‑CAATTTTTTCTTTTAATGACAACAATTCAct > 2:2032185/1‑76 (MQ=255) cGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCAct < 1:2087594/76‑1 (MQ=255) gTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCCT‑AA‑C‑CAATTTTTTCTTTTACCGACAACACCTCActc > 1:6462266/1‑76 (MQ=255) gTTATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCCT‑AA‑A‑CAATTTTTTCTTTTACGGGCAAAACTTCActc > 1:2458872/1‑76 (MQ=255) ttATCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCGT‑AA‑A‑CAAATTTCTCTTTTACGGACAACACATCActct > 2:1318848/1‑76 (MQ=255) aTCGAAAATAATGTGGCTGGGGGGGG‑CT‑CCCCTGCCCT‑AA‑C‑CAATTTTCTCTTTAACGGACCAAACCTCACCCTTc > 1:6357000/1‑76 (MQ=255) aaaTAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTg < 1:5537320/76‑1 (MQ=255) aataatGTGGCTGGGGGGGG‑CT‑CCCCTGCCCT‑AA‑C‑CAAATTTTTCTTTTACGGACAACACTTCACTCTTCTTCTGc > 2:2218757/1‑76 (MQ=255) aataatGTGGCTGGCGGCTT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGc < 1:7533199/76‑1 (MQ=255) aataatGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGc < 1:1954198/76‑1 (MQ=255) ataatGTGGCTGGGGGGGG‑CT‑CCCCTGCCCT‑AA‑C‑CCAATTTTTTTTTTACCGACAAAACTTCACTCTTCAtcttct > 2:7123819/1‑76 (MQ=255) taatGTGGCTGGGGGGGG‑CT‑CCCCTCCCCT‑TA‑C‑CAAATTTTTCTTTTACGGAAAACACTTCCACCCTCATcttctt > 2:4353627/1‑76 (MQ=255) aatGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCttt < 2:420263/76‑1 (MQ=255) atGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 1:6094101/76‑1 (MQ=255) atGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 2:4440884/76‑1 (MQ=255) atGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 1:5243282/76‑1 (MQ=255) atGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 2:3461867/76‑1 (MQ=255) atGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 2:1645871/76‑1 (MQ=255) atGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 2:2306430/76‑1 (MQ=255) tggctggcGGGGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCCTTTCCt > 2:4061995/1‑76 (MQ=255) tggctggcGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCt > 1:7487668/1‑76 (MQ=255) tggctggcGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCt > 2:6583801/1‑76 (MQ=255) ggctggcGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCtt < 1:5991105/76‑1 (MQ=255) ggctggcGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTACCtt < 2:7522896/76‑1 (MQ=255) ctggcGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGa > 2:2680393/1‑76 (MQ=255) ctggcGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGa > 2:7207620/1‑76 (MQ=255) ctggcGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGa < 1:4405958/76‑1 (MQ=255) ctggcGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGa > 2:4492721/1‑76 (MQ=255) tggcGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGAt > 2:3551392/1‑76 (MQ=255) tggcGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGAt > 2:5449967/1‑76 (MQ=255) ggcggcGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATg > 1:593108/1‑76 (MQ=255) cggcgT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATGCt < 2:4619171/76‑1 (MQ=255) cggcgT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATGCt < 1:3744576/76‑1 (MQ=255) | CGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGT‑CT‑CCCATGCCAT‑AA‑C‑CAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATGCT > REL606/3774839‑3774989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |