| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 476,746 | 0 | T | G | 45.3% | 139.1 / 23.8 | 95 | Q6H (CAA→CAC) | fsr | predicted fosmidomycin efflux system |
| Reads supporting (aligned to +/- strand): ref base T (26/26); new base G (43/0); total (69/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.53e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 150 total aligned reads displayed.
TGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTAC > REL606/476673‑476821 | tGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGggtg > 1:6060786/1‑76 (MQ=255) gATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGggtgt > 1:4196619/1‑76 (MQ=255) gATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGggtgt > 2:3986162/1‑76 (MQ=255) aTAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGgttgtt > 2:3776029/1‑76 (MQ=255) cACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCTGCGCCCGCCACAGGCTGGGGGTGTTTACt > 2:4297638/1‑76 (MQ=255) aCCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTc > 2:3812563/1‑76 (MQ=255) tAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCAtt > 1:4156758/1‑76 (MQ=255) tAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCAtt > 2:2348682/1‑76 (MQ=255) aaaaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCCTTg > 2:2742891/1‑76 (MQ=255) aaaaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCCCTCATTg > 2:7677881/1‑76 (MQ=255) aaaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCCTTGc > 1:969671/1‑76 (MQ=255) aaaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCAtttc > 2:1456464/1‑74 (MQ=255) aaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGGTTAATCAtttgc > 2:5005267/1‑73 (MQ=255) aTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCa > 2:5027477/1‑76 (MQ=255) ccAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTCCTCCATGCCCTAg > 1:2238353/1‑76 (MQ=255) ccAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAg > 1:974811/1‑76 (MQ=255) ccAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAg > 1:5175616/1‑76 (MQ=255) ccAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAg > 2:5813412/1‑76 (MQ=255) cAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAgg > 2:160260/1‑76 (MQ=255) cAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAgg > 2:1207586/1‑76 (MQ=255) aaaCGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTGCCATAGGa > 1:75694/1‑76 (MQ=255) aaaCGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCAATCCTTGCCATAGGa > 2:7601575/1‑76 (MQ=255) aaCGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTGCCACATTTCTATAGGaa > 1:1974877/1‑76 (MQ=255) aaCGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAggga > 1:1702581/1‑74 (MQ=255) cGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTAATCATTGCCATAGGGAAg > 1:4440140/1‑76 (MQ=255) cGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCCTTGCCCTAGGAAAg > 2:1273850/1‑76 (MQ=255) gATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGt > 2:7241182/1‑76 (MQ=255) gATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGGTCACTCATTGTCGTGGGAAAgg > 1:5132331/1‑75 (MQ=255) tGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGGAAGGCa > 2:3725093/1‑76 (MQ=255) gTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGGCAc > 1:2918792/1‑76 (MQ=255) ttCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCCTAGGGAAGTCACt > 2:5989558/1‑76 (MQ=255) ttCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGGTCACTCATTGCCCTAGGAAAGTCACt > 1:1395575/1‑76 (MQ=255) cGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTGCCATAGGAAAGTGCCttt > 1:5163287/1‑76 (MQ=255) cGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGGCACttt > 2:4755585/1‑76 (MQ=255) gggCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACGCAATTCCATAGGAAAAACTCtttt > 2:6751574/1‑76 (MQ=255) ggCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACttttt > 2:7677013/1‑76 (MQ=255) gCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCCCTTTTTc > 1:7797439/1‑76 (MQ=255) ccTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGGTCACTTATTGCCCTAGGAAAGTCCGCTTTTCa > 1:2614214/1‑76 (MQ=255) ccTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCa > 1:3810548/1‑76 (MQ=255) cTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCCTAGGAAAGTCACTTTTTCAg > 1:7156548/1‑76 (MQ=255) ttGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGGAAGTCACTTTTTCAgg > 1:3623819/1‑76 (MQ=255) ttGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTGCCCTAGGAAAGTCACTTTTTCAgg > 1:2681984/1‑76 (MQ=255) ttGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGGAAGTCACTTTTTTAgg > 1:1999908/1‑76 (MQ=255) ttGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAgg > 1:875302/1‑76 (MQ=255) tGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGGCATAGGGAAGTCACTTTTTCAggg > 1:271031/1‑76 (MQ=255) tGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAggg > 2:5333951/1‑76 (MQ=255) ggTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGt > 1:3293517/1‑76 (MQ=255) gTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCCCTCATTGCCATAGGAAAGTCCCTTTTTCAggggt > 1:5072210/1‑74 (MQ=255) tCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCCTAGGAAAGTCACTTTTTCAGGGTTg > 1:4742966/1‑76 (MQ=255) gTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGGCATAGGGAAGTCACTTTTTCATGGGTGCg > 1:573657/1‑76 (MQ=255) gTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCCTTGCCATAGGAAAGTCACTTTTTCAGGGTTGCg > 2:3069439/1‑76 (MQ=255) gTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCg > 2:7451637/1‑76 (MQ=255) gTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTTAGGGGTGCg > 1:7310159/1‑76 (MQ=255) ttGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGGTGCGa > 2:3961280/1‑76 (MQ=255) ttGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGGTGCGa > 1:7101726/1‑76 (MQ=255) tGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTGCCATAGGAAAGTCACTTTTTCAGGGGTGCggt > 2:4756416/1‑74 (MQ=255) aCGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGt > 1:5311132/1‑76 (MQ=255) cAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTaaa > 1:4008223/1‑76 (MQ=255) ccccGCCCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAg < 1:4320035/74‑1 (MQ=255) ccGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGGTGCGATGTAAAGaa > 2:1712569/1‑76 (MQ=255) ccGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAgga > 1:3049381/1‑74 (MQ=255) ccGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGaa > 2:7001833/1‑76 (MQ=255) ccGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTACCATAGGGAAGTCACTTTTTCAGGGGTGCGATGTAAAgga > 2:351629/1‑74 (MQ=255) ccGCCCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGaa < 1:7434293/76‑1 (MQ=255) gcgcCCGCCACAGGCTGGGGGTGTTCACTCATTGCCCTAGGAAAGTCACTTTTTCAGGGTTGGGATGTAAAGAATg > 1:6567283/1‑76 (MQ=255) ccccccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATg < 1:2195958/73‑1 (MQ=255) cgcccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGa > 1:155697/1‑76 (MQ=255) cgcccgccACAGGCTGGGGGTGTTTACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTGAAGAATGa > 2:1012849/1‑76 (MQ=255) cgcccgccACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGa > 2:6396720/1‑76 (MQ=255) cgcccgccACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGa > 2:5516638/1‑76 (MQ=255) cccccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGa < 2:5323580/74‑1 (MQ=255) cccccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGa < 1:3387711/74‑1 (MQ=255) cccgccACAGGCTGGGGTTGTTTACTCTTTGGCATAGGAAAGGCACCTTTTCACGGTTGCGGTGGAAAAAATGATc > 2:1167161/1‑76 (MQ=255) cccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATc > 2:6477188/1‑76 (MQ=255) cccgccACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGAtt > 2:7094818/1‑75 (MQ=255) ccgccACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCt > 2:4652910/1‑76 (MQ=255) ccgccACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAAAATGATCt > 2:2713997/1‑76 (MQ=255) cgccACAGGCTGGGGTTGTTCACTCCTTGCCCTAGGAAAGTCACTTTTTTAGGGTTGCGATGTAAAGAATGATCtt > 1:1113837/1‑76 (MQ=255) cgccACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCtt > 2:3677708/1‑76 (MQ=255) gccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTa < 2:2946787/76‑1 (MQ=255) gccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTa < 2:2028383/76‑1 (MQ=255) gccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTa < 1:7004296/76‑1 (MQ=255) acaGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTAttt < 1:384093/76‑1 (MQ=255) caGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtg > 2:674084/1‑76 (MQ=255) caGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtg < 1:7178242/76‑1 (MQ=255) aGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtgt > 2:3373431/1‑76 (MQ=255) ggCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtgtg < 1:6189226/76‑1 (MQ=255) ggCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtgtg < 1:6656719/76‑1 (MQ=255) gCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGa < 2:6830907/76‑1 (MQ=255) cTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGat > 2:1067734/1‑76 (MQ=255) tGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatt > 1:5773688/1‑76 (MQ=255) tGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatt < 1:6296758/76‑1 (MQ=255) tGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatt < 1:822236/76‑1 (MQ=255) ggggTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatta > 2:6974428/1‑76 (MQ=255) ggggTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatta > 1:5312669/1‑76 (MQ=255) ggggTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatta > 2:3296360/1‑76 (MQ=255) gggTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGattat < 2:4528175/76‑1 (MQ=255) ggTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGattatt < 1:6437664/76‑1 (MQ=255) ttgttCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTAc > 2:6881878/1‑76 (MQ=255) ttgttCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTAc < 2:5800897/76‑1 (MQ=255) | TGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTAC > REL606/476673‑476821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |