| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 691,852 | 0 | T | G | 36.9% | 272.2 / 26.6 | 160 | intergenic (+17/‑33) | ybfM/ybfN | predicted outer membrane porin/predicted lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (55/46); new base G (59/0); total (114/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 238 total aligned reads displayed.
TCTTCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCTGG > REL606/691777‑691927 | tCTTCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGt > 1:7401313/1‑76 (MQ=255)tCTTCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGt > 1:415080/1‑76 (MQ=255) cTTCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGtt < 1:1108183/76‑1 (MQ=255) cTTCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGtt < 1:751981/76‑1 (MQ=255) ttCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGgtt > 2:4962807/1‑76 (MQ=255) tCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGtttt > 2:3685805/1‑76 (MQ=255) ccAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTtg < 1:2132854/76‑1 (MQ=255) ccAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTtg > 1:4932255/1‑76 (MQ=255) cAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTtgg > 1:4276870/1‑75 (MQ=255) aGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTtgtg > 2:7717692/1‑76 (MQ=255) ggATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGtgc < 1:3303656/76‑1 (MQ=255) aTGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTtgtgtcc > 1:2426217/1‑73 (MQ=255) aTGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGtgcct < 1:2211527/76‑1 (MQ=255) aTGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGtgcct > 1:1659094/1‑76 (MQ=255) aTGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGtgcct > 2:1216268/1‑76 (MQ=255) tGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGtgcctg < 2:2464246/76‑1 (MQ=255) gAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGtgcctg‑c > 1:1441686/1‑76 (MQ=255) gggTGACATAAATTTTATGGTAATCGCACCATTCACCATCTTCTGCTGCCCGACGCGGCAGATTTTGTCCCTG‑CCg < 2:6147529/74‑1 (MQ=255) gCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCg < 2:4806412/76‑1 (MQ=255) gCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCg < 2:2079157/76‑1 (MQ=255) gggACGTAAAATTTATGGTAATCGCCCCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTCCCTG‑CCGCa < 2:5900840/74‑1 (MQ=255) gTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTG‑CCGcc > 1:2802548/1‑75 (MQ=255) tGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCAc < 2:7041939/76‑1 (MQ=255) tGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCAc > 2:1436962/1‑76 (MQ=255) tGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCAc < 1:5638259/76‑1 (MQ=255) tGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCAc < 1:5742669/76‑1 (MQ=255) gACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACg > 1:3737320/1‑76 (MQ=255) gACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACg > 1:1773503/1‑76 (MQ=255) gTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTT‑CCGCACGttt > 2:3338724/1‑76 (MQ=255) tAAATTTTATGGTAATCGCCCCATTCCCCATCTTCTGACGCCGGCCGCGGCGGGTTTTGTCCCTG‑CCGCACGTTTg < 2:4182978/76‑1 (MQ=255) tAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTg < 2:7334489/76‑1 (MQ=255) aaaatTTTATGGTAATCCCCCCATCCACCATCTTCTGATCCCCGACGCGGCGGGTTTTGTCCCTG‑CCGCACGTTTg < 2:75678/75‑1 (MQ=255) aaaaTTTATGTTAATCGCACCAATCTCCATCTTCTGATGCCCGACGCGAAGGGTTTGGTGCCTG‑CCGCACGTTTGa < 2:7541227/76‑1 (MQ=255) aaaaTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTG‑CCGCCCGTTTGa > 1:878321/1‑76 (MQ=255) aaaaTTTATGGTAAGCGCCCCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGa < 2:3474329/76‑1 (MQ=255) aaaTTTATGTTAATCGCCCCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAg < 1:3456970/76‑1 (MQ=255) aaaTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTTTGGCTG‑CCGCACGTTTgtg > 1:4386359/1‑74 (MQ=255) tttATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGaa < 2:1123273/76‑1 (MQ=255) ttATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCCCGGTTTAGGAAt > 2:5339665/1‑76 (MQ=255) ttATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTG‑CCGCACGTTTTAGGAAt > 2:7429657/1‑76 (MQ=255) tATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTTTGCCTG‑CCCCCCGTTTTAGGAAtt > 2:1470454/1‑76 (MQ=255) tATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTG‑CCGCACGTTTGAGGAAtt > 2:6370891/1‑76 (MQ=255) aTGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAAttt < 2:3498305/76‑1 (MQ=255) aTGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTG‑CCGCACGGTTTAGGAAttt > 2:2526343/1‑76 (MQ=255) tGGTAACCGCACCTTCCCCCATCTTCTGATCCCCGACGCGGCAGGTTTTGTCCCTG‑CCGCACGTTTGAGGAATTTg < 2:4080838/76‑1 (MQ=255) ggTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGc < 2:7072880/76‑1 (MQ=255) ggTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGCGTCTG‑GCGCCCGGTTGACGAATTTGc > 1:6415452/1‑76 (MQ=255) tAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCCCACGTTTGAGGAATTTGTTa > 1:3632154/1‑76 (MQ=255) tAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTTTGCCTG‑CCGCCCGTTTGAGGGATTTGCTa > 1:7235102/1‑76 (MQ=255) tAACCGCCCCATTCCCCATCTTCTGATGCCCGACGGGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTa < 1:1522836/76‑1 (MQ=255) aaTCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGGATTTGCTAt > 2:6838707/1‑76 (MQ=255) aaTCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTAt > 1:3240131/1‑76 (MQ=255) aaTCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTG‑CCGCACGCTTGAGGAATTTTCTAt > 2:5513985/1‑76 (MQ=255) aaTCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGAGCCTG‑CCGCACGTTTTAGCAATTTGCtct > 2:1766199/1‑74 (MQ=255) aaTCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGATTTTTTTCTT‑GCGGACCTTTGAGGAAATTGCTAt > 2:7676297/1‑76 (MQ=255) cGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTTCCCG‑CCCCGCGTTTGACGAATTTGCTATGaa > 1:6209393/1‑76 (MQ=255) cGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTTTGaa > 1:2826395/1‑76 (MQ=255) cGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCCCACGGTTGAGGAATTTGCTATGaa > 2:5522205/1‑76 (MQ=255) cACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGaaaa > 1:7116106/1‑76 (MQ=255) agcATTCACCATATTCTGATGCCCGACGCGGCAGGGTTTTTGCCTG‑TCGCACGGTTGAGGCATTTCCTCTGaaaaa > 2:3916868/3‑76 (MQ=255) cccATCTTCTGATGCCCGACGCGGCAGGTTTTGTCCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGAtt < 2:5408581/75‑1 (MQ=255) cATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTTAGGAATTTGCTATGAAAAAACTGATtct > 2:4290148/1‑76 (MQ=255) aTCTTCTGATGCCCGACGCGGCAGGGTTTTTGCCTG‑CCGCACCTTTGAGGAATTTGCCATGAAAAAAATGATtttc > 2:4984581/1‑76 (MQ=255) aTCTTCTGATGCCCGACGCGGCAGGGTTTGTGGCTG‑CCGCACGGTTGAGGAATTTGCTATGAAAAAAATGATtctc > 2:4864649/1‑76 (MQ=255) aTCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTG‑CCGCACGGTTGAGGAATTTGCTATGAAAAAACTGATtctc > 2:5087201/1‑76 (MQ=255) tcttctGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAAATTGCTATGAACAAACTGATTCTCa > 2:3167582/1‑76 (MQ=255) ttctGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATc > 2:7225710/1‑76 (MQ=255) ttctGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATc < 2:7465927/76‑1 (MQ=255) tctGATGCCCGACGCGGCAGGGTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCg > 1:4919865/1‑76 (MQ=255) tGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGcc > 2:470803/1‑76 (MQ=255) tGATCCCCGCCGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGcc < 1:3013578/76‑1 (MQ=255) gATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCa > 2:1800223/1‑76 (MQ=255) atccccGCCGGGGCGGTTTTTGTCCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCat < 1:5362674/73‑1 (MQ=255) aTGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCat > 1:1038368/1‑76 (MQ=255) aTGCCCGACGCGGCAGGGTTTTTGCCTG‑CCGCACGTTTTGGGAAATTGCTATTAAAAAACTGATTTTCATGGCCat > 2:7250307/1‑76 (MQ=255) tGCCCGCCGCGGCAGTTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCatg < 2:681954/76‑1 (MQ=255) gCCGGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCatga < 2:3001263/76‑1 (MQ=255) gCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCatga > 1:6911400/1‑76 (MQ=255) gCCCGACGCGGCAGGGTTTGTGCCTG‑CCGCACGTTTTAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCatga > 2:6428173/1‑76 (MQ=255) ccGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCatgatg > 1:7641538/1‑76 (MQ=255) cGACGCGGCAGGGTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATgg > 2:6344212/1‑76 (MQ=255) gcggcgGCAGTTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGc < 2:6467078/73‑1 (MQ=255) gACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGc < 1:1137543/76‑1 (MQ=255) gACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGc > 2:3989399/1‑76 (MQ=255) aCGCGGCGGTTTTGGTCCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCa < 2:4069785/76‑1 (MQ=255) aCGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCa > 2:2643020/1‑76 (MQ=255) gcgGCAGGTTTTGTCCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATc < 2:1648504/76‑1 (MQ=255) gcgGCAGGGTTTTTGGCTG‑CCCCACGTTTTTGGGAATTTCTATGAAAAAACTGATTTTCATCGCCATGATGGCATc > 2:5314760/1‑76 (MQ=255) cgGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCg > 2:1026364/1‑76 (MQ=255) cgGCAGGGTTTGTGCCTG‑GCGCGCGTTTTGAGAAATTGCTATGAAAAAACAGATTCTCATCGCCATGATGGCGTCg > 2:1161989/1‑76 (MQ=255) ggCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATAgg < 2:5738699/76‑1 (MQ=255) cAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCgggg < 2:1236664/76‑1 (MQ=255) aGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGc > 2:3835613/1‑76 (MQ=255) aGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGc > 2:7379112/1‑76 (MQ=255) aGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGc > 1:6591793/1‑76 (MQ=255) gTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCTg > 2:5628216/1‑76 (MQ=255) gTTTTGTGCCCGCCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCt < 1:6017762/76‑1 (MQ=255) ttttGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCTgg > 1:1940753/1‑76 (MQ=255) ttttGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCTgg > 2:2309054/1‑76 (MQ=255) ttttGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCTgg > 2:7685949/1‑76 (MQ=255) | TCTTCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTG‑CCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCTGG > REL606/691777‑691927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |