| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,395 | T→C | S354P (TCC→CCC) | thrA → | bifunctional aspartokinase I/homeserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,395 | 0 | T | C | 99.5% | 927.2 / ‑4.4 | 219 | S354P (TCC→CCC) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (109/109); total (109/110) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
Only 100 of 230 total aligned reads displayed.
GGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGT > REL606/1323‑1467 | ggCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCg < 1:5463975/76‑1 (MQ=255)ggCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCg < 1:2546027/76‑1 (MQ=255) cATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGaa < 2:3195836/76‑1 (MQ=255) aTGGCGGCGCGCGTGTTTGCAGCGATGTCACGCTCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAAt < 2:332870/76‑1 (MQ=255) ggcggcGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATAc > 1:3699849/1‑76 (MQ=255) gcgcgcGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTa > 2:6740969/1‑76 (MQ=255) cgcgcgTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTAt > 2:3934676/1‑76 (MQ=255) gcgTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAg < 2:6484757/76‑1 (MQ=255) cgTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGt < 2:1714359/76‑1 (MQ=255) cgTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGt < 1:3412603/76‑1 (MQ=255) gtgtTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGtt > 2:189301/1‑76 (MQ=255) tttGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGGTTACGCAATCATCTCCCGAATACAGTATCAGTTTCt > 2:5971540/1‑76 (MQ=255) tttGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCt < 2:5243581/76‑1 (MQ=255) cGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTcc > 1:65835/1‑76 (MQ=255) gATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCg > 2:7622628/1‑76 (MQ=255) gATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCg > 2:1603673/1‑76 (MQ=255) tGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCa > 2:2062678/1‑76 (MQ=255) tGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCa > 1:5636189/1‑76 (MQ=255) tGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCa > 2:2892084/1‑76 (MQ=255) tCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGCATACAGTATCAGTTTCTGCGTTCCGCaac > 1:3455773/1‑75 (MQ=255) tCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCaaa < 2:4185407/76‑1 (MQ=255) tCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCaaa < 2:4432657/76‑1 (MQ=255) tCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCaaa < 2:5065696/76‑1 (MQ=255) cACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAg > 2:722591/1‑76 (MQ=255) aCGCTCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGc < 1:6505705/76‑1 (MQ=255) aCGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGc < 1:7509298/76‑1 (MQ=255) gcCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACt > 2:152916/1‑76 (MQ=255) gcCCGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAACCGACt > 2:1633021/1‑76 (MQ=255) cccGTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACtg < 1:1248771/76‑1 (MQ=255) gTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACtgtgt > 2:6596661/1‑76 (MQ=255) gTATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACtgtgt > 1:6043701/1‑76 (MQ=255) tATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACtgtgtg > 2:530212/1‑76 (MQ=255) tATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACtgtgtg > 1:3504044/1‑76 (MQ=255) tATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACtgtgtg > 2:4789081/1‑76 (MQ=255) tATTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACtgtgtg > 2:3819533/1‑76 (MQ=255) aTTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGc > 1:4528922/1‑76 (MQ=255) aTTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGc > 1:7708857/1‑76 (MQ=255) aTTTCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGc < 1:4159380/76‑1 (MQ=255) tttCCGTGGTGCTGATTACGCAATCGTCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCg < 1:7820043/76‑1 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCg < 2:397807/76‑1 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCg < 1:6794013/76‑1 (MQ=255) tCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAg > 2:6655974/1‑76 (MQ=255) tCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAg < 2:5398628/76‑1 (MQ=255) tCCGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAg > 1:4761526/1‑76 (MQ=255) ccGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGc > 2:5724022/1‑76 (MQ=255) ccGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGc > 1:783592/1‑76 (MQ=255) ccGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGc > 1:1971531/1‑76 (MQ=255) cGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCt < 2:576754/76‑1 (MQ=255) cGTGGTGCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCt > 1:7264291/1‑76 (MQ=255) gtggtgCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTg < 1:1049502/76‑1 (MQ=255) gtggtgCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTg > 2:4697083/1‑76 (MQ=255) gtggtgCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTg < 1:1466464/76‑1 (MQ=255) tggtgCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGa < 1:5726175/76‑1 (MQ=255) ggtgCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGaa < 1:7121668/76‑1 (MQ=255) ggtgCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGaa < 1:3335741/76‑1 (MQ=255) gtgCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc > 1:7684854/1‑76 (MQ=255) gtgCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc < 1:6963333/76‑1 (MQ=255) gtgCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc < 1:2968610/76‑1 (MQ=255) tgCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACg > 1:4404013/1‑76 (MQ=255) gCTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACgg > 1:6601583/1‑76 (MQ=255) cTGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACggg < 1:1700521/76‑1 (MQ=255) tGATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGc > 1:5299679/1‑76 (MQ=255) gATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCa > 1:4887743/1‑76 (MQ=255) gATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCa < 1:4320790/76‑1 (MQ=255) gATTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCa > 1:1987873/1‑76 (MQ=255) gATTACGCAATCATCTCCAGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCa > 2:7672274/1‑76 (MQ=255) aTTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCaa > 1:220740/1‑76 (MQ=255) aTTACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGCGTGCGAGCTGAACGGGCaa > 2:6461183/1‑76 (MQ=255) ttACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAAt > 1:2637554/1‑76 (MQ=255) tACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATg > 1:2815201/1‑76 (MQ=255) tACGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATg > 1:242945/1‑76 (MQ=255) aCGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc < 2:352155/76‑1 (MQ=255) aCGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc < 2:273784/76‑1 (MQ=255) aCGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc < 1:6740969/76‑1 (MQ=255) aCGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc > 2:6922464/1‑76 (MQ=255) aCGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc < 1:5360647/76‑1 (MQ=255) aCGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc < 1:2084106/76‑1 (MQ=255) cGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCa > 2:6112296/1‑76 (MQ=255) cGCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCa > 1:1330024/1‑76 (MQ=255) gCAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGCCTGTGTGCGAGCTGAACGGGCAATGCAg < 2:6039863/76‑1 (MQ=255) cAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg > 2:5187902/1‑76 (MQ=255) cAATCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg < 1:3107993/76‑1 (MQ=255) aaTCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGa < 2:4065218/76‑1 (MQ=255) aaTCATCTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGAAATGCAGGa < 2:71508/76‑1 (MQ=255) atcatcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGaa < 1:6262110/76‑1 (MQ=255) atcatcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGaa > 1:1180090/1‑76 (MQ=255) atcatcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGaa < 2:3070734/76‑1 (MQ=255) tcatcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAag < 2:2951933/76‑1 (MQ=255) tcatcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAag < 1:352014/76‑1 (MQ=255) tcatcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAag < 1:4092383/76‑1 (MQ=255) catcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAaga > 1:3274063/1‑76 (MQ=255) catcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAaga < 1:5876711/76‑1 (MQ=255) catcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAaga > 2:4541623/1‑76 (MQ=255) catcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAaga > 1:1589333/1‑76 (MQ=255) catcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAaga > 2:1212234/1‑76 (MQ=255) catcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAaga > 2:1424154/1‑76 (MQ=255) atcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAagag < 1:4941993/76‑1 (MQ=255) atcTCCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAagag > 2:499088/1‑76 (MQ=255) tctcCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGt < 1:2790244/76‑1 (MQ=255) tctcCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGt > 2:5691167/1‑76 (MQ=255) | GGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGT > REL606/1323‑1467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |