| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,161,948 | G→A | E365K (GAA→AAA) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,161,948 | 0 | G | A | 99.6% | 922.0 / ‑3.2 | 223 | coding (1093/4029 nt) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (158/64); minor base . (0/1); total (158/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.21e-01 | |||||||||||
Only 100 of 263 total aligned reads displayed.
TCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGT > REL606/4161875‑4162020 | tCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTaaaa < 2:4688028/76‑1 (MQ=255)tCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTaaaa < 2:2935881/76‑1 (MQ=255)tCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTaaaa > 2:7262305/1‑76 (MQ=255) ggATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATc > 2:69921/1‑76 (MQ=255) ggATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATc > 2:2076392/1‑76 (MQ=255) ggATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATc > 2:4492112/1‑76 (MQ=255) gATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCt < 2:4909260/76‑1 (MQ=255) aTCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTa > 2:2131764/1‑76 (MQ=255) cACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTAcc > 1:1078692/1‑76 (MQ=255) cACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTAcc > 2:2820061/1‑76 (MQ=255) aCGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCg < 1:2521745/76‑1 (MQ=255) ggCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCa > 1:4289042/1‑76 (MQ=255) gCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCat > 2:1488432/1‑76 (MQ=255) gCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCat > 2:4254389/1‑76 (MQ=255) cccATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatg > 1:5240012/1‑76 (MQ=255) ccATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatga > 1:4057065/1‑76 (MQ=255) cATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatgat > 1:2527485/1‑76 (MQ=255) atatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatgatg > 2:7095371/1‑76 (MQ=255) atatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatgatg < 1:2866679/76‑1 (MQ=255) atatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatgatg > 2:1070554/1‑76 (MQ=255) tatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATgc < 1:3710416/76‑1 (MQ=255) tatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATgc > 1:2435700/1‑76 (MQ=255) tatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATgcgc > 1:786533/1‑76 (MQ=255) atCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGcc > 1:1548067/1‑76 (MQ=255) tctcTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGccc > 2:1895393/1‑76 (MQ=255) tctcTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGccc > 2:1404932/1‑76 (MQ=255) tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTg > 1:1741675/1‑76 (MQ=255) tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTg > 1:129607/1‑76 (MQ=255) cctGAAACCCTACGGGGCGACCCAACCAACCGCCGTCTGAGCGCACCGGTAAAAATCTACCGCATGATGCGCCCTg < 1:701111/75‑1 (MQ=255) ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTgg > 2:3703242/1‑76 (MQ=255) ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTgg > 2:5192490/1‑76 (MQ=255) ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTgg > 1:3090839/1‑76 (MQ=255) ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTgg > 2:3313206/1‑76 (MQ=255) ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTgg > 2:2977746/1‑76 (MQ=255) tGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGc < 1:4126420/76‑1 (MQ=255) tGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGc < 2:2383991/76‑1 (MQ=255) aaaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGa > 2:6804424/1‑76 (MQ=255) aaaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGa < 1:1469018/76‑1 (MQ=255) aaaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGa < 1:1778584/76‑1 (MQ=255) aaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAg > 1:1518915/1‑76 (MQ=255) aCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgc > 2:6025647/1‑76 (MQ=255) ccTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgcc > 2:2210049/1‑76 (MQ=255) ccTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgcc > 1:4911538/1‑76 (MQ=255) cTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgccg > 2:6591749/1‑76 (MQ=255) tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgccgcc > 1:6428302/1‑76 (MQ=255) tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgccgcc > 2:7639436/1‑76 (MQ=255) tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgccgcc > 2:7551758/1‑76 (MQ=255) ccGTGTCGCCCCAACTACCGCCCGTCGGAGCGCACTGGAAAAAATCTCCCGCATGATGCCCCCGGGCGAGccgccg < 2:6301460/75‑1 (MQ=255) gtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAACAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTc > 2:3109896/1‑76 (MQ=255) gtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTc < 1:6317875/76‑1 (MQ=255) tCGACCAAACTAACGACCGTCGGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCg < 2:573819/76‑1 (MQ=255) gACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTg < 1:7569032/76‑1 (MQ=255) aCCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGa < 1:1209359/76‑1 (MQ=255) cccAACAAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGaa < 1:357929/76‑1 (MQ=255) cAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTCCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagc < 2:7041517/76‑1 (MQ=255) cAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagc < 1:3872709/76‑1 (MQ=255) cAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagc < 1:2022339/76‑1 (MQ=255) aaCTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagca < 2:666165/76‑1 (MQ=255) aaCTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagca > 2:5421169/1‑76 (MQ=255) aCTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagcag > 1:1171105/1‑76 (MQ=255) aCTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagcag > 1:186694/1‑76 (MQ=255) aCTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagcag > 1:5889529/1‑76 (MQ=255) cTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagcagc > 1:4861344/1‑76 (MQ=255) cTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagcagc > 2:6161944/1‑76 (MQ=255) cTAACGACCGTCTGAGAGCACTGGTAAAAATGTACCGCATGATGCGCCCTGGGGAGCCGCCGGCTCGTGAagcagc < 2:7804977/76‑1 (MQ=255) tAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCg > 2:1347729/1‑76 (MQ=255) aaCGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 2:363210/1‑76 (MQ=255) aaCGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 2:4279566/1‑76 (MQ=255) aaCGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 2:6266978/1‑76 (MQ=255) aaCGACCGTCTGAGCACACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 1:6762871/1‑76 (MQ=255) aCGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGa < 1:5723318/76‑1 (MQ=255) cGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaa < 2:1763287/76‑1 (MQ=255) cGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaa > 1:322064/1‑76 (MQ=255) gACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa > 1:55021/1‑76 (MQ=255) gACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa > 2:494460/1‑76 (MQ=255) gACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa > 1:5825602/1‑76 (MQ=255) gACCGTCTGAGCGCACTGGAAAAAATCTCCCGCATGAGGCCCCCGGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa < 2:1669902/76‑1 (MQ=255) aCCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAg < 1:1579796/76‑1 (MQ=255) aCCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAg > 2:3933983/1‑76 (MQ=255) aCCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAg < 1:7084340/76‑1 (MQ=255) gccTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt < 2:1115460/74‑1 (MQ=255) gTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt < 1:6650871/76‑1 (MQ=255) gTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt > 2:5119283/1‑76 (MQ=255) gTCTGAGCGCACTGGTAAAAATCTACCGCATGAGGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt < 2:5924325/76‑1 (MQ=255) tCTGAGCGCACTGGTAAAAATCTCCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTg < 1:7262305/76‑1 (MQ=255) cTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGt > 1:979704/1‑76 (MQ=255) tGAGCGCACTGGGAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt > 2:7505294/1‑76 (MQ=255) tGAGCCCACTGGTAAAAATCTCCCGCATGATGCCCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt < 1:7346440/76‑1 (MQ=255) gAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTc > 1:3785487/1‑76 (MQ=255) gAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTc > 2:437251/1‑76 (MQ=255) gcgcACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCga < 2:7243792/76‑1 (MQ=255) gcgcACTGGTAAAAATCTACCGCATGATGCCCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCga < 2:5542445/76‑1 (MQ=255) cgcACTGGTAAAAATCTACCGCATGATGCCCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgag < 1:883196/76‑1 (MQ=255) gcACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgaga < 1:6914084/76‑1 (MQ=255) cACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGaa < 1:6111401/76‑1 (MQ=255) cTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAAcc > 2:2604735/1‑76 (MQ=255) cTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAAcc > 1:347469/1‑76 (MQ=255) gggTAAAAATCTCCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCt < 1:445446/75‑1 (MQ=255) ggTAAAAATCTCCCGCATGATGCGCCCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTg < 2:6005155/76‑1 (MQ=255) ggTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTg < 2:2525786/76‑1 (MQ=255) | TCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGT > REL606/4161875‑4162020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |