| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 475,187 | C→T | G40D (GGT→GAT) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 475,187 | 0 | C | T | 98.8% | 639.0 / ‑2.3 | 166 | G40D (GGT→GAT) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (93/71); total (93/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
Only 100 of 184 total aligned reads displayed.
GCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCA > REL606/475114‑475259 | gCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACAtcc > 1:933210/1‑76 (MQ=255) cccAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCg > 1:7034472/1‑76 (MQ=255) ccAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGc > 2:7788813/1‑76 (MQ=255) cAGTTCCCCCAGTCCCGGGGAAAGCTTGGTATCGGCAACAAACCCCGGAGTGAATGCTCCTGCCAGCACATCCGCt < 2:334820/76‑1 (MQ=255) cAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCt > 2:892161/1‑76 (MQ=255) cAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCt > 2:3087503/1‑76 (MQ=255) aGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGcca > 2:5271256/1‑74 (MQ=255) aGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTa > 2:1568979/1‑76 (MQ=255) gtccAGCCATTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTaa < 1:4017045/73‑1 (MQ=255) gtccAGCCAGTTCCGGGGCAAGCTGGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTaa < 1:5965113/73‑1 (MQ=255) ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAAc > 1:1083924/1‑76 (MQ=255) ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAAc > 2:5744882/1‑76 (MQ=255) ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGGCCTGCCAGCACATCCGCTAAc > 1:2166928/1‑76 (MQ=255) ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGAGAATGGTCCTGCCAGCACATCCGCTAAc > 2:1615744/1‑76 (MQ=255) tccAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGCCCTGCCAGCACATCCGCTAAc < 1:3212186/74‑1 (MQ=255) cAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAg > 1:266707/1‑76 (MQ=255) cAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAg > 2:6232681/1‑76 (MQ=255) cAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAg > 1:4462349/1‑76 (MQ=255) cAGCCAGTCCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAg < 1:5149843/76‑1 (MQ=255) gCCAGTCCGGGGGAAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGCCCTGCCAGCACATCCGCTAACAGat < 1:7124882/76‑1 (MQ=255) ccAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCCGCACATCCGCTAACAGata > 2:1167564/1‑76 (MQ=255) ccAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCACGCACATCCGCTAACAGata > 2:1083333/1‑76 (MQ=255) ccAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGGCCTGCCAGCACATCCGCTAACAGata > 2:4080080/1‑76 (MQ=255) cAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGatat > 2:1470607/1‑76 (MQ=255) cAGTCCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGatat < 1:2644750/76‑1 (MQ=255) aGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGGCAGCACATCCGCTAACAGATATc > 1:6689974/1‑76 (MQ=255) aGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATc > 1:3605961/1‑76 (MQ=255) gTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATcc > 2:6788041/1‑76 (MQ=255) gTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATcc > 2:5560705/1‑76 (MQ=255) tCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAAATATCcca < 1:5196708/76‑1 (MQ=255) cgggggCAAGCTTGGTATCGGCAACAAGGCCCGGAGTAAATGGCCCTGCCAGCACATCCGCTAACAGATATCccac < 2:496957/74‑1 (MQ=255) cGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCCGAACATCCGCTAACAGATTTCccacc > 2:127616/1‑76 (MQ=255) cGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCccacc < 1:497200/76‑1 (MQ=255) cGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCccacc < 1:5340926/76‑1 (MQ=255) cGGGGAAAGCTTGTTATCGGCAACAAAGCCCGGAGTGAATGGCCCTGCCAGCACATCCGCTAACAGATATCccacc < 1:6275617/76‑1 (MQ=255) ggaaaGCTGGGTACCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCaga < 1:4335804/73‑1 (MQ=255) gCAAGTTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCagag < 1:4566990/76‑1 (MQ=255) cAAGCTGGGTAGCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAgagg < 1:3485922/76‑1 (MQ=255) aaGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAgagga > 2:521104/1‑76 (MQ=255) ttGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGaaa > 1:1374202/1‑76 (MQ=255) tGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAAt > 1:3566996/1‑76 (MQ=255) tGGTATCGGCAACAAAGCCCGGAGTGAATGGCCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAAt < 1:4530846/76‑1 (MQ=255) gggTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAAt < 1:6368733/75‑1 (MQ=255) gggTATCGGAAACAAAGCCCGGAGTGAAGGGCCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAAt < 2:2511685/75‑1 (MQ=255) gttATGGGAAACAAAGCCGGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATa < 1:2480904/74‑1 (MQ=255) ggTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATa > 1:4692658/1‑76 (MQ=255) gTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATAc > 2:6090548/1‑76 (MQ=255) tATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACg > 1:5551960/1‑76 (MQ=255) tATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACg > 2:6298841/1‑76 (MQ=255) tATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACg > 2:3608700/1‑76 (MQ=255) tATCGGCAACAAAGCCCGGAGTGAAGGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACg < 2:7438105/76‑1 (MQ=255) aTCGGAAACAAAGCCCGGAGTAAATGGCCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGt < 2:2032/76‑1 (MQ=255) ggCAACAAAGCCCGGAGTGAATGGTCCTGCCAACACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTatt > 2:4320247/1‑74 (MQ=255) ggCAACAAAGCCCGGAGTGAATGGCCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGt < 1:5771615/76‑1 (MQ=255) caaaaaACCCGGGAGTGAATGGCCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGttt < 2:3446205/76‑1 (MQ=255) aacaaAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTa > 2:160946/1‑76 (MQ=255) acaaACCCGGGAGGAAAGGGCCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAt < 1:3444009/76‑1 (MQ=255) caaAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAtt > 2:3815240/1‑76 (MQ=255) caaAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAtt > 2:4393953/1‑76 (MQ=255) caaAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAtt > 2:6324061/1‑76 (MQ=255) caaAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAtt > 1:5951882/1‑76 (MQ=255) caaAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAtt > 1:3985446/1‑76 (MQ=255) aaaGCCCGGAGTGAATGGTCCTGCCAGTACATCCGCTAACAGATATACCACCAGAGGAGAAATACGCAGTTTATTg > 2:4480457/1‑76 (MQ=255) aaaGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTg > 2:2076619/1‑76 (MQ=255) aaGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTgg > 2:1949439/1‑76 (MQ=255) aaGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTgg > 2:7176544/1‑76 (MQ=255) aGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGTAGAAATACGTAGTTTATTGGc > 1:6044495/1‑76 (MQ=255) aGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGTAGAAATACGTAGTTTATTGGc > 1:6286935/1‑76 (MQ=255) gCCGGGAGTAAATGGCCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGcc < 1:7521764/76‑1 (MQ=255) gCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGcc < 2:5699834/76‑1 (MQ=255) gCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGcc > 1:4466835/1‑76 (MQ=255) gCCCGGAGTGAATGGCCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGcc < 1:1063597/76‑1 (MQ=255) cccGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCa > 1:6773826/1‑76 (MQ=255) cccGGAGTGAATGGTCCTGCCACCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCa < 1:1723227/76‑1 (MQ=255) cccGGAGTGAAGGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCa < 1:2315334/76‑1 (MQ=255) ccGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAg > 2:7350262/1‑76 (MQ=255) ccGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAg > 2:5326098/1‑76 (MQ=255) ccGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAg > 1:6415981/1‑76 (MQ=255) cGGAGTGAATGGTCCTGCCAGCAAATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTTGCCatc > 2:2711879/1‑74 (MQ=255) aGTGAAGGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATg < 1:1910029/76‑1 (MQ=255) gTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGc > 2:1463203/1‑76 (MQ=255) tGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGcc > 1:1858176/1‑76 (MQ=255) gAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCg > 1:6437981/1‑76 (MQ=255) gAATGGCCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCg < 1:1056163/76‑1 (MQ=255) ggcccTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGa < 2:1545817/73‑1 (MQ=255) ggTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGa > 1:7218772/1‑76 (MQ=255) gTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGAt > 2:5468507/1‑76 (MQ=255) tCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATa > 2:473429/1‑76 (MQ=255) cccTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATa < 1:1485931/75‑1 (MQ=255) ccTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATaa < 1:5230409/76‑1 (MQ=255) ccTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATaa < 1:3690151/76‑1 (MQ=255) cTGCCAGCTCAACCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATaaa < 1:4599460/76‑1 (MQ=255) cTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATaaa < 2:6098519/76‑1 (MQ=255) gCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAgg > 2:6983095/1‑76 (MQ=255) cAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCg > 2:4426758/1‑76 (MQ=255) cAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCg < 1:6527105/76‑1 (MQ=255) cacaTCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGc < 1:5022435/76‑1 (MQ=255) cacaTCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGc < 1:3891537/76‑1 (MQ=255) cacaTCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGc > 2:6902947/1‑76 (MQ=255) cacaTCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGc < 2:456776/76‑1 (MQ=255) | GCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCA > REL606/475114‑475259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |