BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,516	0	C	T	100.0%	708.9 / NA	176	H33Y (CAC→TAC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (92/84); total (92/84)

Only 100 of 206 total aligned reads displayed.

TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588

tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac                                                                        <  1:1178682/76‑1 (MQ=255)

tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac                                                                        >  1:5451988/1‑76 (MQ=255)

tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac                                                                        <  2:2206168/76‑1 (MQ=255)

tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac                                                                        <  1:2219528/76‑1 (MQ=255)

tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac                                                                        <  2:6903439/76‑1 (MQ=255)

tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCATCGGTtac                                                                        <  1:1646936/76‑1 (MQ=255)

  aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                      >  2:7759106/1‑76 (MQ=255)

     ccccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcg                                                                   >  1:3220961/1‑76 (MQ=255)

     ccccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcg                                                                   >  2:1637235/1‑76 (MQ=255)

      cccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgc                                                                  <  1:134082/76‑1 (MQ=255)

       ccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgcg                                                                 <  1:3260966/76‑1 (MQ=255)

        cGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGa                                                                <  2:6795323/76‑1 (MQ=255)

          gCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAtt                                                              <  2:5189613/76‑1 (MQ=255)

          gCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAtt                                                              <  2:1147447/76‑1 (MQ=255)

            aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                            >  2:6892770/1‑76 (MQ=255)

            aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                            >  2:4843137/1‑76 (MQ=255)

            aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                            <  2:3092737/76‑1 (MQ=255)

             aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                           >  1:4838275/1‑76 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      <  2:5107037/76‑1 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      <  2:7245801/76‑1 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      >  2:810947/1‑76 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      >  1:2164547/1‑76 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      >  1:3156036/1‑76 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATCTGCCGAcc                                                      >  2:1931156/1‑76 (MQ=255)

                   aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                     >  2:2463849/1‑76 (MQ=255)

                   aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                     <  2:6737800/76‑1 (MQ=255)

                    aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                    <  2:5971357/76‑1 (MQ=255)

                    aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                    >  1:4315088/1‑76 (MQ=255)

                    aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                    >  1:5207321/1‑76 (MQ=255)

                    aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTAAATCCGCGATTTACCAAACAg                                                    >  2:4886206/1‑76 (MQ=255)

                    aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAAGCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                    <  2:4379668/76‑1 (MQ=255)

                        gATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGc                                                <  1:1920205/76‑1 (MQ=255)

                         aTCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCt                                               >  2:4720076/1‑76 (MQ=255)

                           caacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCa                                             >  2:6524397/1‑76 (MQ=255)

                            aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg                                            >  2:7774657/1‑76 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  2:3186001/76‑1 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGACGGCCAGAGTCTCATCt                                          >  2:2078919/1‑76 (MQ=255)

                               aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg                                         <  1:244531/76‑1 (MQ=255)

                               aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg                                         >  1:5703261/1‑76 (MQ=255)

                               aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg                                         >  1:3968639/1‑76 (MQ=255)

                                 gTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAgccgcc                                       >  2:4490760/1‑76 (MQ=255)

                                 gTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGcc                                       >  1:2403485/1‑76 (MQ=255)

                                  tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa                                      <  1:2953298/76‑1 (MQ=255)

                                   aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa                                     >  2:1768664/1‑76 (MQ=255)

                                   aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa                                     >  2:6377087/1‑76 (MQ=255)

                                   aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa                                     >  1:6669544/1‑76 (MQ=255)

                                    tCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaa                                    >  1:7787981/1‑76 (MQ=255)

                                     cTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaa                                   <  1:7593882/76‑1 (MQ=255)

                                      tGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaaa                                  >  2:3242820/1‑76 (MQ=255)

                                      tGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAACTGCCaaaaa                                  >  1:586732/1‑76 (MQ=255)

                                       gggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCcaaaaa                                 >  2:2176607/1‑75 (MQ=255)

                                       gggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaaaa                                 >  2:4218907/1‑75 (MQ=255)

                                       gggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAag                                 >  2:3507655/1‑76 (MQ=255)

                                       gggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAag                                 <  2:2143816/76‑1 (MQ=255)

                                        ggTAGTGACTACTTGGTGAAACCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAaga                                <  2:5919920/76‑1 (MQ=255)

                                          tAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGt                              <  2:5003723/76‑1 (MQ=255)

                                           aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                             <  1:3319566/76‑1 (MQ=255)

                                           aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                             <  1:1705727/76‑1 (MQ=255)

                                           aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                             >  1:5267809/1‑76 (MQ=255)

                                            gTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGc                            >  1:2079792/1‑76 (MQ=255)

                                            gTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGc                            <  1:4485469/76‑1 (MQ=255)

                                              gACTACGTGGTGAAATCCAGCGTCGTTTACATCCGTGATTTGCGGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg                          <  2:3583052/76‑1 (MQ=255)

                                              gACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg                          <  1:3547589/76‑1 (MQ=255)

                                              gACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg                          >  1:5532388/1‑76 (MQ=255)

                                              gACTACGTGGGGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg                          <  2:3841577/76‑1 (MQ=255)

                                                cTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAc                        >  1:7175603/1‑76 (MQ=255)

                                                 tACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAct                       >  1:7324397/1‑76 (MQ=255)

                                                  aCGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActc                      >  2:2561478/1‑76 (MQ=255)

                                                  aCGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActc                      >  1:7391877/1‑76 (MQ=255)

                                                   ctgggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     <  2:2962773/73‑1 (MQ=255)

                                                   cGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     <  1:4523342/76‑1 (MQ=255)

                                                   cGTGGGAAAACCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     <  2:4798288/76‑1 (MQ=255)

                                                    gtggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    <  1:4600791/76‑1 (MQ=255)

                                                    gtggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    <  2:7676802/76‑1 (MQ=255)

                                                      ggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGGCTCTAcc                  >  2:4734467/1‑76 (MQ=255)

                                                      ggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTAcc                  >  1:1084869/1‑76 (MQ=255)

                                                      ggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTAcc                  >  1:574921/1‑76 (MQ=255)

                                                        tgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGCCCCGTGGCTCAGCTGCCAAAAAGAAGGCCGGctcctcctc                >  2:361554/1‑76 (MQ=255)

                                                        tgAAACCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTc                <  2:6267074/76‑1 (MQ=255)

                                                          aaaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcca              >  1:3840905/1‑76 (MQ=255)

                                                          aaaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcca              <  1:6438098/76‑1 (MQ=255)

                                                           aaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccac             <  1:1596164/76‑1 (MQ=255)

                                                            acccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc            <  2:5063907/74‑1 (MQ=255)

                                                            acccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc            <  1:4195384/74‑1 (MQ=255)

                                                             tCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacca           <  1:265393/76‑1 (MQ=255)

                                                             cccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacca           <  2:105831/75‑1 (MQ=255)

                                                               cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  1:3649045/76‑1 (MQ=255)

                                                               cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  1:5814098/76‑1 (MQ=255)

                                                               cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  1:1964229/76‑1 (MQ=255)

                                                               cAGCGGCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  2:7567563/76‑1 (MQ=255)

                                                                 gCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGAc       <  1:1768664/76‑1 (MQ=255)

                                                                 gCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGAc       >  2:5459805/1‑76 (MQ=255)

                                                                  ggTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACg      <  1:4777284/75‑1 (MQ=255)

                                                                  cGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACg      <  1:6365907/76‑1 (MQ=255)

                                                                  cGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACg      <  1:1614453/76‑1 (MQ=255)

                                                                   gTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACgg     <  1:922658/76‑1 (MQ=255)

                                                                    tCGGTTACATCCGCGTTTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGc    <  2:707163/76‑1 (MQ=255)

                                                                     cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   <  1:5992307/76‑1 (MQ=255)

                                                                     cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   <  1:4063937/76‑1 (MQ=255)

                                                                      ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  1:850203/76‑1 (MQ=255)

TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`