| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,779 | 0 | G | T | 99.4% | 595.2 / NA | 155 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (72/81); total (72/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 181 total aligned reads displayed.
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 | gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGGAAATAGCCCCCAAAACAACAAAACCCCCATccccc > 1:595025/1‑75 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGAAATCAGCCCCCAAAACAGCAAAACGCCAATCCccac > 1:5788800/1‑76 (MQ=255) tGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGGAATCAGCCCCAATAACAGCAAAAAGCCCAACCccacc > 1:3668085/1‑76 (MQ=255) tGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGGAAACAGCCCCAAAAACAGCAAAACGCCAAAACccacc > 1:2391914/1‑76 (MQ=255) gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGGAAAACCG‑CCCAATAAAAACAAAAAGCCAAAAAccacca > 2:7697201/1‑76 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg < 1:3240716/76‑1 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGGAAACAGCCCCCAAAAAAACAAAAAGCCCAACCCCACCAGa > 1:7461366/1‑76 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa < 1:4389280/76‑1 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGTAAATAGACCCCATAAAAGCAAAACACCAAACCccacccac > 1:1846327/1‑73 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa < 2:1635151/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGGC‑GGGAAACACCCCCCATAACAGCAAAAACCCAAATCCCCCCAGATa > 2:4771022/1‑76 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 1:5153346/76‑1 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 1:6627997/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGGCTGGGTGTC‑GGGAAACAGGCCCCAAAACCGCCAAAAGCCAAACCCCCCCCCATaaaaa > 2:6848287/1‑74 (MQ=255) gTCCAGGCGCGGCTGGTCTGGCGGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa < 1:453178/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 2:3259639/76‑1 (MQ=255) ccAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg < 2:127981/76‑1 (MQ=255) cAGGCGCGGCTGGGCTGGGTGGC‑GGGAAGCAGACCCCCAAACAGCAAAACACCCATACCCCCCAGATAAAGCACGa > 2:1965300/1‑76 (MQ=255) cAGGCGCGGCTGGGCTGGCCGTC‑GGGAAACAGCCCCCCTAAAAGCAAAAAGCCAATCCCCCCCACACAAAGAAtaa > 2:5252530/1‑74 (MQ=255) aGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc < 1:7182818/76‑1 (MQ=255) aGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATAAc < 2:3644999/76‑1 (MQ=255) cgcgGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGcc < 1:2923006/76‑1 (MQ=255) cgGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:643401/76‑1 (MQ=255) ggCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt > 1:40460/1‑76 (MQ=255) ggCTGGTCTGGCTGTC‑GGAAATCAGCCCCAAGAACAGCAAAACGCCACTCCCCACCAGATAAAGCATGACGCCTGt < 2:558826/76‑1 (MQ=255) gCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa > 2:4359408/1‑76 (MQ=255) tGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 2:1075072/76‑1 (MQ=255) tGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:2241537/76‑1 (MQ=255) tGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt > 1:2017382/1‑76 (MQ=255) tGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 2:2825769/76‑1 (MQ=255) ggTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 1:3288782/76‑1 (MQ=255) ggTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 2:1224786/1‑76 (MQ=255) ggTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 1:1986476/1‑76 (MQ=255) gTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:4452195/76‑1 (MQ=255) tCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 1:5796158/76‑1 (MQ=255) tCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 2:412108/76‑1 (MQ=255) cTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc < 2:5465263/76‑1 (MQ=255) cTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 2:2061615/1‑76 (MQ=255) cTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 2:2525856/1‑76 (MQ=255) cTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc < 1:1523570/76‑1 (MQ=255) tGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa < 1:6534165/76‑1 (MQ=255) ggCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 1:3473571/76‑1 (MQ=255) gCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg > 1:7408450/1‑76 (MQ=255) gCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg < 1:6868811/76‑1 (MQ=255) gCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg < 2:256547/76‑1 (MQ=255) gCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg < 2:2197126/76‑1 (MQ=255) gCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg > 1:3567906/1‑76 (MQ=255) gTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt > 2:812823/1‑76 (MQ=255) gTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt > 2:3796033/1‑76 (MQ=255) tC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 2:2330781/76‑1 (MQ=255) ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:4018988/76‑1 (MQ=255) gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa > 2:2869691/1‑76 (MQ=255) gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:7452835/76‑1 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc < 1:7160034/76‑1 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc < 2:1809842/76‑1 (MQ=255) aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg > 2:6569105/1‑76 (MQ=255) aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 2:1476670/76‑1 (MQ=255) aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 2:5378622/76‑1 (MQ=255) tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa < 2:6374430/76‑1 (MQ=255) tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa > 2:6355435/1‑76 (MQ=255) tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa > 2:926417/1‑76 (MQ=255) tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa > 2:2496233/1‑76 (MQ=255) aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg < 2:5707117/76‑1 (MQ=255) aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg < 1:7758412/76‑1 (MQ=255) aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg > 2:4107132/1‑76 (MQ=255) gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg > 1:7497505/1‑76 (MQ=255) gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg < 2:2758602/76‑1 (MQ=255) gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg < 2:4981006/76‑1 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc > 1:4192882/1‑76 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc > 1:3341690/1‑76 (MQ=255) cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg > 1:4888613/1‑76 (MQ=255) cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg > 2:573814/1‑76 (MQ=255) cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg > 2:4954833/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 2:6453472/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 2:4570051/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 2:1520373/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 2:7585561/1‑76 (MQ=255) aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGAAt > 1:6263121/1‑76 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc < 1:3688956/76‑1 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:4577500/1‑76 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 1:7463911/1‑76 (MQ=255) aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg < 1:6964212/76‑1 (MQ=255) gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc < 1:5362172/76‑1 (MQ=255) gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc < 1:1480980/76‑1 (MQ=255) gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc < 2:6180259/76‑1 (MQ=255) gCAAAACGCCAATCCCCACCAGATAAAGCATGACACCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc < 2:4566059/76‑1 (MQ=255) cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc > 2:5087362/1‑76 (MQ=255) cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc < 2:4346339/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:1144093/76‑1 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 1:3775578/76‑1 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg > 1:6683072/1‑76 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc < 1:5047900/76‑1 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc > 2:6974950/1‑76 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc > 1:3119009/1‑76 (MQ=255) cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg > 2:740211/1‑76 (MQ=255) gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc > 1:2619978/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAtccgcc > 2:3357283/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 2:1661946/1‑76 (MQ=255) ccAATCCCCACCAGAAAAAGCATGACGCCTGAATTAAACAGGCTTTTTACCGTTGGCCCACCGCGCCCCAgccgcc < 2:3885538/76‑1 (MQ=255) cAACCCCCACAAGATAAAGCATGACCCCTGTATTAAACAGGTTTTTTCCCGAGGGCGCACCGCGCCCCAGCCGCca < 2:4727852/76‑1 (MQ=255) | GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |