Predicted mutation
evidence seq id position mutation annotation gene description
MC JC REL606 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3893602–3894994 3901930 6937–8329 117 [111] [0] 270 [insJ‑5]–[yieO] [insJ‑5],insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 3652533NA (NA)260 (1.130) 107/146 0.2 100% noncoding (1443/1443 nt) IS150 repeat region
?REL606 3901931 = 0 (0.000)coding (489/1428 nt) yieO predicted multidrug or homocysteine efflux system

Only 100 of 307 total aligned reads displayed.

GGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3652457‑3652533
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATGGCGACAAA  >  REL606/3901931‑3902005
                                                                                                                                                        
GGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTAC                                                                              >  2:3572529/1‑76
GGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTAC                                                                              <  2:3627963/76‑1
GGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTAC                                                                              <  1:4178377/76‑1
GGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTAC                                                                              <  1:6092965/76‑1
GGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTAC                                                                              <  1:4584356/76‑1
 GTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA                                                                             >  2:5322040/1‑76
 GTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA                                                                             >  2:6937129/1‑76
 GTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA                                                                             <  2:4540937/76‑1
 GTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA                                                                             <  1:5674705/76‑1
  TCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAG                                                                            >  1:1401271/1‑76
    TGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGT                                                                          <  2:5037368/76‑1
    TGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGT                                                                          <  2:736624/76‑1
    TGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGT                                                                          <  1:5378172/76‑1
     GACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTT                                                                         >  2:4289924/1‑76
     GACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTT                                                                         >  2:2488585/1‑76
       CTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGC                                                                       >  1:1295748/1‑76
         CCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCC                                                                     <  1:5886368/76‑1
          CAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCA                                                                    >  2:4391726/1‑76
          CAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCA                                                                    >  1:1832389/1‑76
           AATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAG                                                                   >  1:5717783/1‑76
            ATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGG                                                                  >  1:7576225/1‑76
              TGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTG                                                                <  2:6043248/76‑1
              TGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTG                                                                >  1:1788303/1‑76
              TGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTG                                                                >  1:5084848/1‑76
              TGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTG                                                                <  2:4002588/76‑1
              TGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTG                                                                <  2:6213376/76‑1
                AATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGAC                                                              >  2:1495246/1‑76
                 ATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACC                                                             <  1:3259637/76‑1
                  TATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCA                                                            <  1:3397397/76‑1
                  TATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCA                                                            <  1:6283482/76‑1
                  TATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCA                                                            >  1:1098263/1‑76
                   ATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAG                                                           >  1:6591721/1‑76
                    TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGC                                                          <  2:7176256/76‑1
                    TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGC                                                          <  2:4661657/76‑1
                     CGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCA                                                         <  1:1617554/76‑1
                        AATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGC                                                      <  1:2684864/76‑1
                        AATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGC                                                      <  2:3294365/76‑1
                         ATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCC                                                     <  1:1434948/76‑1
                            AGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCa                                                  >  2:6445894/1‑75
                            AGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCC                                                  >  2:4687811/1‑76
                             GACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCA                                                 <  2:6583716/76‑1
                              ACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAA                                                <  1:4830344/76‑1
                              ACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAA                                                <  2:2608620/76‑1
                              ACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAA                                                <  1:7137551/76‑1
                               CCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAG                                               <  1:6338295/76‑1
                               CCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAG                                               >  2:3074634/1‑76
                                CTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGA                                              >  2:2942912/1‑76
                                CTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGA                                              <  1:23012/76‑1
                                 TATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAA                                             <  1:3173664/76‑1
                                 TATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAA                                             >  1:4412528/1‑76
                                 TATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAA                                             >  1:7541172/1‑76
                                  ATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAAC                                            >  2:3042990/1‑76
                                  ATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAAC                                            <  2:799538/76‑1
                                  ATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAAC                                            >  1:2840487/1‑76
                                  ATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAAC                                            >  1:6651089/1‑76
                                    ATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGG                                          >  1:6540492/1‑76
                                       CCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGC                                       <  2:1046988/76‑1
                                        CTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCC                                      >  1:4485635/1‑76
                                         TCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCT                                     >  1:6098085/1‑76
                                          CGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTA                                    <  1:550976/76‑1
                                          CGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTA                                    >  1:3950085/1‑76
                                          CGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTA                                    <  2:4449720/76‑1
                                          CGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTA                                    >  1:6748168/1‑76
                                            TGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAA                                  >  2:5346586/1‑76
                                            TGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAA                                  >  2:3886871/1‑76
                                            TGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAA                                  >  1:4719834/1‑76
                                             GTTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAA                                 >  1:3870931/1‑76
                                              TTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAAT                                >  1:2593139/1‑76
                                              TTTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAAT                                >  1:7193148/1‑76
                                               TTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAACATT                               >  2:5648561/1‑76
                                               TTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATT                               >  2:5283880/1‑76
                                               TTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATT                               >  2:5143129/1‑76
                                               TTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATT                               >  1:2060742/1‑76
                                               TTAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATT                               >  2:4746369/1‑76
                                                TAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTG                              >  2:2006458/1‑76
                                                TAACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTG                              >  2:4581556/1‑76
                                                 AACTGTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGG                             <  1:3641680/76‑1
                                                    TGTCCAACTGTTTAGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCC                          <  1:6850639/76‑1
                                                     GTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCC                         >  1:959030/1‑76
                                                     GTCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCC                         <  1:2922814/76‑1
                                                      TCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCA                        <  2:6860141/76‑1
                                                      TCCAACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCA                        >  1:6440468/1‑76
                                                          ACTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAG                    <  1:6994523/76‑1
                                                           CTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGA                   <  2:2394909/76‑1
                                                           CTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGA                   <  2:129268/76‑1
                                                           CTTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGA                   <  2:5878435/76‑1
                                                            TTTTTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGAC                  >  2:3398070/1‑76
                                                               TTGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCG               <  2:6276690/76‑1
                                                                TGGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGG              >  1:6033954/1‑76
                                                                 GGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGC             <  2:5759316/76‑1
                                                                 GGGGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGC             >  1:5564243/1‑76
                                                                   GGTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCAT           <  2:2729221/76‑1
                                                                    GTCAGTACAGGTTGCCCAGGTGCCCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATG          <  2:2775051/76‑1
                                                                    GTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATG          <  2:7223562/76‑1
                                                                    GTCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATG          <  1:5805769/76‑1
                                                                     TCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATGG         >  1:1900687/1‑76
                                                                     TCAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATGG         <  1:742602/76‑1
                                                                      CAGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATGGC        >  2:2668551/1‑76
                                                                       AGTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATGGCG       <  1:2493248/76‑1
                                                                        GTACAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATGGCGA      <  1:3015732/76‑1
                                                                                                                                                        
GGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3652457‑3652533
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATGGCGACAAA  >  REL606/3901931‑3902005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.