| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,376,348 | 0 | T | G | 38.3% | 255.3 / 38.0 | 150 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| Reads supporting (aligned to +/- strand): ref base T (22/70); new base G (56/1); total (78/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.39e-21 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 200 total aligned reads displayed.
GCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376273‑2376423 | aCCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggggg > 1:3186366/1‑76 (MQ=255) ccAGATGAGGCGACCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtggt < 2:6032305/76‑1 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtggt < 1:4426839/76‑1 (MQ=255) cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtgggg > 2:2608923/1‑74 (MQ=255) aGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGc > 2:5608392/1‑76 (MQ=255) aGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGc > 1:7239851/1‑76 (MQ=255) aTGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCt > 1:1135401/1‑76 (MQ=255) aTGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGCGGGGCCt > 2:1246859/1‑76 (MQ=255) gAGGCGAACTGTAGCCGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 1:2899365/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 2:1561033/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 1:924914/76‑1 (MQ=255) gAGGCGAACTGGAGACGCCCCCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 1:3488760/76‑1 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTg < 1:5157335/76‑1 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTg < 2:1566345/76‑1 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTg < 1:1049520/76‑1 (MQ=255) ctaaCTGTAGACGCCGCCTTTGTACCCCGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCa < 2:4536576/74‑1 (MQ=255) cGACCTTTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCa < 2:2465376/76‑1 (MQ=255) cGACCTGGAGCCGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCa < 2:4754422/76‑1 (MQ=255) cGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGGCTTTGCCa > 1:5268905/1‑76 (MQ=255) cGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGccc > 1:7576884/1‑75 (MQ=255) cGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGGCa > 1:743523/1‑76 (MQ=255) aCTGTAGACGCCCTCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTc < 1:2614929/76‑1 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTc < 2:6972877/76‑1 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGGCATTc > 1:5614089/1‑76 (MQ=255) tGTAGACGCCCGCTTTTCTCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGt < 2:4596206/76‑1 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGCCCTTCGt > 1:6688432/1‑76 (MQ=255) tggACCCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAc < 2:6837105/74‑1 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGGCCTTTCCAATCGCAc > 1:7310239/1‑76 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAcc > 2:5723765/1‑76 (MQ=255) gACCCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAccc < 2:890853/76‑1 (MQ=255) gcccccGCTTTGTACCCTGCCGCCATCAAAATCGACGCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCg < 1:2349857/73‑1 (MQ=255) ccGCCCTCTTTTAACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCg < 2:3825108/75‑1 (MQ=255) aCGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCg > 2:6740775/1‑76 (MQ=255) aCGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCg > 2:1768410/1‑76 (MQ=255) gCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCa < 1:5090371/76‑1 (MQ=255) gCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTAACCGCa > 1:2844343/1‑76 (MQ=255) cccccCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCa < 2:1424330/75‑1 (MQ=255) ccACTTGGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAAc < 1:1648506/76‑1 (MQ=255) aCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACACGCAACgg > 2:1478352/1‑76 (MQ=255) tttGTCCCCTGCCGCCATCAGAATCGACCCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTa < 2:4137535/76‑1 (MQ=255) tttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCCTTGCCATTCGAACCCGCCACggga > 1:5534669/1‑74 (MQ=255) ttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGGaa > 2:30272/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTaaa > 1:6512984/1‑76 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGGaaaa > 2:2484486/1‑75 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGACTTTTTCCTTCGTACCCGCAACCGCAAac > 2:6930609/1‑76 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGGCAATCGTACCCGCCACGGGaaaa > 2:4848435/1‑75 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGGAAacac > 2:6229366/1‑76 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaa < 1:3773224/76‑1 (MQ=255) cggccgccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaa < 1:5853369/74‑1 (MQ=255) cTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAACCGCAACGGGAAACACaaa > 2:1128566/1‑76 (MQ=255) cTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGCGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaa < 2:1290116/76‑1 (MQ=255) tcccgccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 2:1957610/74‑1 (MQ=255) tGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 1:5043885/76‑1 (MQ=255) tGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaaa > 1:4047047/1‑75 (MQ=255) tGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGGCATTCGTAACCGCCACGGTAAACACaaaa > 2:2969722/1‑75 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGGATTTCGTTCCCGCAACGGTAAAAACAAATg > 1:5752219/1‑76 (MQ=255) cccgccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATg < 2:372608/75‑1 (MQ=255) ccgccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATgg < 2:2304419/76‑1 (MQ=255) cgccATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCCTTGGCAATCGTAGACGCAAACGTAAACACAAATGgc > 2:3533153/1‑76 (MQ=255) gccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGgcg > 1:2577528/1‑76 (MQ=255) ccATCAGAATCGACTCCAGCGTACGGCAGGGGGGGTCTTTGCCATTCGTACCCGCAACGGGAAAAACAAATGgggc > 1:736310/1‑76 (MQ=255) ccATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAACCCAAATGgcgc > 1:6396895/1‑76 (MQ=255) caccAGAATCGACTCCAGCGTACGGCAGGTGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCt < 1:3245529/73‑1 (MQ=255) cATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGGAAACACAAATGGCGCt > 1:4625779/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTg > 1:1404479/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCCCAACGGTAAACACCAATGGGGCTg > 1:6995920/1‑76 (MQ=255) tCAGAATCGACTCCAGCGTACGGCAGGGGGTGGCTTTGCCATTCGTCCCCGCAACGGTAAACACAAAAGGCGCTgg > 2:5238079/1‑76 (MQ=255) tCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGGAAACACAAATGGCGCTgg > 2:975888/1‑76 (MQ=255) cAGAATCGACTCCAGCGTACGGCAGGTGGGGTCTTTGCCATTAGTACCCGCAACGGTAAACACAAAAGGCGCCGGt > 2:5530178/1‑76 (MQ=255) cAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGt > 1:3344217/1‑76 (MQ=255) aGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTGCCCTTCGTACCCGCAACGGTAAACACAAATGGCGCTGGtt > 2:3217585/1‑76 (MQ=255) aGAATCGACCCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGtt < 1:3114383/76‑1 (MQ=255) gAATCGCCTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt < 1:5225701/76‑1 (MQ=255) gAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt < 2:737259/76‑1 (MQ=255) aaTCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTc < 2:4418372/76‑1 (MQ=255) agcGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCa < 1:2431904/74‑1 (MQ=255) aTCGACTCCAGCGTACGGCAGGGGGGGCCTTTGGCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGgttta > 1:5949800/1‑74 (MQ=255) tCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAg < 2:506524/76‑1 (MQ=255) tCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAg < 2:4217069/76‑1 (MQ=255) tCGACTCCAGCGTACGGCAGGGGGGGCCTTTGACATTCGTACCCCCCACGGTAAACACAAATGGCGCTGGGTTCAg > 1:6649842/1‑76 (MQ=255) cGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 1:1486141/76‑1 (MQ=255) cGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCCACGGGAAACACAAAAGGCGCTGGGTTCAgg > 1:894243/1‑76 (MQ=255) gACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGa > 1:2260365/1‑76 (MQ=255) aCTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGAc < 1:6064542/76‑1 (MQ=255) cTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACg > 2:6067577/1‑76 (MQ=255) tCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGc < 1:3265194/76‑1 (MQ=255) ccAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGcc < 1:4877871/76‑1 (MQ=255) ccAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGcc < 2:7444187/76‑1 (MQ=255) cgtcGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCa < 1:7509630/73‑1 (MQ=255) aGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCaa < 1:2534935/76‑1 (MQ=255) gCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 1:2735146/76‑1 (MQ=255) gCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 1:3859271/76‑1 (MQ=255) gCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 2:7178738/76‑1 (MQ=255) gTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGGTTCAGGACGCCAAGAc > 2:2334096/1‑76 (MQ=255) aGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 1:1906755/1‑76 (MQ=255) aGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 2:4329055/1‑76 (MQ=255) aGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 2:1156308/1‑76 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg > 1:993071/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 1:7154327/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 1:7210752/1‑76 (MQ=255) | GCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376273‑2376423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |