| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,441,705 | 0 | T | G | 42.6% | 108.6 / 10.4 | 68 | T139P (ACC→CCC) | ypdG | predicted enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base T (28/11); new base G (29/0); total (57/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.63e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 131 total aligned reads displayed.
AGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAA > REL606/2441630‑2441780 | ggTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTa < 1:4302884/76‑1 (MQ=255) gTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAg < 1:425396/76‑1 (MQ=255) gTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAg < 1:3464655/76‑1 (MQ=255) gTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAg > 1:2239301/1‑76 (MQ=255) gTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAg > 1:3167736/1‑76 (MQ=255) tGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGgaga > 2:4756388/1‑76 (MQ=255) tGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGgaga > 1:4123089/1‑76 (MQ=255) gACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACa < 1:551533/76‑1 (MQ=255) cGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATc < 2:5012634/76‑1 (MQ=255) cGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATc < 1:583955/76‑1 (MQ=255) cGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATc < 1:2228440/76‑1 (MQ=255) ggCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCa < 1:2401349/76‑1 (MQ=255) ggCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGGCAtaa > 2:3342567/1‑74 (MQ=255) ggCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCa > 2:5863015/1‑76 (MQ=255) gCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCaa < 2:4225442/76‑1 (MQ=255) cACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAAc > 1:7080011/1‑76 (MQ=255) aCCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCaaac > 1:2773390/1‑74 (MQ=255) aCCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAAcc > 1:1150508/1‑76 (MQ=255) aCCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGAAATaaaac > 2:7428186/1‑74 (MQ=255) aCCGGCAAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCaaac > 2:2002119/1‑74 (MQ=255) cGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAAcccc > 2:6722124/1‑75 (MQ=255) cGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAAcccc > 2:7011338/1‑75 (MQ=255) ggCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGCGAAATCAAAccgc > 2:171070/1‑76 (MQ=255) ggAAGAGGTCGCCCATTGCATAGTGCGGGGATCGAAGTCCAGCAGTGGCGTCGGTTGGGTAGACATCACCccgccg < 2:3452867/75‑1 (MQ=255) cGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGGGACATCAAAcccccc > 1:4865642/1‑75 (MQ=255) cGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGGCATCAAAcccccc > 2:4045099/1‑75 (MQ=255) cGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACccgccg > 1:1326634/1‑76 (MQ=255) cGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATAAAAccgccg > 1:1354708/1‑76 (MQ=255) gAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCCCCGa > 2:7370854/1‑76 (MQ=255) gAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGa > 2:288057/1‑76 (MQ=255) gAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGa > 1:3554020/1‑76 (MQ=255) aaGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACccgccgct > 1:2401978/1‑74 (MQ=255) aaGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGAt > 1:6571446/1‑76 (MQ=255) gagGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATaa > 1:2414964/1‑76 (MQ=255) ggTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATaaaa > 1:1916922/1‑76 (MQ=255) ggTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCCATaaaa > 2:1921712/1‑76 (MQ=255) ggTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCCCCGATaaaa > 1:2976400/1‑76 (MQ=255) gTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGGGACATCAACCCGCCGCGAAAAc > 2:4027027/1‑76 (MQ=255) gCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGGTAAAACCAg > 2:1307627/1‑76 (MQ=255) cccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGATATCAAACCGCCGAGAAAACCacc > 2:5451326/1‑74 (MQ=255) cccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCCGc > 2:7290422/1‑76 (MQ=255) cccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGc > 1:858863/1‑76 (MQ=255) cccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCCCCGATAAAACCAGc > 2:1129552/1‑76 (MQ=255) cccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCAGGGTAGACATCAACCCGCCGATAAAACTAGc > 2:3806852/1‑76 (MQ=255) ccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAAACAGcc > 2:5800871/1‑76 (MQ=255) ccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAaaccaacc > 1:996934/1‑76 (MQ=255) cATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATTAACCCGCCCATAAAACCAGCCg > 2:2651983/1‑76 (MQ=255) cATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGCTAAAAACAGCCg > 1:2541450/1‑76 (MQ=255) aTTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGGTAAAACCAGCCgg > 1:3397778/1‑76 (MQ=255) aTTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAAACAGCCgg > 1:6541229/1‑76 (MQ=255) aTTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCCCCGATAAAACCAGCCgg > 1:4003791/1‑76 (MQ=255) ttGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAAACCGCCGATAAAAACAGCCggg > 1:3042648/1‑76 (MQ=255) cATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAAc > 1:4997665/1‑76 (MQ=255) cATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAAACCGCCGATAAAAACAACCGGGGAc > 2:5181331/1‑76 (MQ=255) aTAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCCCCGATAAAACCAGCCGGGAACg > 1:3625071/1‑76 (MQ=255) aTAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCCCCGATAAAAACAGCCGGGaaac > 1:1567578/1‑74 (MQ=255) tAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCCACCCGCCGCTAAAACCAGCCGGGAACGc > 1:3114095/1‑76 (MQ=255) aGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGcc > 2:717542/1‑76 (MQ=255) aGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAAACCGCCGAAAAAAACAGCCGCGAACGcc > 2:3499642/1‑76 (MQ=255) gTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCa > 1:4443792/1‑76 (MQ=255) tGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAg > 1:6775199/1‑76 (MQ=255) cTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGtt > 1:7398481/1‑76 (MQ=255) tGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAAACAGCCGGGAACGCCAGttt > 1:3241394/1‑76 (MQ=255) gggATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAAACAGCCGGGAACGCCAGGTTg > 2:3825000/1‑76 (MQ=255) gggATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAAACCCCCGATAAAACCAGCCGGGGAAGCCAGTTTg > 2:4145301/1‑76 (MQ=255) ggATCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAAAGCCAGttttc > 1:261583/1‑74 (MQ=255) aTCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGccc > 2:2812542/1‑75 (MQ=255) tCGAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGGACGCCAGTTTGGCAc > 1:1527943/1‑76 (MQ=255) gAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCg > 1:5324733/1‑76 (MQ=255) gAAGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAAACCGCCGCTTAAAACAGCCGGGAACGCCAGTTTGCCCCCg > 2:3332583/1‑76 (MQ=255) aaGTTCAGCAGTTGCGTCGGCTGGGGAGAACACAACCCGCCGATAAAACCAACCGGGAAAGCCAAGTTTCCAccca > 2:5362738/1‑74 (MQ=255) aGTTCAGCAGTTGCGTCGGCTGGGGAGACATCAAACCGCCGGTAAAACCAGCCGGGAACGCCAGTTTGCCACCGaa > 2:465191/1‑75 (MQ=255) ttCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATag > 2:6860783/1‑76 (MQ=255) ttCAGCAGTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAAACAACCGGGAAAGCCAGGTTGCCCCCCATag > 2:6034780/1‑76 (MQ=255) cagcagTTGCGTCGGCTGGGGAGACATCAAACCGCCGATAAAACCAGCCCGGAACGCCAGGTTGCCCCCCACagag > 2:1179173/1‑76 (MQ=255) agcagTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGt > 2:1518678/1‑76 (MQ=255) agcagTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGAAAGAGt > 1:1512224/1‑76 (MQ=255) agTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGGTTGCCACCGATAGAGTTCg > 1:1223434/1‑76 (MQ=255) agTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCg > 2:638571/1‑76 (MQ=255) agTTGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGGTCg > 2:5930941/1‑76 (MQ=255) ttGCGTCGGCTGGGGAGACAACAAACCGCCGATAAAACCAGCCGGGAACGCCAGCTTGGCACCGATAGAGTTCGcc > 2:4834705/1‑76 (MQ=255) tGCGTCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCCGTTTGCCCCCCATAAAGTTCGccc > 2:5005999/1‑75 (MQ=255) gTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGGTCGCCACaa > 1:6426434/1‑76 (MQ=255) tCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaa > 1:7383153/1‑76 (MQ=255) tCGGCTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGGTCGCCACaaa > 2:4949817/1‑76 (MQ=255) cGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa > 2:3074834/1‑76 (MQ=255) cGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa > 1:2783924/1‑76 (MQ=255) cGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa > 1:683765/1‑76 (MQ=255) cGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa > 1:7644074/1‑76 (MQ=255) cGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAAAGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa > 1:557486/1‑76 (MQ=255) ggCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAag > 1:5052824/1‑76 (MQ=255) cTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACACAagca > 1:4621043/1‑76 (MQ=255) cTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagca > 2:1651331/1‑76 (MQ=255) tGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagcag > 1:7400600/1‑76 (MQ=255) tGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagcag > 2:1640015/1‑76 (MQ=255) tGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagcag > 2:1923921/1‑76 (MQ=255) gggTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagcagc > 2:2663151/1‑76 (MQ=255) gggTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagcagc > 1:395735/1‑76 (MQ=255) gTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaa > 2:7534736/1‑76 (MQ=255) tAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaaa > 2:2672664/1‑76 (MQ=255) | AGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAA > REL606/2441630‑2441780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |