Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,515,9090AC50.6% 74.2 / 128.0 79V260G (GTG→GGG) ypfIpredicted hydrolase
Reads supporting (aligned to +/- strand):  ref base A (25/14);  new base C (0/40);  total (25/54)
Fisher's exact test for biased strand distribution p-value = 6.04e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-11
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 118 total aligned reads displayed.

GTTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATT  >  REL606/2515834‑2515984
                                                                              |                                                                            
gTTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGacca                                                                              <  2:2767986/76‑1 (MQ=255)
  tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGaccacc                                                                            <  1:1279139/76‑1 (MQ=255)
  tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGaccacc                                                                            <  2:6562802/76‑1 (MQ=255)
  tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGaccacc                                                                            <  2:5109532/76‑1 (MQ=255)
   aacaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGGGGGTTTCTTGGaccacc                                                                            >  1:6534833/1‑76 (MQ=255)
   aacaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGAtcacca                                                                           <  1:3815612/76‑1 (MQ=255)
   aacaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGAccacca                                                                           <  1:1558443/76‑1 (MQ=255)
   aacaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGAccacca                                                                           <  2:6008452/76‑1 (MQ=255)
   aacaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGAccacca                                                                           <  2:3514245/76‑1 (MQ=255)
   aacaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGAccacca                                                                           <  1:271844/76‑1 (MQ=255)
    acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccag                                                                          <  2:7257654/76‑1 (MQ=255)
    acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccag                                                                          <  2:246304/76‑1 (MQ=255)
       acGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccagcca                                                                       <  1:1793432/76‑1 (MQ=255)
       acGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccagcca                                                                       <  2:1257859/76‑1 (MQ=255)
       acGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccagcca                                                                       <  1:525755/76‑1 (MQ=255)
       acGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccagcca                                                                       <  1:4940208/76‑1 (MQ=255)
        cGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccagccag                                                                      <  1:3153482/76‑1 (MQ=255)
        cGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccagccag                                                                      <  2:4619796/76‑1 (MQ=255)
        cGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccagccag                                                                      <  2:3802231/76‑1 (MQ=255)
           tCGAGAAAAACGCGATCCCATTTGATGAAACAAGGGCGCAGGTATGGCTGCGGCTTCATCGCCCCCCAGCCAGTCg                                                                  <  2:5304978/76‑1 (MQ=255)
             gAGGAAACCGCGATCCCATTTGATCCAAAAAGGGCGCAGGTATGGCTCCGGCTTCATCGCCCCCCAGCCAGTCGGc                                                                <  2:1841425/76‑1 (MQ=255)
             gAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGGGGGCTTCTTGGCCACCACCCCGTCGGc                                                                >  2:4932592/1‑76 (MQ=255)
             gAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGGGGGTTCTTACACCACCAGCCCGTGGGc                                                                >  1:5570760/1‑76 (MQ=255)
               taaaagCGCGATATCAGTTGACGCAACAAGGAGGGAGGTTGGTTTGCGGCTTCACCGCCCTCCAGCCAGTCGGCtt                                                              <  2:1630192/74‑1 (MQ=255)
               ggAAAACGCGATACCAGTGGATGCAAAAATGGCGCAGGTATGGCTGCGGCTTCATCGACCCCCAGCCAGTCGGCtt                                                              <  2:7179246/76‑1 (MQ=255)
               gaaaaaCGCGATCCCAGTTGATGCAACAAGGGCGCGGGTATGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCtt                                                              <  2:2741565/74‑1 (MQ=255)
               agAAAACGCGATTCCAGTTAATGAAACAATGGCGCAGGTATGGCTGGGGCTTCATCGCCCCCCAGCCAGTCGGCtt                                                              <  2:1889066/75‑1 (MQ=255)
                        gATCCCATTTGATGCAACAATGGCGCAGGTATGGCGGCGGCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGc                                                     <  2:3290755/76‑1 (MQ=255)
                        gATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTTATCGACCCCCCACCAGGCGGGCTTGTCATCGc                                                     >  1:2214677/1‑76 (MQ=255)
                         atcccAGTTGAGGCAACAAGGGCGCAGGTATGGCTGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCt                                                    <  1:4130497/73‑1 (MQ=255)
                          tcccAGTTGATGCAACAAGGGCGCAGGTATGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTg                                                   <  1:2016408/74‑1 (MQ=255)
                          tACCAGTTGATGCAACAATGGCGAAGGTATGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTg                                                   <  2:5373159/76‑1 (MQ=255)
                             cggTTGATGAAACAATGGCAGAGTTATGCTTGCGGCTTCCCCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCt                                                <  2:4793046/74‑1 (MQ=255)
                             cAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCt                                                <  1:7271806/76‑1 (MQ=255)
                              aGTTGAGACAACACTGGCGAAGGTTGGGCTGCGCCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTa                                               <  2:1600300/76‑1 (MQ=255)
                              aGTGGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTa                                               <  1:4161678/76‑1 (MQ=255)
                               gTTGATGCAACAATGGCGCAGTTATGGCTGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTaa                                              <  1:6314644/76‑1 (MQ=255)
                               gTTGATGCAACAAGGGCGAGGGTAGGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTaa                                              <  2:2280051/76‑1 (MQ=255)
                               gTTGAGGCAAGAATGGCGCAGTTCGGCCGGGGACTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTaa                                              <  2:6917342/76‑1 (MQ=255)
                               gTTGACGCAACAGGGGCGGAGGTAGGGCGGCGTCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTaa                                              <  1:2348784/76‑1 (MQ=255)
                                ttGATGCAACAATGGGGCGGGTATGGCTGCGCCTTCACCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAAc                                             <  1:1845953/76‑1 (MQ=255)
                                 tggtGCAAAAATGGCGGGGGTTTGGCTGGGGCTTCATCCCCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAAc                                             <  1:6240309/73‑1 (MQ=255)
                                 tGATGCAACAAGGGCGCAGGTATGGCTGCGCCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGTTAACa                                            <  2:3767950/76‑1 (MQ=255)
                                 tGATGCAAAATGGGCGCCGGTATGGCTGCGCCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACa                                            <  2:5186422/76‑1 (MQ=255)
                                 ggATGAAACAAGGGCGCGGGTATGGCTGCGCCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACa                                            <  2:4731681/75‑1 (MQ=255)
                                  tgtGCAAAATTGGCGCGGTTTGGGCTGGGCTTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACaa                                           <  2:5017029/74‑1 (MQ=255)
                                  gATGCAACAATGGGGCGGGTATGTCTGCGTTTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACaa                                           <  1:4211230/76‑1 (MQ=255)
                                  gATCCAACAGGGGCGCGGGTTTGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACaa                                           <  1:7053440/76‑1 (MQ=255)
                                    tGCAACAATGGCGCGGGTAGGGCGGCGGTTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAgg                                         <  1:4940655/76‑1 (MQ=255)
                                     gCAACAAGGGCGCAGGTAGGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGc                                        <  1:4378244/76‑1 (MQ=255)
                                      caacaaGGGCGCAGTTAGGGCTGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCa                                       <  2:2950382/76‑1 (MQ=255)
                                           gtGGCGCAGTTATGGCGGCGGTTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCgg                                  <  2:823237/75‑1 (MQ=255)
                                             ggCGCGGGTTGGCCTGCGGCTCCATGGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGgcg                                <  2:1118463/76‑1 (MQ=255)
                                                gcgggTGTGGCGGCGCCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCaa                             <  1:117175/73‑1 (MQ=255)
                                                  gggTATGGCGGCGGCTTCACCGCCCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATa                           <  2:1721348/75‑1 (MQ=255)
                                                   ggTATGGCTGCGTCTTCATCCACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATaa                          <  1:7422072/76‑1 (MQ=255)
                                                    gTATGGCGCGGCCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATaaa                         <  1:1564688/76‑1 (MQ=255)
                                                     tgtGGCGGCGGTTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAg                        <  1:3343747/74‑1 (MQ=255)
                                                     tATGCCTGCGCCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAg                        <  2:4169127/76‑1 (MQ=255)
                                                      aTGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGGTCATCGCTGGGTAACAAGGCATCCGGCGCCATAAAGc                       >  1:2131395/1‑76 (MQ=255)
                                                       tGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTTCTCGCTGGGTAACAAAGGAACCCGCGCCATAAAACg                      >  2:3418736/1‑76 (MQ=255)
                                                       tGGCTGCGGCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCg                      <  2:2026764/76‑1 (MQ=255)
                                                        ggCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGGGCCAAAAAGCGa                     >  1:3862388/1‑76 (MQ=255)
                                                        ggCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAAGCATCCGGGGCAAAAAAGCGa                     >  2:2871743/1‑76 (MQ=255)
                                                        ggCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAAGCATCCGGCGCCATAAAGCGa                     >  1:3179257/1‑76 (MQ=255)
                                                         gCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaa                    >  1:3325796/1‑76 (MQ=255)
                                                         gCTGCGGCTCCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaa                    <  1:6191136/76‑1 (MQ=255)
                                                         gCTGCGCCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaa                    <  1:4820751/76‑1 (MQ=255)
                                                         gCTGCGCCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaa                    <  1:2418786/76‑1 (MQ=255)
                                                          cTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaaa                   >  1:2678813/1‑76 (MQ=255)
                                                          cTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAACGaaa                   >  1:4113016/1‑76 (MQ=255)
                                                          cTGCGCCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaaa                   <  1:7165797/76‑1 (MQ=255)
                                                          cTGCGCCTCCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaaa                   <  2:3156831/76‑1 (MQ=255)
                                                           tGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCCACCGGAGCAACAAAGCCaaaa                  >  1:7466019/1‑75 (MQ=255)
                                                           cggCGGTTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaaa                   <  2:1532302/74‑1 (MQ=255)
                                                            ggcgcCTGCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAAc                  <  1:2085743/75‑1 (MQ=255)
                                                             gcagCTTCATCCCCCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACt                 <  2:1570650/73‑1 (MQ=255)
                                                              cGGCTTTATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAAAAAAGCATCCGGCGCCATTAAGCGAAACtt                >  2:5601353/1‑76 (MQ=255)
                                                               ggCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAACGAAACTtc               >  2:4483277/1‑76 (MQ=255)
                                                                gCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtct              >  2:7257918/1‑76 (MQ=255)
                                                                 ccTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtct              <  1:210636/75‑1 (MQ=255)
                                                                  tttCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtctc             <  1:2060631/75‑1 (MQ=255)
                                                                  cTTCATCGCCCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtctc             <  2:1012985/76‑1 (MQ=255)
                                                                   ttCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCCATAAAGCGAAACTtctcc            >  2:1615892/1‑75 (MQ=255)
                                                                   ttCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGCAACTTCTCg            >  2:3302681/1‑76 (MQ=255)
                                                                   ttCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg            >  2:1580636/1‑76 (MQ=255)
                                                                   ttCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg            >  2:3070274/1‑76 (MQ=255)
                                                                   ttCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg            >  1:4822704/1‑76 (MQ=255)
                                                                   ttCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg            >  1:3060052/1‑76 (MQ=255)
                                                                    tCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGc           <  2:980461/76‑1 (MQ=255)
                                                                     cATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACCAGGCATCCCGCGCAATAAAGCGAAACTTCTCGcc          >  2:6168822/1‑76 (MQ=255)
                                                                     cATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGcc          >  1:5433274/1‑76 (MQ=255)
                                                                      aTCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGccc         >  2:2142919/1‑76 (MQ=255)
                                                                      aTCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGccc         >  2:2040455/1‑76 (MQ=255)
                                                                       tCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCg        <  1:7379918/76‑1 (MQ=255)
                                                                          gACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCa      >  2:3800596/1‑76 (MQ=255)
                                                                          gACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCa      >  2:6115066/1‑76 (MQ=255)
                                                                           accaccAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCaa     >  2:818879/1‑76 (MQ=255)
                                                                              accaGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAAtt  >  2:4354890/1‑76 (MQ=255)
                                                                              accaGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAAtt  >  2:2948366/1‑76 (MQ=255)
                                                                              |                                                                            
GTTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATT  >  REL606/2515834‑2515984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: