| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,676,636 | 0 | T | G | 41.3% | 401.3 / 102.8 | 244 | V149G (GTG→GGG) | smpB | SsrA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (13/129); new base G (100/0); total (115/129) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 280 total aligned reads displayed.
AAGTGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCC > REL606/2676561‑2676711 | aaGTGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGt < 1:4725113/76‑1 (MQ=255) gTGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGgggg > 2:2113231/1‑76 (MQ=255) gTGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTgg < 2:7228098/76‑1 (MQ=255) gTGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTgg < 1:251879/76‑1 (MQ=255) tGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGa < 1:2233110/76‑1 (MQ=255) gAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGgggggt > 2:6692792/1‑74 (MQ=255) gAAAAGCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGAt < 2:3839654/76‑1 (MQ=255) aaaaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATa < 2:2289112/76‑1 (MQ=255) aaaaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTa > 2:1037314/1‑76 (MQ=255) aaaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATaa < 2:5967670/76‑1 (MQ=255) aaaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATaa < 2:544519/76‑1 (MQ=255) aaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATaaa < 1:5113739/76‑1 (MQ=255) aaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATaaa < 1:924737/76‑1 (MQ=255) aaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTaaa > 1:7411374/1‑76 (MQ=255) aaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAACGGCAGGGGGGTaaa > 2:3661033/1‑76 (MQ=255) agcGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAg < 1:2789783/74‑1 (MQ=255) aTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAg < 2:2037193/76‑1 (MQ=255) tCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGc < 1:569093/76‑1 (MQ=255) tCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGc < 2:7126136/76‑1 (MQ=255) tCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGc < 1:4538166/76‑1 (MQ=255) cGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCa < 1:3911661/76‑1 (MQ=255) ggCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCAc < 1:5498903/76‑1 (MQ=255) ggCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAACaa > 2:4193273/1‑75 (MQ=255) gCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACg < 1:7261581/76‑1 (MQ=255) gCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACg < 1:6974421/76‑1 (MQ=255) gCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACg < 1:6625724/76‑1 (MQ=255) cGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGt < 2:3075544/76‑1 (MQ=255) gTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTa < 1:6002979/76‑1 (MQ=255) cGCCAAAGGTAATAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGGATc > 1:2447116/1‑76 (MQ=255) gCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCa < 2:5210729/76‑1 (MQ=255) gCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCa > 1:7552716/1‑76 (MQ=255) gCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGTATCa > 2:7454087/1‑76 (MQ=255) ccAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGAACGTATCaa > 2:5409773/1‑75 (MQ=255) cAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATg < 1:1820967/76‑1 (MQ=255) cAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGGACGTATCATg > 2:2489167/1‑76 (MQ=255) cAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGAGGATAAAGCACGTATCATg > 2:1912949/1‑76 (MQ=255) aaaGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGa < 1:4453895/76‑1 (MQ=255) aaGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGaa < 2:4349685/76‑1 (MQ=255) aaGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAACACCGATCATCaa > 2:838529/1‑76 (MQ=255) ggTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGaaaa < 2:265234/76‑1 (MQ=255) ggTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCATGaaaa > 1:6443709/1‑76 (MQ=255) tAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAAc < 2:2778597/76‑1 (MQ=255) tAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGGATCATGaaaaaa > 1:5764182/1‑75 (MQ=255) tAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAACACGTATCATGaaaaaa > 2:5510455/1‑75 (MQ=255) tAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGAGAAAGCACGTATTATGAAAAAc > 1:4876476/1‑76 (MQ=255) aaGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATTAAGCACGCATCATTAAAAAcc > 2:6385512/1‑75 (MQ=255) aaGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGGAAAACACGTATTATGAAAAACg > 1:7183117/1‑76 (MQ=255) aGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAACACGGATCATGAAAAACGc > 2:3224112/1‑76 (MQ=255) gAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGcc < 1:780563/76‑1 (MQ=255) aaCAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCa < 1:3318022/76‑1 (MQ=255) aaCAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCa < 2:5878160/76‑1 (MQ=255) cAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCATGAAAAAAACCCAcc > 1:3313393/1‑76 (MQ=255) cAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAACACGGATCATGAAAAAAGcccccc > 1:1240263/1‑76 (MQ=255) cAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGTATCATGAAAAAAGcccccc > 2:4717124/1‑76 (MQ=255) gCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCATGAAAAAAGCCCACCgc > 2:6315338/1‑75 (MQ=255) cACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAACCGCCCACCGtt < 2:1641958/76‑1 (MQ=255) aCGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCATGAAAAACGCCCACCGTTa > 2:7542282/1‑76 (MQ=255) gATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTaaa < 1:3999792/76‑1 (MQ=255) gATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTaaa < 1:5194021/76‑1 (MQ=255) gATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCATGAAAAAAGCCCACCCTTaaa > 1:6593825/1‑76 (MQ=255) gATAAACGTTAAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATAAAAACCCCCCACCGTTaaa < 2:5494438/76‑1 (MQ=255) aTAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAAc < 1:1577161/76‑1 (MQ=255) aaaCGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCATGAAAAAAGCCCACCGTTAAACCt > 2:3201440/1‑76 (MQ=255) aaaCGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGTATTATCAAAAACGCCCCCCGTTAAACCt > 2:6575177/1‑76 (MQ=255) aaCGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTg > 2:5575278/1‑76 (MQ=255) aCGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGc < 1:7360597/76‑1 (MQ=255) aCGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGAGTAAACACGGAACATGAAAAACAAACACCGCTAAACCTGc > 2:3445674/1‑76 (MQ=255) cGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGGTAAAGCACGTATCATGAAAAACACCCACCGTTAAACCTGCa > 2:1397470/1‑76 (MQ=255) gTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCAc > 2:4010890/1‑76 (MQ=255) gTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGGACGTATTATGAAAAAACCCCACCGGTAAAACAGCAc > 2:6505100/1‑76 (MQ=255) ttCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACCAATCCTGAAAAAAGCCCCCCGTTAAAACTGccac > 2:1320667/1‑73 (MQ=255) tCAGATATGAGAGAGCCCGAATGGCAGGTGAATAAAGCAGGTATCATGAAAAACTCCCACCGTTAAACCTGCACTc < 2:6979199/76‑1 (MQ=255) cAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAAACTGCACTcc > 2:5816633/1‑76 (MQ=255) cAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGAACGTATCATGAAAAACGCCCAACGTTAAACCTGCACTcc > 1:4165457/1‑76 (MQ=255) cAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAACACGTATCCTGAAAAACGCCCACCGTTAAACCTGCACTcc > 2:6017097/1‑76 (MQ=255) gATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCaa < 1:1037314/76‑1 (MQ=255) gATATCAAAGAGCGCAAATGGCGGGTGGATAAAGCACGTATCATGAAAACCGCCCACCGTTAAACCTGCACTCCag < 2:1116049/76‑2 (MQ=255) atatCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAat < 1:4063996/76‑1 (MQ=255) atatCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCATGAAAAACGCCCCCCGTTAAACCTGCACTCCAat > 1:3811161/1‑76 (MQ=255) tatCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGTATCATGAAAAACGCCCCCCGTTAAACCTGCACTCCAatt > 2:5080505/1‑76 (MQ=255) tCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAattat < 1:170381/76‑1 (MQ=255) cAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCATGAAAAAAGCCCACCGGTAAAACTGCACTCCAattatt > 1:2947611/1‑76 (MQ=255) aaGAGCGCGAATGGCAGGGGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGa > 1:5489401/1‑76 (MQ=255) agagCGCCAATGGCAGGTGTAAAAAGCCCGTATCATGAAAACCGCCCACCGTTAAACCTTCACTCCAATTATTGAc < 1:1931282/76‑1 (MQ=255) agCGCGAATGGCAGGGGGGTAAAAACCGTATTATGAAAAAAACCCACCGTTAAACCTGCACTCCCATTATTTAccc > 2:6852129/1‑75 (MQ=255) gcgAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGtt < 2:6097502/76‑1 (MQ=255) gAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTcc < 2:503857/76‑1 (MQ=255) gAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTcc < 2:2874025/76‑1 (MQ=255) gAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTcc < 1:3150319/76‑1 (MQ=255) aaTGGCAGGTGGATAAAGCACGTATCATGAAAACCGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCt < 2:2367445/76‑1 (MQ=255) aaTGCCAGGTGGATAAAGCACGTATCAGGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCt < 1:2083261/76‑1 (MQ=255) aTGGCAGGTGGATAAAGCACGTATCATGAAAACCGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTc < 1:6781178/76‑1 (MQ=255) aTGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTc < 2:4838049/76‑1 (MQ=255) aTGGCAGGGGGGTAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTTACCAGTTCCTc > 2:2199465/1‑76 (MQ=255) tGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCa > 2:4474374/1‑76 (MQ=255) ggCAGGTGGATAAAGCACTTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCAc < 1:7075495/76‑1 (MQ=255) ggCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCAc < 1:5423196/76‑1 (MQ=255) ggCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCAc < 1:3522552/76‑1 (MQ=255) cAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACcg > 1:1281730/1‑76 (MQ=255) aGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACcgc < 1:1378403/76‑1 (MQ=255) | AAGTGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCC > REL606/2676561‑2676711 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |