| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 931,314 | 0 | T | C | 40.3% | 117.6 / 59.2 | 119 | E84G (GAG→GGG) | ybjE | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (35/36); new base C (1/47); total (36/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 225 total aligned reads displayed.
GCGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCA > REL606/931240‑931389 | cGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTcc < 2:1176590/76‑1 (MQ=255) gACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCCCTCCa > 2:5156371/1‑76 (MQ=255) gACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCa < 1:7193866/76‑1 (MQ=255) gACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCCCGCTccc > 1:1049261/1‑75 (MQ=255) accccAGCGCCATCGCAATACGCGACGGGAGTTGTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAg < 2:1894098/73‑1 (MQ=255) aCTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCGGCGCTcccg > 2:7320805/1‑74 (MQ=255) tCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCGCTCCAGcc > 1:2539764/1‑76 (MQ=255) cAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCCGCcac > 1:238815/1‑76 (MQ=255) cAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCcac < 1:2979132/76‑1 (MQ=255) aGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCCCTCCCTCcaca > 2:1527590/1‑76 (MQ=255) gcgcCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACAt < 2:4950445/76‑1 (MQ=255) ccccATCGCAATGGGCGACGGGAGTTTTTCTTGCTGAGGGTGGTTGCCCCACGGCAGGCCTCCCCCCAGCCACATc < 2:696363/74‑1 (MQ=255) gcCATCGCAATCCGCGACGGGATTTTTTTTTGCTGATGGTGGTTGCGCCACGGCAGGCCCCGCCCCAGCCACATCa < 2:6144443/76‑1 (MQ=255) gcCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCCCGCTCCCGCCACATCa > 1:6632532/1‑76 (MQ=255) gcCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCa < 1:3997938/76‑1 (MQ=255) gcCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCCCGCTCCCCCCCCATCa > 1:593504/1‑76 (MQ=255) ccATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCGCTCCACCCACCTCAg > 1:6687558/1‑76 (MQ=255) cAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCCCGCCCCAGCCACCTCAAggcggc > 2:5975571/1‑76 (MQ=255) aaTGCGCGACGGTAGTTTTTCTGGCTGGTGGTGTGTGCTCCACGGCGGCCCCCGCCCCAGCCACATCAGGGCGGCa < 2:6836658/76‑1 (MQ=255) aaTACGCGACGGGATTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCa < 2:846596/76‑1 (MQ=255) aaTACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCGCTCCAGCCACATCAGGGGGGCa > 2:2711735/1‑76 (MQ=255) aaTACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCCCGCTCCCGCCACATCCGggcgggc > 1:3097994/1‑74 (MQ=255) tccgcgCCGGGATTTTTTCTTGCGGATGGTGTTGGCCCCAGGGCGGGCCTCGCCCCAGCCACATCAGGGCGGCAat < 2:1284172/74‑1 (MQ=255) tcgcgGCGGGAGTTTTTCTTGCTGATGGTGGTTCCGCCACGGCAGGCCTCCCCCCAGCCACATCAGGGCGGCAata < 1:6011139/75‑1 (MQ=255) aCGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCCAATCACGGCCGCactt > 2:3331951/1‑73 (MQ=255) aCGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCCCGCTCCCGCCACATCAGGGCGGGAata > 1:6494298/1‑76 (MQ=255) gcgACGGGATTTTTTCTTGCGGATGGTGGTTGCCCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATAtt < 1:7125911/76‑1 (MQ=255) gcgACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCCCGGCAGGCCCCGCCCCAGCCACATCAGGGCGGCAATAtt < 2:3122348/76‑1 (MQ=255) gcgACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAATAtt < 1:1833159/76‑1 (MQ=255) gcgACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGCCCTCGCCCCAGCCACATCAGGGCGGCAATAtt < 1:5811711/76‑1 (MQ=255) cgAGGGGATTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTa < 2:2924356/76‑1 (MQ=255) cgACGGTATTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTa < 2:7397068/76‑1 (MQ=255) cgACGGTAGTTTTTCTTGCTGATGGTGGTGGCGCCACGGCAGGCCTCCCCCCAGCCACATCAGGGCGGCAATATTa < 2:6524159/76‑1 (MQ=255) cgACGGGAGTTTTTCTTGCTGATGGGGGTTCCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTa < 2:3785262/76‑1 (MQ=255) ggcGGGAGTTTTTCTTGCTGATGGTGGTTCCCCCGCGGCGCGCCTCGCTCCAGCCACATCAGGGCGGCAATATTac < 2:4811701/74‑1 (MQ=255) cGGGATTTTTTCTGGCTGATGTGGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTacac < 1:4345009/76‑1 (MQ=255) cGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGCCCTCGCTCCAGCCACATCAGGGCGGCAATATTacac < 2:1610607/76‑1 (MQ=255) gggTGTTTTTCTTGCTGATGGTGGTTGCCCCACGGCAGGCCGCCCTCCAGCCACATCAGGGCGGCAATATTAcaca < 2:5349536/76‑1 (MQ=255) ggATTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAg < 2:4622820/76‑1 (MQ=255) gAGTTTTTCTTGCTGATGGTGGTTGCCCCCCGGCAGCCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGt < 1:6838149/76‑1 (MQ=255) attttttCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTa < 1:6889053/74‑1 (MQ=255) aGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCCCGCTCCAGCCCCATCAGGGCGGGAATATTACACAGTa > 1:7327280/1‑76 (MQ=255) tttttCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTaaa < 1:1093331/76‑1 (MQ=255) tttCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCCGCCACATCCAGGCGGCGATATTACACAGTaaaaa > 1:5529488/1‑75 (MQ=255) ttCTTGCTGATGGTGGTGGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATa < 2:4679105/76‑1 (MQ=255) ttCTTGCGGAGGGTGGTTGCGCCACGGCGGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATa < 2:7615238/76‑1 (MQ=255) tCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATaa < 1:7440564/76‑1 (MQ=255) tCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATaa < 2:5684241/76‑1 (MQ=255) tCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCCGCCACAACCGGGCGGCAATACTACACCGTAaaaaaa > 2:5515417/1‑76 (MQ=255) tttGCTGATGGTGGTTGCGCCGCGGCGGGCCTCCCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAAc < 2:1040066/75‑1 (MQ=255) tGCTGTTGGTGGTGGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACgg < 2:6062392/76‑1 (MQ=255) cTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTa > 1:7617173/1‑76 (MQ=255) cTGATGGTGGTTCCCCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTa < 2:5142885/76‑1 (MQ=255) cTGATGGTGGTGGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTa < 2:339503/76‑1 (MQ=255) tgttggtggTTGCCCCCCGGCAGGCCGCTCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTaa < 1:5560364/73‑1 (MQ=255) tGATGGTGGTTGCGCCACGGCAGGGCTCTCTCCCGCCCCATCCAGGCGGCAATATTAAACACTAAAATAAACGTaa > 2:2301665/1‑76 (MQ=255) tGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTaa < 2:5207182/76‑1 (MQ=255) tGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTaa < 2:3011949/76‑1 (MQ=255) tGATGGGGGCTCCGCCACGGCAGCCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTaa < 2:6363281/76‑1 (MQ=255) gagggtggtGGCCCCACGGCAGCCCTCCCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAAt < 1:137124/73‑1 (MQ=255) gtggtggTTGCCCCACGGCGGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATa < 1:3382060/75‑1 (MQ=255) aTGGTGGTTGCGCCCCGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATa < 2:837465/76‑1 (MQ=255) aTGGTGGTTGCGCCACGGCAGGGCTCGCTCCCGCCACATCAGGGCGGCCATATTACACAGTAAAATAAAGGGAATa > 2:1972367/1‑76 (MQ=255) aTGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCCATATTACACAGTAAAAAAAAGGGAATa > 2:4158932/1‑76 (MQ=255) ggtggtTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACt < 1:185930/76‑1 (MQ=255) gggggtTGCGCCACCGCAGGCCCCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACt < 1:6034174/73‑1 (MQ=255) tgtgtTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTg < 2:1783498/73‑1 (MQ=255) gtggtTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTg < 1:804049/76‑1 (MQ=255) gtggtTGCGCCACGGCAGGCCCCGCTCCAACCACATCCAGGCGGCACTATTTAACAGCAAAATAACGGGAAAACTg > 2:6727587/1‑76 (MQ=255) tggtggCGCCACGGCAGGCCTCCCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGa < 1:4870860/76‑1 (MQ=255) tggtTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGa > 2:4466172/1‑76 (MQ=255) gggtTGCGCCGCGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGa < 1:5425294/75‑1 (MQ=255) tGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGc > 1:2921135/1‑76 (MQ=255) tGCCCCACGGCAGCCCTCCCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGc < 1:5279889/76‑1 (MQ=255) cgccgcGGCGGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAg < 2:4151001/76‑1 (MQ=255) cgcCACGGCAGTCCTGGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAg < 2:4023470/76‑1 (MQ=255) cgcCACGGCAGGGCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATAATGACGGCAg > 2:2580430/1‑76 (MQ=255) gcCCCGGGAGCCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGa < 1:6066674/76‑1 (MQ=255) gcCAGGGCAGACCCCCCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGa < 1:5792203/76‑1 (MQ=255) gcCACGGCAGGGCTCCCTCCAGCCACACCCGGGCGGCAATATTACACACTAAAATAACGGGAATACTTATGGCAGa > 2:4242638/1‑76 (MQ=255) cccGGCCGACCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGaat < 1:4490346/74‑1 (MQ=255) caaggCAGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGaat < 1:1942653/73‑1 (MQ=255) cgggCAGCCCCCCCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGaata < 2:2128792/74‑1 (MQ=255) cGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGaaaaa > 2:5485894/1‑76 (MQ=255) cGGCAGCCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGaataa < 1:3471483/76‑1 (MQ=255) gaGGCCCCCCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGc < 1:3686416/75‑1 (MQ=255) cAGGCCTCACTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGc < 2:5757566/76‑1 (MQ=255) cAGCCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGc < 1:2606918/76‑1 (MQ=255) aGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCa > 1:4134842/1‑76 (MQ=255) aGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCa > 1:3878709/1‑76 (MQ=255) aGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCa > 2:7430084/1‑76 (MQ=255) aGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCa > 1:5149217/1‑76 (MQ=255) aGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAAAATAATgaa > 2:1145100/1‑74 (MQ=255) aGGCCTCGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCa < 1:2142760/76‑1 (MQ=255) ccTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGaa > 1:851668/1‑76 (MQ=255) cTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAAt > 2:1363393/1‑76 (MQ=255) ccGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATc < 1:4778764/75‑1 (MQ=255) cGCCCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAAtag < 2:6546805/76‑3 (MQ=255) cTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGcc > 2:3811509/1‑76 (MQ=255) tCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCa > 1:1581803/1‑76 (MQ=255) | GCGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCA > REL606/931240‑931389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |