| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,928,595 | G→A | A127T (GCC→ACC) | rho → | transcription termination factor Rho |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,928,595 | 0 | G | A | 98.9% | 1041.2 / NA | 277 | A127T (GCC→ACC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (137/136); minor base C (0/3); total (137/140) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 279 total aligned reads displayed.
CCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTA > REL606/3928522‑3928667 | cccgccGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACAcc > 1:4313616/1‑76 (MQ=255) cgccgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCg < 1:4581128/76‑1 (MQ=255) gccgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGc < 2:4308352/76‑1 (MQ=255) aaaGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGcaaca > 2:4623266/1‑76 (MQ=255) aagaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGcaacaa > 2:5444508/1‑76 (MQ=255) aagaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGcaacaa < 1:4897166/76‑1 (MQ=255) aagaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGcaacaa < 1:4069232/76‑1 (MQ=255) agaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACaaa > 1:4198169/1‑76 (MQ=255) agaagGAGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACaaa > 2:5182765/1‑76 (MQ=255) gaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACaaaa > 2:5305162/1‑76 (MQ=255) gaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACaaaa < 1:3039217/76‑1 (MQ=255) aagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAAt < 1:1510587/76‑1 (MQ=255) gTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCct < 2:2910637/76‑1 (MQ=255) gTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCct < 2:3893499/76‑1 (MQ=255) gAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCctct < 2:79198/76‑1 (MQ=255) gAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCctct > 2:6579642/1‑76 (MQ=255) gAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCctct > 1:747138/1‑76 (MQ=255) cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTg < 1:1185060/76‑1 (MQ=255) cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTg < 2:1538853/76‑1 (MQ=255) cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTg < 2:1261368/76‑1 (MQ=255) cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTg < 2:892259/76‑1 (MQ=255) gCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTga < 1:982801/76‑1 (MQ=255) cTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTgag < 1:5617501/76‑1 (MQ=255) cTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTgag < 1:1577293/76‑1 (MQ=255) tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGAGAAACCTGAAAACACCCGCAACAAAATCCTCTTTgaga < 1:1053599/76‑1 (MQ=255) tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTgaga > 2:3342160/1‑76 (MQ=255) tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTgaga > 1:5681478/1‑76 (MQ=255) tATTTTGCGCTGCTGAAAGTTAACGAAGTAAACTTCGACAAACCAGAAAACACCCGCAACAAAATCCTCTTTgaga < 2:3172193/76‑1 (MQ=255) aTTTTNCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGaa > 2:2840412/1‑76 (MQ=255) aTTTTGCGCTGCTGAATGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGaa < 1:3551903/76‑1 (MQ=255) aTTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGaa < 1:6888798/76‑1 (MQ=255) ttGCGCTGCTGAAAGTTAACGAAGTTAGCTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACtt < 2:931068/76‑1 (MQ=255) ttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACtt < 1:5837850/76‑1 (MQ=255) ttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACtt > 2:661349/1‑76 (MQ=255) ttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACtt > 1:3936767/1‑76 (MQ=255) tGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACCCCCGCAACAAAATCCTCTTTGAGAACTTa < 2:2487634/76‑1 (MQ=255) tGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTa > 2:7117376/1‑76 (MQ=255) gcgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTaa < 1:596738/76‑1 (MQ=255) cgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAAc < 1:173415/76‑1 (MQ=255) cgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAAc < 1:6532902/76‑1 (MQ=255) cgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAAc < 2:395034/76‑1 (MQ=255) cgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAAc < 2:1847602/76‑1 (MQ=255) gctgctGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAAcc < 2:4871640/76‑1 (MQ=255) gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg > 2:735735/1‑76 (MQ=255) gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg < 2:1773407/76‑1 (MQ=255) ctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGc > 2:1607599/1‑76 (MQ=255) gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:3245516/1‑76 (MQ=255) aaGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCa > 2:5014876/1‑76 (MQ=255) aaGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCa < 2:3448573/76‑1 (MQ=255) aaGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCa > 1:2049814/1‑76 (MQ=255) aGTTAACGTAGTCAACTTCGACAAACCTGAAAGCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc < 2:4501309/76‑1 (MQ=255) aGTTAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc < 1:5094990/76‑1 (MQ=255) tAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa > 1:800513/1‑76 (MQ=255) tAACGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa > 2:1934102/1‑76 (MQ=255) aaCGAATTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa < 1:4838071/76‑1 (MQ=255) aaCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa > 1:2558934/1‑76 (MQ=255) aaCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa > 2:5790914/1‑76 (MQ=255) aaCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa > 1:3744725/1‑76 (MQ=255) aaCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa < 1:5208427/76‑1 (MQ=255) aCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaaa < 2:5413181/76‑1 (MQ=255) aCGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaaa < 2:1636281/76‑1 (MQ=255) cGAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc < 1:4438408/76‑1 (MQ=255) gAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAct > 1:2827858/1‑76 (MQ=255) gAAGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAct > 2:4011710/1‑76 (MQ=255) aGTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActct > 1:7042649/1‑76 (MQ=255) gTTAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc < 2:7042749/76‑1 (MQ=255) ttAACTTCTACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg > 1:1867167/1‑76 (MQ=255) ttAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg > 1:6443982/1‑76 (MQ=255) ttAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg > 2:418220/1‑76 (MQ=255) ttAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg > 2:5601625/1‑76 (MQ=255) ttAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg > 1:3405925/1‑76 (MQ=255) tAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGt < 2:5695774/76‑1 (MQ=255) tAACTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGt > 2:6317006/1‑76 (MQ=255) aCTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt < 1:6029308/76‑1 (MQ=255) aCTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt < 1:2167525/76‑1 (MQ=255) aCTTCCACAAACCTGAAACCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt < 1:6322194/76‑1 (MQ=255) cTTCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTg < 1:3180226/76‑1 (MQ=255) ttCGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc > 1:5428027/1‑76 (MQ=255) cGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGt > 1:720721/1‑76 (MQ=255) cGACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGt < 2:7467669/76‑1 (MQ=255) gACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTa < 2:2752768/76‑1 (MQ=255) gACAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTa > 2:2775845/1‑76 (MQ=255) aCAAACCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTAt > 1:4996996/1‑76 (MQ=255) cAAACCTGAAAACACCCGCAACAAAATCTTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATg > 2:1368624/1‑76 (MQ=255) aaaCCTGAAAACCCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATgg < 1:927177/76‑1 (MQ=255) aaaCCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATgg > 2:3736734/1‑76 (MQ=255) aaaCCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATgg < 2:2370122/76‑1 (MQ=255) aaCCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa < 2:6700511/76‑1 (MQ=255) aaCCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa < 2:2206721/76‑1 (MQ=255) aCCTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGaa > 1:4379071/1‑76 (MQ=255) ccTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAAc > 1:266196/1‑76 (MQ=255) cTGAAAACCCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg < 1:626247/76‑1 (MQ=255) cTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg < 2:6184487/76‑1 (MQ=255) cTGAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg > 2:2684706/1‑76 (MQ=255) taaaaaCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGt < 1:5858939/74‑1 (MQ=255) gAAAACACCCGCAACAAAATCCTCTTTGAGAACTTAATCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg < 2:567408/76‑1 (MQ=255) aaaCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt > 1:1418055/1‑76 (MQ=255) aaaCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt < 2:3851866/76‑1 (MQ=255) aaaCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt < 1:668775/76‑1 (MQ=255) aaCACCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTa > 1:4712480/1‑76 (MQ=255) | CCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTA > REL606/3928522‑3928667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |