| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,162,570 | T→A | I572N (ATC→AAC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,162,570 | 0 | T | A | 99.2% | 956.2 / ‑0.6 | 236 | I572N (ATC→AAC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base A (123/110); total (125/111) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01 | |||||||||||
Only 100 of 239 total aligned reads displayed.
CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGA > REL606/4162497‑4162642 | cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACa > 1:2219465/1‑76 (MQ=255) gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACaa < 1:55643/76‑1 (MQ=255) gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACaa < 1:7346874/76‑1 (MQ=255) aCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑ctc > 1:3001584/1‑76 (MQ=255) aCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑ctc < 1:7568937/76‑1 (MQ=255) cGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑ctct < 2:4772372/76‑1 (MQ=255) gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑ctctc > 2:5202615/1‑76 (MQ=255) tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑ctctct < 2:2910145/76‑1 (MQ=255) cacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGt > 2:1194196/1‑76 (MQ=255) cccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTcc > 1:1330218/1‑76 (MQ=255) cccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTcc > 1:4845265/1‑76 (MQ=255) cccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTcc > 1:3616058/1‑76 (MQ=255) gACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCgtg > 2:2172426/1‑76 (MQ=255) cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTa < 2:3094323/76‑1 (MQ=255) cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTa < 2:4369090/76‑1 (MQ=255) cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTa > 1:5793868/1‑76 (MQ=255) tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTCTGTCCgtgt > 2:3185122/1‑76 (MQ=255) tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTAc < 1:6394330/76‑1 (MQ=255) tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGCCCGTGTAc > 1:1019152/1‑76 (MQ=255) cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACg > 1:4515883/1‑76 (MQ=255) aCTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGc < 2:1917090/76‑1 (MQ=255) aCTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGc > 1:7070470/1‑76 (MQ=255) cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGca > 1:4663259/1‑76 (MQ=255) cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGca > 2:7438266/1‑76 (MQ=255) tACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGcac > 1:7538064/1‑76 (MQ=255) tACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGcac < 2:2857415/76‑1 (MQ=255) aCGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGcaca > 1:6942202/1‑76 (MQ=255) cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAg < 2:4632328/76‑1 (MQ=255) cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAg > 2:1393406/1‑76 (MQ=255) cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAg < 2:3202729/76‑1 (MQ=255) ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGa < 2:1122687/76‑1 (MQ=255) gTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGAc < 1:7203063/76‑1 (MQ=255) tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACt < 2:3304923/76‑1 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTa > 1:2865036/1‑76 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTa > 1:825480/1‑76 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTa > 1:4569011/1‑76 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTa > 1:4260520/1‑76 (MQ=255) gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTaa > 2:548130/1‑76 (MQ=255) gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTaa < 1:7382334/76‑1 (MQ=255) cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAAc < 1:5133472/76‑1 (MQ=255) cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAAc > 1:3042383/1‑76 (MQ=255) cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAAc > 2:3783594/1‑76 (MQ=255) cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAAc > 2:593898/1‑76 (MQ=255) gTATGTCCAATCGAAACCCCTGAAGTTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACg < 1:2197316/76‑1 (MQ=255) gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACg < 2:2667893/76‑1 (MQ=255) tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGa > 2:1005246/1‑76 (MQ=255) aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGaa < 1:976170/76‑1 (MQ=255) aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGaa < 1:6047997/76‑1 (MQ=255) aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGaa < 2:1520806/76‑1 (MQ=255) aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGaa > 2:553101/1‑76 (MQ=255) tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAAt > 1:2722461/1‑76 (MQ=255) tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAAt < 2:4362819/76‑1 (MQ=255) tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAAt > 2:7619502/1‑76 (MQ=255) gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATa < 1:6658768/76‑1 (MQ=255) gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATa < 1:6500327/76‑1 (MQ=255) tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATAc < 1:892811/76‑1 (MQ=255) tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATAc < 2:61119/76‑1 (MQ=255) tCCAATCGAAACCCCTAAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATAc > 2:6961444/1‑76 (MQ=255) ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACg > 2:6317668/1‑76 (MQ=255) ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACg < 2:245163/76‑1 (MQ=255) ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACg > 2:66374/1‑76 (MQ=255) cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACgg > 1:414005/1‑76 (MQ=255) cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACgg > 2:6082816/1‑76 (MQ=255) tCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt < 1:7246585/76‑1 (MQ=255) aaaCCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCt > 2:3452062/1‑76 (MQ=255) aCCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCttt > 2:5305012/1‑75 (MQ=255) ccccTGAAGGTCCGAACGTCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTga < 2:3513635/76‑1 (MQ=255) cccTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag > 1:3645970/1‑76 (MQ=255) ccTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgaga < 2:6025755/76‑1 (MQ=255) cTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAc < 1:538866/76‑1 (MQ=255) tGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt < 2:5283278/76‑1 (MQ=255) aaGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc < 1:1208501/76‑1 (MQ=255) aaGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc < 2:7278853/76‑1 (MQ=255) aaGGGCCGAACATCGGTCTGACCAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc < 1:145376/76‑1 (MQ=255) aGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTTAGACTccc > 2:2804503/1‑75 (MQ=255) ggTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAcccccc > 1:3197566/1‑74 (MQ=255) ggTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt < 2:1211700/76‑1 (MQ=255) ggTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt < 2:4015770/76‑1 (MQ=255) gTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTa < 1:3299632/76‑1 (MQ=255) tCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTAt < 2:85531/76‑1 (MQ=255) tCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTAt < 1:3698896/76‑1 (MQ=255) ccGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATc < 2:7086101/76‑1 (MQ=255) cGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTGAGACTCCCCATCg > 2:62753/1‑76 (MQ=255) aaCATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTTCGACTCCCTATCGTa > 1:4454811/1‑76 (MQ=255) aaCATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTTAGACTCCGTATCATa > 2:1749955/1‑76 (MQ=255) aaCATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa < 1:255655/76‑1 (MQ=255) aCATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTTAGAATCCCGCTCGTaa > 2:2498950/1‑76 (MQ=255) aCATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaa < 2:5961465/76‑1 (MQ=255) cATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa < 1:199577/76‑1 (MQ=255) tCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGt < 1:3917587/76‑1 (MQ=255) cGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTGAGACTCCCTATCGTAAAGTg > 2:3567686/1‑76 (MQ=255) cGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg < 1:2027141/76‑1 (MQ=255) cGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg > 1:7466394/1‑76 (MQ=255) cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTGAGACTCCGTATCCTAAAATGAccc > 1:2353422/1‑75 (MQ=255) cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg > 1:1744199/1‑76 (MQ=255) cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg < 2:4948690/76‑1 (MQ=255) cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg < 1:1129735/76‑1 (MQ=255) cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg < 1:6241669/76‑1 (MQ=255) cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAACg > 2:3855230/1‑76 (MQ=255) tGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGa > 2:5730128/1‑76 (MQ=255) | CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGA > REL606/4162497‑4162642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |