| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,698,011 | G→A | S3L (TCG→TTG) | slyA ← | transcriptional regulator SlyA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,698,011 | 0 | G | A | 98.8% | 1045.1 / 1.1 | 247 | S3L (TCG→TTG) | slyA | transcriptional regulator SlyA |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (89/155); total (91/156) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
Only 100 of 248 total aligned reads displayed.
CAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTG > REL606/1697938‑1698083 | cAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAAt < 2:1993347/76‑1 (MQ=255) aGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAAtt > 2:4425680/1‑76 (MQ=255) aGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAAtt < 1:4248067/76‑1 (MQ=255) gCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTc < 1:6052980/76‑1 (MQ=255) gCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTc < 2:431459/76‑1 (MQ=255) ggTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCa < 1:2227496/76‑1 (MQ=255) ggTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCa < 1:2880179/76‑1 (MQ=255) ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAAtt > 1:1640909/1‑76 (MQ=255) ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAAtt < 1:2163321/76‑1 (MQ=255) ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAAtt > 1:4699724/1‑76 (MQ=255) tCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAAttt < 2:333923/76‑1 (MQ=255) tCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAAttt > 1:2074078/1‑76 (MQ=255) ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAt < 1:5262896/76‑1 (MQ=255) ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAt < 2:3651974/76‑1 (MQ=255) gCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAtc < 1:6092357/76‑1 (MQ=255) gCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAtc < 2:4243731/76‑1 (MQ=255) cGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAtct > 2:3705573/1‑76 (MQ=255) cGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAtct > 2:68675/1‑76 (MQ=255) aTGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTcc > 2:3838898/1‑76 (MQ=255) ggTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCtt > 1:5665091/1‑76 (MQ=255) gTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTta < 2:7598444/76‑1 (MQ=255) tCTATCAGAGCACGCCATATGCGCACCAACCGTTCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtat < 1:3175333/76‑1 (MQ=255) tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtat < 2:1033044/76‑1 (MQ=255) tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtat > 2:2373228/1‑76 (MQ=255) tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtat < 1:5115841/76‑1 (MQ=255) cTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtata < 1:2953261/76‑1 (MQ=255) cTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtata > 1:1405440/1‑76 (MQ=255) tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATaa < 2:1483133/76‑1 (MQ=255) tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATaa < 2:7519301/76‑1 (MQ=255) tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATaa > 2:1942328/1‑76 (MQ=255) tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATaa < 1:4580744/76‑1 (MQ=255) tCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAAtt < 2:5210412/76‑1 (MQ=255) tCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAAtt > 2:6331340/1‑76 (MQ=255) tCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAAtt < 1:120226/76‑1 (MQ=255) cAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTa > 1:7520480/1‑76 (MQ=255) cAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTa > 2:2585132/1‑76 (MQ=255) agagCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAg < 1:3940932/76‑1 (MQ=255) agCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCt < 1:645446/76‑1 (MQ=255) gCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTa < 2:1372756/76‑1 (MQ=255) gCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTa < 1:3465691/76‑1 (MQ=255) gCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTa < 1:7362585/76‑1 (MQ=255) ccATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTaa < 1:3092919/76‑1 (MQ=255) cATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAg > 2:2826064/1‑76 (MQ=255) tatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGat < 1:7092727/76‑1 (MQ=255) tatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGat < 2:2245299/76‑1 (MQ=255) tatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGat < 1:3788368/76‑1 (MQ=255) atGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata > 1:1261293/1‑76 (MQ=255) atGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata < 1:4647202/76‑1 (MQ=255) tGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGatat < 2:487312/76‑1 (MQ=255) gcgcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatt < 2:821217/76‑1 (MQ=255) gcgcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatt < 2:5132201/76‑1 (MQ=255) gcgcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatt < 2:5396148/76‑1 (MQ=255) cgcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatta > 2:6757233/1‑76 (MQ=255) cgcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatta < 1:80889/76‑1 (MQ=255) gcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtattat < 1:1687283/76‑1 (MQ=255) gcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtattat < 2:6274985/76‑1 (MQ=255) cACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg > 2:3191647/1‑76 (MQ=255) ccAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg > 1:6983056/1‑76 (MQ=255) cAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCgg > 2:4151580/1‑76 (MQ=255) aaCCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGc > 2:6482737/1‑76 (MQ=255) ccGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtt < 1:4877554/76‑1 (MQ=255) cGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCttt < 1:6583924/76‑1 (MQ=255) gTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtttt < 2:911017/76‑1 (MQ=255) tGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTa < 2:5529711/76‑1 (MQ=255) tGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTa < 2:2810074/76‑1 (MQ=255) gCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg < 1:2479727/76‑1 (MQ=255) gCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg < 1:7382353/76‑1 (MQ=255) cAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGaa < 2:1846694/76‑1 (MQ=255) cAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGaa < 2:608152/76‑1 (MQ=255) aGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAt < 1:2101164/76‑1 (MQ=255) gATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATa < 1:5018581/76‑1 (MQ=255) gATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATa < 2:4697207/76‑1 (MQ=255) aTCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAg < 1:432378/76‑1 (MQ=255) aTCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAg < 2:3979230/76‑1 (MQ=255) tCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgt < 1:4108860/76‑1 (MQ=255) tCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgt > 2:2188508/1‑76 (MQ=255) cAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtg > 2:2148755/1‑76 (MQ=255) aGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtgt < 1:350007/76‑1 (MQ=255) aGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtgt > 2:7085306/1‑76 (MQ=255) gAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGtgtg < 2:4567321/76‑1 (MQ=255) gAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGtgtg < 2:5759020/76‑1 (MQ=255) gAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGtgtg > 2:2736889/1‑76 (MQ=255) aaCCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGtgtgg > 2:6031117/1‑75 (MQ=255) aaCCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgc > 1:4162649/1‑76 (MQ=255) aCCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgca < 1:2608621/76‑1 (MQ=255) ccTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcag < 2:4472914/76‑1 (MQ=255) ccTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcag > 1:327278/1‑76 (MQ=255) ccTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcag > 2:1738487/1‑76 (MQ=255) cTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc > 1:2807106/1‑76 (MQ=255) cTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc > 2:5696557/1‑76 (MQ=255) cTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc < 1:549368/76‑1 (MQ=255) cTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc > 2:1153555/1‑76 (MQ=255) tAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagca < 1:1174256/76‑1 (MQ=255) tAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagca < 1:419300/76‑1 (MQ=255) tgTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCaa > 2:6599894/2‑76 (MQ=255) aGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCaa > 2:4858620/1‑76 (MQ=255) aGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCaa < 2:7170261/76‑1 (MQ=255) gTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAAt < 2:125031/76‑1 (MQ=255) tGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAAtt < 1:1101083/76‑1 (MQ=255) ggCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTg > 1:4900638/1‑76 (MQ=255) | CAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTG > REL606/1697938‑1698083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |