BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	636.6 / NA	164	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (59/105); total (59/105)

Only 100 of 188 total aligned reads displayed.

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATcc                                                                        <  1:7437438/76‑1 (MQ=255)

gCCGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAG‑CCCCCAAAAAAGCAAAaaaccaaacc                                                                        >  1:5101038/1‑76 (MQ=255)

  cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAG‑CCCCCATAACAGCAAAAAGCCAAAcccc                                                                      >  1:5299800/1‑76 (MQ=255)

    aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCccac                                                                    <  1:3830526/76‑1 (MQ=255)

      gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCccacca                                                                  <  1:14858/76‑1 (MQ=255)

      gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCccacca                                                                  <  1:3150316/76‑1 (MQ=255)

      gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAATCAA‑CCCCCAAAACAGCAAAACGCCAAACCccccca                                                                  >  1:1121772/1‑76 (MQ=255)

       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                 <  2:4711606/76‑1 (MQ=255)

       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                 <  1:1067148/76‑1 (MQ=255)

       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                 <  2:3491262/76‑1 (MQ=255)

       cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAG‑CCCCAAAAACAGCAAAACACCCAACCccacccc                                                                 >  2:4471844/1‑74 (MQ=255)

        aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTTGGGAAAAAG‑CCCCAACAAAAGCCAAACGCCCAACcccccccaa                                                                >  2:4433795/1‑73 (MQ=255)

        aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGGAATCAG‑CCCCAATAACAGCAAAACGCCAAACCCCACCAGa                                                                >  1:1007794/1‑76 (MQ=255)

        aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGCCCCCAAAAACA‑CAAAACGCCCAACCccaccacc                                                                >  2:352598/1‑74 (MQ=255)

         tCCGGCGTCCAGGCTCGGCTGGTCTGGCTGGCGGAAACCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                               <  2:3818076/76‑1 (MQ=255)

         tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                               <  1:6688861/76‑1 (MQ=255)

         tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                               <  2:4471668/76‑1 (MQ=255)

          ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                              <  1:7257756/76‑1 (MQ=255)

          ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                              <  1:1017799/76‑1 (MQ=255)

          ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                              <  1:1415773/76‑1 (MQ=255)

            ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                            <  2:4444110/76‑1 (MQ=255)

             gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                                                           <  2:4495066/76‑1 (MQ=255)

              cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                                                          <  2:2321412/76‑1 (MQ=255)

                 ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                       <  2:3325303/76‑1 (MQ=255)

                 ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                       <  2:6500385/76‑1 (MQ=255)

                   aGGCGGGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                     <  1:2609049/76‑1 (MQ=255)

                   aGGCGCGGCTGGTCTGGGTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                     <  2:4992606/76‑1 (MQ=255)

                   aGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                     <  1:1896145/76‑1 (MQ=255)

                      ctcgGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGcc                                                  <  1:1332262/74‑1 (MQ=255)

                      cgcgGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGcc                                                  <  2:6896296/76‑1 (MQ=255)

                      cgcgGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAAAGCCAATCCCCACCCGATAAAGCATGACGcc                                                  >  2:6560187/1‑76 (MQ=255)

                      cgcgGCTGGGCTGGCTGTCGGAAAACAG‑CCCCCAAAACAGCAAAACGCCAAACCCCACCAGATAAAACAAGACAcc                                                  >  2:5897173/1‑76 (MQ=255)

                        cgGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:564932/76‑1 (MQ=255)

                        cgGCTGGTCTGGCTGTCGGAAAACAG‑CCCCCATAACAGCAAAAAGCCCATCCCCACCCGATAAAGCATGACGcccg                                                >  2:4103155/1‑74 (MQ=255)

                         ggCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               <  2:3361381/76‑1 (MQ=255)

                          gCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              >  1:411766/1‑76 (MQ=255)

                           cTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  1:7618681/76‑1 (MQ=255)

                           cTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  2:2232076/76‑1 (MQ=255)

                            tGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  2:2459192/1‑76 (MQ=255)

                            tGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  2:1110512/76‑1 (MQ=255)

                             ggTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  1:4380141/1‑76 (MQ=255)

                             ggTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  2:2588154/1‑76 (MQ=255)

                              gTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:5502040/76‑1 (MQ=255)

                               tCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  2:1009338/76‑1 (MQ=255)

                                cTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGGATGACGCCTGGATTAAAc                                        <  2:5356017/76‑1 (MQ=255)

                                cTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:3124580/1‑76 (MQ=255)

                                 tGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       <  2:7443915/76‑1 (MQ=255)

                                 tGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       <  1:6295822/76‑1 (MQ=255)

                                 tGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       <  1:492469/76‑1 (MQ=255)

                                   gCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                     >  1:5801450/1‑76 (MQ=255)

                                    cTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  2:7083110/1‑76 (MQ=255)

                                      gTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:5966541/76‑1 (MQ=255)

                                      gTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  2:7320394/1‑76 (MQ=255)

                                       tCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 >  1:7321769/1‑76 (MQ=255)

                                       tCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:2461889/76‑1 (MQ=255)

                                       tCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  1:1389143/76‑1 (MQ=255)

                                        cGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  2:2620448/1‑76 (MQ=255)

                                         ggAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  1:7026386/76‑1 (MQ=255)

                                         ggAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               >  1:291183/1‑76 (MQ=255)

                                          gAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  1:1469283/76‑1 (MQ=255)

                                          gAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:7559203/76‑1 (MQ=255)

                                            aaTCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            >  1:5909395/1‑76 (MQ=255)

                                             aTCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  2:356583/76‑1 (MQ=255)

                                              tCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          >  2:1498499/1‑76 (MQ=255)

                                               cAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:7161593/76‑1 (MQ=255)

                                                aG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  2:1333895/76‑1 (MQ=255)

                                                aG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  2:1801353/76‑1 (MQ=255)

                                                 g‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       <  1:4432477/76‑1 (MQ=255)

                                                 g‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       <  1:387800/76‑1 (MQ=255)

                                                 g‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       >  2:2066841/1‑76 (MQ=255)

                                                   ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      >  2:3902972/1‑76 (MQ=255)

                                                   ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      <  2:2502019/76‑1 (MQ=255)

                                                    cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                     >  1:1506148/1‑76 (MQ=255)

                                                    cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                     >  1:6146861/1‑76 (MQ=255)

                                                      cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                   <  1:2040989/76‑1 (MQ=255)

                                                      cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                   <  2:6426611/76‑1 (MQ=255)

                                                       aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  1:885288/1‑76 (MQ=255)

                                                       aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  1:4563392/1‑76 (MQ=255)

                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 <  1:1692199/76‑1 (MQ=255)

                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  1:2626388/1‑76 (MQ=255)

                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 <  2:5043270/76‑1 (MQ=255)

                                                          aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  2:1148530/1‑76 (MQ=255)

                                                           aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg              >  1:3354653/1‑76 (MQ=255)

                                                           aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg              <  2:963198/76‑1 (MQ=255)

                                                           aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg              <  1:1289836/76‑1 (MQ=255)

                                                            cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             >  2:6933920/1‑76 (MQ=255)

                                                             aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa            <  2:6413484/76‑1 (MQ=255)

                                                             aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa            >  1:5905483/1‑76 (MQ=255)

                                                             aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa            <  2:1501456/76‑1 (MQ=255)

                                                              gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  2:553758/76‑1 (MQ=255)

                                                              gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           >  2:1903831/1‑76 (MQ=255)

                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        >  2:5208785/1‑76 (MQ=255)

                                                                  aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       <  2:7508021/76‑1 (MQ=255)

                                                                   aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      >  1:6437543/1‑76 (MQ=255)

                                                                   aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      >  1:1635717/1‑76 (MQ=255)

                                                                     gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc    >  1:1740905/1‑76 (MQ=255)

                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  1:5978855/1‑76 (MQ=255)

                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  2:2558486/1‑76 (MQ=255)

                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:3007656/1‑76 (MQ=255)

                                                                       cAACCCCCCCCAGATAAAGCATGCCGCCTGTATTAAACAGGTTTTTTCCCGATGGCGCACCGCCCCCCAGCCGCca  <  2:1638262/76‑1 (MQ=255)

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`