| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,251,805 | C→G | G319A (GGC→GCC) | nusA ← | transcription elongation factor NusA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,251,805 | 0 | C | G | 99.0% | 825.5 / ‑3.2 | 202 | G319A (GGC→GCC) | nusA | transcription elongation factor NusA |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base G (79/121); total (79/123) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
Only 100 of 206 total aligned reads displayed.
GTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAG > REL606/3251732‑3251877 | gtcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCa > 2:6123668/1‑76 (MQ=255)gtcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCa < 1:5461463/76‑1 (MQ=255)gtcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCa < 1:2802772/76‑1 (MQ=255)gtcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCa < 1:2385810/76‑1 (MQ=255) tcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCaa > 1:4792932/1‑76 (MQ=255) cgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAAt > 1:7354219/1‑76 (MQ=255) cgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAAt < 1:6475797/76‑1 (MQ=255) cgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAAt > 2:3082045/1‑76 (MQ=255) gtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATc < 2:6731965/76‑1 (MQ=255) tcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCg < 1:7255305/76‑1 (MQ=255) tcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCg < 2:294922/76‑1 (MQ=255) cccaCGGTCATCGCGTTGAGTTCCCACCCGCCCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCg < 1:6838621/73‑1 (MQ=255) aaCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGcc > 1:7308928/1‑76 (MQ=255) aaCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGcc > 1:4496289/1‑76 (MQ=255) aCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCt < 1:1122221/76‑1 (MQ=255) aCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCt < 2:1978344/76‑1 (MQ=255) aCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCt < 2:4269395/76‑1 (MQ=255) aCGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCt > 1:2022991/1‑76 (MQ=255) aCGGTCATCACGTTGAGTGCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCt < 2:4416716/76‑1 (MQ=255) ggTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTgc > 2:3967710/1‑76 (MQ=255) catcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGcc > 2:72033/1‑76 (MQ=255) catcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGcc > 2:1414952/1‑76 (MQ=255) catcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGcc > 1:4318408/1‑76 (MQ=255) catcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGcc > 1:5674604/1‑76 (MQ=255) gtcaCGTTGAGTCCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCa < 2:5302754/75‑1 (MQ=255) atcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCa < 1:5731971/76‑1 (MQ=255) tcaCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAg > 1:4887368/1‑76 (MQ=255) caCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAgg > 2:1194156/1‑76 (MQ=255) caCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAgg > 1:3894083/1‑76 (MQ=255) cGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGtt < 2:5788415/76‑1 (MQ=255) cGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGtt < 1:5540029/76‑1 (MQ=255) gTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTa > 2:2446273/1‑76 (MQ=255) tGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTAcc > 1:6878640/1‑76 (MQ=255) gAGTTCCCACCCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCg < 1:5938321/76‑1 (MQ=255) gAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCg < 1:5079142/76‑1 (MQ=255) aGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCgg > 1:5122585/1‑76 (MQ=255) ttCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCt < 1:35369/76‑1 (MQ=255) ttCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCt > 1:3507839/1‑76 (MQ=255) ttCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCt > 1:1198990/1‑76 (MQ=255) tCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCtt > 2:110999/1‑76 (MQ=255) cccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTc < 2:5244646/76‑1 (MQ=255) cccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTc > 2:5908335/1‑76 (MQ=255) ccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCtttc > 1:674618/1‑74 (MQ=255) cAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCaa < 1:2477723/76‑1 (MQ=255) cAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAAGCGCCTGCGCCAGGTTACCGGCTTCaa > 1:798812/1‑76 (MQ=255) acccGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAAc < 1:7009822/74‑1 (MQ=255) aaCCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAAc < 1:6665949/76‑1 (MQ=255) gCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCg > 1:5785032/1‑76 (MQ=255) cTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAAAGGCGa > 1:1403636/1‑76 (MQ=255) tCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGat < 2:1254667/76‑1 (MQ=255) tCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGat < 2:3346965/76‑1 (MQ=255) tCACCTGGGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGat < 1:1982735/76‑1 (MQ=255) cAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGata < 1:5049553/76‑1 (MQ=255) cAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGata > 1:4010157/1‑76 (MQ=255) cAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGata > 2:1737180/1‑76 (MQ=255) cAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGata < 1:1011573/76‑1 (MQ=255) aGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAAAGGCGgtat > 1:494033/1‑76 (MQ=255) tGCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCa < 1:3586265/76‑1 (MQ=255) gCGAAGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCAt < 2:3603367/76‑1 (MQ=255) aacccAGCCGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGgt < 1:3992205/73‑1 (MQ=255) aGCCAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGgtg < 1:1635321/76‑1 (MQ=255) ccAGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGgtgtg < 1:712111/76‑1 (MQ=255) aGACGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGtt > 1:714814/1‑76 (MQ=255) gcGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTa < 1:3417141/75‑1 (MQ=255) aCGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTa < 1:261526/76‑1 (MQ=255) aCGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTa < 1:7483594/76‑1 (MQ=255) cGCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTAt < 1:1582561/76‑1 (MQ=255) gCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATc < 1:1283684/76‑1 (MQ=255) gCACGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATc < 2:4848553/76‑1 (MQ=255) ccGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt < 2:6481659/75‑1 (MQ=255) ccGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt < 2:2656183/75‑1 (MQ=255) aCGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCCTGGGGTGTTTATCtt > 2:5604487/1‑76 (MQ=255) aCGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt < 2:5343336/76‑1 (MQ=255) aCGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt < 2:4544783/76‑1 (MQ=255) cGTTTTGGCCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTc < 2:2835042/76‑1 (MQ=255) cGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATTGTGTGTTTATCTTc > 1:4133174/1‑76 (MQ=255) cGTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTc < 2:4079923/76‑1 (MQ=255) gTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCttta > 2:6945911/1‑74 (MQ=255) gTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCa < 2:7549554/76‑1 (MQ=255) gTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCa > 2:728978/1‑76 (MQ=255) gTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCa > 1:1475545/1‑76 (MQ=255) gTTCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCa < 1:194049/76‑1 (MQ=255) ttCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCAt < 1:2317224/76‑1 (MQ=255) tCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTTAACGGCGATATCCATGGTGTGTTTATCTTCATc > 1:2172348/1‑76 (MQ=255) tCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGGTATCCCTGGTGTGTTTATCTTCAtt > 1:6585093/1‑75 (MQ=255) tCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATc < 1:4665013/76‑1 (MQ=255) tCTGACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATc < 1:6106038/76‑1 (MQ=255) gACCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccac < 1:423621/76‑1 (MQ=255) aCCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccacc < 2:4997262/76‑1 (MQ=255) aCCGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccacc < 2:2130086/76‑1 (MQ=255) ccGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCCTGGGGGGTTTATCTTCATccacca > 1:7597250/1‑76 (MQ=255) ccGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccacca < 2:3559829/76‑1 (MQ=255) ccGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATATTCATccacca > 1:939110/1‑76 (MQ=255) cGTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCcaccac > 2:3774887/1‑76 (MQ=255) gTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCCCCACg > 1:1677133/1‑76 (MQ=255) gTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACg < 1:4337624/76‑1 (MQ=255) gTTACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACg < 2:516388/76‑1 (MQ=255) tACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGAt < 1:3018371/76‑1 (MQ=255) tACGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGGGTGTTTATCTTCATTCACCACGAt > 1:4507605/1‑76 (MQ=255) cGGGCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAg < 1:6319162/76‑1 (MQ=255) | GTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAG > REL606/3251732‑3251877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |