| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,495,556 | A→C | intergenic (‑96/+32) | yzgL ← / ← glgP | hypothetical protein; b3427_1/glycogen phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,495,556 | 0 | A | C | 99.1% | 914.7 / ‑2.0 | 213 | intergenic (‑96/+32) | yzgL/glgP | hypothetical protein; b3427_1/glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base C (114/97); total (114/99) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
Only 100 of 219 total aligned reads displayed.
TGGCGAAATTATACTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGACCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGT > REL606/3495483‑3495628 | tGGCGAAATTATACTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGccc < 1:1781757/76‑1 (MQ=255) gCGAAATTATACTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCt > 2:3476655/1‑76 (MQ=255) cGAAATTATACTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCtt > 1:2477321/1‑76 (MQ=255) cGAAATGATTCTCCCCGAGTCCCCGTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCtt < 2:7471276/76‑1 (MQ=255) gAAATTATCCTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCttt < 1:3022814/76‑1 (MQ=255) gAAATTATACTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCttt > 2:394140/1‑76 (MQ=255) gAAATTATACTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCttt < 2:2537345/76‑1 (MQ=255) aTTATACTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGc < 1:1469500/76‑1 (MQ=255) tataCTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGccc > 2:850678/1‑76 (MQ=255) tataCTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGccc < 1:3703072/76‑1 (MQ=255) tataCTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGccc > 1:802942/1‑76 (MQ=255) tataCTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGccc > 1:3018072/1‑76 (MQ=255) tataCTCCCCGAGTCCCCTTGCCCATTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGccc > 2:6154862/1‑76 (MQ=255) atccTCCCCGAGTCCCCTTGCCCCTGCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGcccc < 2:2437909/73‑1 (MQ=255) ataCTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGcccc > 1:2360323/1‑76 (MQ=255) ataCTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGcccc > 2:505529/1‑76 (MQ=255) ataCTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGcccc < 1:3369364/76‑1 (MQ=255) ccTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGt < 1:2892064/75‑1 (MQ=255) aCTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGt > 1:1109762/1‑76 (MQ=255) ccccccGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGtt < 1:1081236/74‑1 (MQ=255) cTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGtt < 2:3279514/76‑1 (MQ=255) cTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGtt < 1:2731239/76‑1 (MQ=255) cTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGtt > 2:3181915/1‑76 (MQ=255) cTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGtt < 2:5606528/76‑1 (MQ=255) tCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTc > 2:5408058/1‑76 (MQ=255) ccccGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCt < 2:4026379/76‑1 (MQ=255) ccccGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCt > 1:1210629/1‑76 (MQ=255) ccccGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCt > 1:6354307/1‑76 (MQ=255) ccccGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCt > 2:1462560/1‑76 (MQ=255) cccGATTCCCCTTGCCCCTTCGGGACACTTTTCCAAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCta < 2:5776832/76‑1 (MQ=255) cccGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGTGAAAAAAACGGGCCCCTTTGGCCCCGTTCta < 2:5916319/76‑1 (MQ=255) cGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtctt > 2:6521976/1‑76 (MQ=255) cGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtatt > 2:2839730/1‑76 (MQ=255) cGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtatt > 1:3533317/1‑76 (MQ=255) gAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtattt < 2:4955168/76‑1 (MQ=255) gAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtattt < 2:2523844/76‑1 (MQ=255) gAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtattt > 1:2220613/1‑76 (MQ=255) gAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtattt < 2:4804069/76‑1 (MQ=255) gAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtattt > 1:6269357/1‑76 (MQ=255) gAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtattt > 2:5243702/1‑76 (MQ=255) gAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtattt < 1:4575598/76‑1 (MQ=255) aGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtattta > 1:1611965/1‑76 (MQ=255) gTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCtatttat < 2:6830454/76‑1 (MQ=255) ccccTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATttatta > 1:7305467/1‑76 (MQ=255) ccccTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATttatta < 2:2090565/76‑1 (MQ=255) cccTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAg < 1:2326737/76‑1 (MQ=255) cccTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAg < 2:5205904/76‑1 (MQ=255) ccTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGt < 2:4581654/76‑1 (MQ=255) ccTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGt > 1:7343303/1‑76 (MQ=255) cTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCACCTTTGGCCCCGTTCTATTTATTAGTg > 2:3804757/1‑76 (MQ=255) ttGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGa > 1:3393801/1‑76 (MQ=255) ttGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGa > 2:2612898/1‑76 (MQ=255) gCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGaaa > 2:1976079/1‑76 (MQ=255) gCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGaaa > 1:6743780/1‑76 (MQ=255) gCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGaaa > 2:3025577/1‑76 (MQ=255) cccTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAAtt < 1:5386916/76‑1 (MQ=255) ccTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTa < 2:6298657/76‑1 (MQ=255) cTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTAc > 2:3316942/1‑76 (MQ=255) ttCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACa < 1:2141186/76‑1 (MQ=255) ttCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACa > 2:5934175/1‑76 (MQ=255) cTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAAt < 1:3803366/76‑1 (MQ=255) cTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAAt > 2:1059966/1‑76 (MQ=255) tGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAAtc > 1:6012855/1‑76 (MQ=255) ggACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAAtct < 2:408627/76‑1 (MQ=255) ggACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAAtct > 1:5631178/1‑76 (MQ=255) ggACACTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAAtct > 2:1588388/1‑76 (MQ=255) acacTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCa < 2:1720149/76‑1 (MQ=255) acacTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCa > 2:2729651/1‑76 (MQ=255) acacTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCa < 1:3310235/76‑1 (MQ=255) acTTTTCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCAcc < 2:5516091/76‑1 (MQ=255) ttttCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCgg < 2:5122316/76‑1 (MQ=255) tttCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGa > 2:4926615/1‑76 (MQ=255) tttCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGa < 2:1597317/76‑1 (MQ=255) ttCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGAt < 2:4457524/76‑1 (MQ=255) ttCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGAt < 2:1222883/76‑1 (MQ=255) tCCGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATc < 2:3373022/76‑1 (MQ=255) cGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCaa > 2:1814903/1‑76 (MQ=255) cGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCaa > 1:3201190/1‑76 (MQ=255) cGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCaa > 1:646302/1‑76 (MQ=255) cGAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCaa > 1:6943755/1‑76 (MQ=255) gAAATGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAat > 1:5578098/1‑76 (MQ=255) aaaTGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAata > 2:650982/1‑76 (MQ=255) aaTGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAatat < 2:6640629/76‑1 (MQ=255) aaTGATGGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAatat < 1:5117761/76‑1 (MQ=255) atgatgGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAacatc > 2:7644932/1‑75 (MQ=255) atgatgGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATg > 1:4679155/1‑76 (MQ=255) tgatgGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGc > 2:754225/1‑76 (MQ=255) gatgGCGAAAAAACCGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGcc < 1:5815895/76‑1 (MQ=255) gatgGCGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGcc > 1:7107803/1‑76 (MQ=255) tgGCAAAAAAAACGGGACCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAg < 1:4250073/76‑1 (MQ=255) cGAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGata < 1:4348587/76‑1 (MQ=255) gAAAAAAACGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGatat < 2:294843/76‑1 (MQ=255) aaaaaaaCGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATg > 2:1150150/1‑76 (MQ=255) aaaaCGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATc < 2:4697037/76‑1 (MQ=255) aaaCGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCg > 2:291595/1‑76 (MQ=255) aaaCGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCg > 2:7206849/1‑76 (MQ=255) aaaCGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCg < 1:6539776/76‑1 (MQ=255) aaCGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCgg < 1:7581760/76‑1 (MQ=255) aaCGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCgg > 1:7455353/1‑76 (MQ=255) cGGGCCCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCg > 2:1008289/1‑76 (MQ=255) | TGGCGAAATTATACTCCCCGAGTCCCCTTGCCCCTTCTGGACACTTTTCCGAAATGATGGCGAAAAAAACGGGACCCTTTGGCCCCGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGT > REL606/3495483‑3495628 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |