| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,131,458 | 0 | A | . | 99.0% | 481.9 / ‑1.5 | 96 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (76/19); total (77/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01 | |||||||||||
Only 100 of 145 total aligned reads displayed.
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533 | aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCa < 1:3689277/76‑1 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaa < 1:2622638/76‑1 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaa > 1:7059994/1‑76 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaa > 1:8305959/1‑76 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaa < 1:4035631/76‑1 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaa < 1:3746342/76‑1 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAGAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaa > 1:7863706/1‑76 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaa < 1:5348447/76‑1 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaa < 2:7964621/76‑1 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaa < 1:3987330/76‑1 (MQ=255) tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaa > 1:3149259/1‑76 (MQ=255) tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaa < 1:5211860/76‑1 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaaac > 2:5764755/1‑75 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaaac > 2:2621159/1‑75 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaaac < 1:7049791/76‑2 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaaac < 2:1465668/76‑2 (MQ=255) gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGACaaaaaaaaag > 2:633806/1‑75 (MQ=255) tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaaacgg < 1:5591711/76‑4 (MQ=255) tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 1:756465/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:2322721/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:5451966/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt < 2:7240421/76‑1 (MQ=255) tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 2:1115042/1‑76 (MQ=255) tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 1:112085/1‑76 (MQ=255) tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 1:1475652/1‑76 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 2:140302/1‑76 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:1182578/1‑76 (MQ=255) aaaTGTCGAGATAACAATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc < 1:4854686/76‑1 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 2:2268715/1‑76 (MQ=255) tGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑gg < 1:363159/76‑1 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGa > 1:2210821/1‑76 (MQ=255) tCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAg > 1:2238312/1‑76 (MQ=255) tCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAg < 1:4772940/76‑1 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 1:843993/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 2:6637091/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 2:5627239/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 1:3060452/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 1:1990843/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑‑AAAAAAACGGCCTCCC‑GGAggg > 1:3284837/1‑76 (MQ=255) gagaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑‑AAAAAAACGGCCTCCC‑GGAGGG‑a > 2:3926284/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a > 2:8247817/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a < 2:686758/76‑1 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a > 1:1247298/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a < 1:406391/76‑1 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a < 2:3013820/76‑1 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a > 1:8146209/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a < 2:2847852/76‑1 (MQ=255) gaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑aa > 2:5194685/1‑76 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAg < 1:2443963/76‑1 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAg > 2:1914898/1‑76 (MQ=255) tAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGc < 1:4596924/76‑1 (MQ=255) tAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGc > 2:589459/1‑76 (MQ=255) tAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGc > 1:498055/1‑76 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGcc > 2:3128894/1‑76 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGcc > 1:285258/1‑76 (MQ=255) aCGATCACAAAAACGACAATATGAAAATT‑AATAGAGGATTGAAAGGC‑AAAAAAAACGGCCCCCC‑GGAGGG‑AAGCCg < 2:8359786/76‑1 (MQ=255) cGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGc > 2:7789015/1‑76 (MQ=255) gATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGca > 2:3406753/1‑76 (MQ=255) tCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGcaac > 2:5052256/1‑76 (MQ=255) cacaAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑‑AAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGcaacaa > 1:7573990/1‑76 (MQ=255) acaAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGcaacaa > 1:1097176/1‑76 (MQ=255) aaaaaaaCGAAAATAGAAAAATA‑AATAGAGGAAT‑AAAGG‑AAAAAAAAACCGCCCCCC‑GGGGGGAAAGCCGcaacaa < 2:5193815/74‑1 (MQ=255) aaaCGAAAATAGAAAAATA‑AATAGAGAAAT‑AAAAGAAAAAAAAAACGCCCCCCC‑GGAGGA‑AAGCCGCAACAAAGTg < 2:7201555/76‑1 (MQ=255) aaCGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTgc > 2:6393602/1‑76 (MQ=255) aCGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTgcg > 1:6424646/1‑76 (MQ=255) cGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTgcgc > 2:3799996/1‑76 (MQ=255) aCAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGt > 1:7761178/1‑76 (MQ=255) atGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGttt > 2:4786514/1‑76 (MQ=255) atGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGttt > 2:4698240/1‑76 (MQ=255) agAAAATT‑AA‑AAAAGAAAAAAAAGCAAAAAAAACCGGCCTCCC‑GGGGAA‑AAGCCGCAACAAAGTGCGCGTGGtttt < 2:1262410/75‑1 (MQ=255) aaaaaTTAAATAGAGAAAT‑AAAAGAAAAAAAAACCGCCCCCCG‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGtttt < 2:6978467/75‑1 (MQ=255) aaaaTA‑AATAGAGAAAAG‑AAGGGAAAAAAAAACGCCCCCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTAt < 1:8010048/76‑1 (MQ=255) aaaTT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATg > 2:7994111/1‑76 (MQ=255) aata‑aataGAGGAAAAAAAGG‑AAAAAAAAACGGCCCCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGc < 1:2566059/76‑1 (MQ=255) aaTT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGAGGTTTTATGc > 1:3561359/1‑76 (MQ=255) aaTT‑AATAGAGAAATGAAAGGC‑AAAAAAACCGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGc < 2:4118606/76‑1 (MQ=255) aaTT‑AATAGAGAAATAAAAGG‑AAAAAAAACCGGCCCCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGc < 1:860849/76‑1 (MQ=255) tt‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 1:1512133/1‑76 (MQ=255) aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 2:318859/1‑76 (MQ=255) aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 2:318858/1‑76 (MQ=255) aaTAGAGGAATGAAAGG‑AAAAAAAACCGGCCCCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa < 1:6118210/76‑1 (MQ=255) aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 1:730083/1‑76 (MQ=255) aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 2:7601401/1‑76 (MQ=255) aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 2:6943284/1‑76 (MQ=255) aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 2:2152534/1‑76 (MQ=255) tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg > 1:599440/1‑76 (MQ=255) aagagGAATAAAAGGC‑AAAAAAAACGGCCCCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg < 1:1989364/75‑1 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 1:2929859/1‑76 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 1:331941/1‑76 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 1:5962003/1‑76 (MQ=255) agagGAATGAAAGGC‑‑AAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcg > 2:1204678/1‑76 (MQ=255) ggAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg > 2:4976491/1‑76 (MQ=255) ggAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggc > 1:4603905/1‑76 (MQ=255) aaTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGGTTTATGCAGGATGCGGCGt > 2:7076138/1‑76 (MQ=39) agaaaagaaaaAAAAACCGCCCCCCG‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa < 2:3486585/75‑1 (MQ=25) aaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:2398472/1‑76 (MQ=255) aacgCAAAAAAAAGCGCCCCGCG‑GGGGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc < 2:5782211/73‑1 (MQ=21) aaaaaaaacCGGCCCCCG‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAtg < 1:1223109/76‑1 (MQ=38) aaaaaaaaaCGGCCCCCC‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAtg < 2:2112338/76‑1 (MQ=255) aaaaaaaaaCCGCCCCCCGGGAGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACatat < 1:5545923/76‑1 (MQ=37) | AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |