| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,722,532 | 0 | C | G | 34.9% | 111.7 / 33.4 | 63 | G104G (GGC→GGG) | srlB | glucitol/sorbitol‑specific enzyme IIA component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (7/34); new base G (22/0); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.96e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 191 total aligned reads displayed.
CGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGT > REL606/2722457‑2722606 | gAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGcc > 2:5455350/1‑76 (MQ=255) aaCTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGgcc > 1:7206991/1‑76 (MQ=255) aaCTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGgcc > 1:3994362/1‑76 (MQ=255) aCTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGgccc > 1:5911427/1‑75 (MQ=255) cTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTg < 1:8189296/76‑1 (MQ=255) cTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTg < 2:6875800/76‑1 (MQ=255) tGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGt < 2:4965079/76‑1 (MQ=255) tGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGt < 1:6045197/76‑1 (MQ=255) gggTTATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTa < 1:1442720/76‑1 (MQ=255) gggTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCCGTa > 2:4326834/1‑76 (MQ=255) gggTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTa < 1:1148733/76‑1 (MQ=255) gggTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTa < 2:3404632/76‑1 (MQ=255) gggTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTa < 1:2525118/76‑1 (MQ=255) ggTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTggat > 1:3692903/1‑73 (MQ=255) ggTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTAc < 1:7934902/76‑1 (MQ=255) gTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTGcc > 1:6488411/1‑76 (MQ=255) cATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCCGTGCCcg > 1:7270314/1‑76 (MQ=255) aTGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGGAcccca > 1:4126183/1‑74 (MQ=255) aTGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGGACCcgc > 1:4924250/1‑75 (MQ=255) tGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTGcccccc > 1:3318755/1‑73 (MQ=255) tGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTACCcgac < 2:2992129/76‑1 (MQ=255) cccccTGCTTTTCGATGGTTAAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGCCCCTGTCCCCGACGat < 2:2570816/74‑1 (MQ=255) cACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTTCCcgccgac > 2:111769/1‑75 (MQ=255) cACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCCGTACCCGACGat > 1:3198022/1‑76 (MQ=255) aCCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTGCCCGACGata > 1:7438730/1‑76 (MQ=255) cTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCCGCACCCCACGATATcg > 1:6784856/1‑76 (MQ=255) cTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTACCCGACGATATcg < 1:3911932/76‑1 (MQ=255) gctcTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATcgcg < 1:709858/73‑1 (MQ=255) cTTCGTTGGTTAAACCGAACGGGAATTT‑CCGGGCCCTGTCCATGGGGCAGGCCCTGTGCCCGACGATATCGCGCCg < 2:2826720/76‑1 (MQ=255) cTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCCGTACCCCACGCTAACGCGCCg > 1:4663629/1‑76 (MQ=255) ttCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTACCCGCCGATATCTCGcccg > 2:2330634/1‑74 (MQ=255) tcaaTGGTTTAAACGAAGCGAAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTACCCGACGATATCGCGCCggg < 1:5817927/73‑1 (MQ=255) tCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCCGTACCCGACGATAATGCGCCggg > 1:5851940/1‑76 (MQ=255) tCGATGGTTAAAACGAAGCGAAATTT‑CCGGGCACTGTCGATGTGGCAGCCCCTGTACCCGACGATATCGCGCCggg < 2:5890870/76‑1 (MQ=255) cGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTTCCCGACGATATCGCGCCcggg > 1:5082354/1‑75 (MQ=255) cGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTACCCCACGATATCGCGCCcggg > 2:2963182/1‑75 (MQ=255) gagggTATAAACGAGGCGAAATTT‑CGGGGCACTGTCCATGTGGCGGGCCCTGTACCCGACGATATCGCGCCGGGAt < 2:1546329/73‑1 (MQ=255) gATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCCCCGGGAt < 2:7553781/76‑1 (MQ=255) ttGGTTTAAAGGAAGCGGAATTT‑CGGGGCCCGGCCCATGTGGCAGTCCCTGTACCCGACGATATCGCGCAGGGATc < 1:1240117/75‑1 (MQ=255) aTGGTTTAAACGAACCGGACTTC‑CCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATc < 2:7679791/76‑1 (MQ=255) tGGTTTAAACAAAGCGGAATTC‑CCGGGCCCTGTCCATGTGGCGGCCCCGGTACCCGACGATATCGCGCCGGGATCg < 1:7857452/76‑1 (MQ=255) tGGTTAAAAGAAAGCGGATTTC‑CCGGTCACTGTCCATGTGGCAGGCCTTGCCCCCGACGATATCGCGCCGGGATCg < 2:6992563/76‑1 (MQ=255) gttttAAACGAAGGGGAATTT‑CGGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCgg < 2:5686538/74‑1 (MQ=255) ggTTTAAACGAAGCGGATTTT‑CGGGGCACTGCCCATGTGGCAGGCCCTGTACCCGACGATATCGCGCCGGGATCgg < 2:3644520/76‑1 (MQ=255) gTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTACCCCACGATATTGCGCCGGGGTCggg > 1:7728085/1‑75 (MQ=255) tttAAACGAGGCGAATTTT‑CGGGGCACTGTCCATGGGGCAGCCCCTGTCCCCGACGATATCGCGCCGGGATCGGtt < 2:4377914/76‑1 (MQ=255) tttAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTACCCGACGATATCGCGCCGGGGTCGGtt > 2:7627298/1‑76 (MQ=255) tttAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCCGTCCCCCACGATAACGCGCCCGGGTCgggt > 2:4780816/1‑74 (MQ=255) tttAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCCATACCACACGACAGCGGGCCGGGGTCggct > 1:2669630/1‑74 (MQ=255) ttaaaaCGAGGCGGAATTG‑CGGGGCACTGCCCATGTGGCAGGCCCTGTACCCGACGATATCGCGCCGGGATCGGtt < 2:1149367/73‑1 (MQ=255) ttaaaaCGAAGCGGTATTT‑CGGGGCCCTGTCGATGTGGCAGGCCTTGTCCCCGACGATATCGCGCCGGGATCGGtt < 1:2873669/73‑1 (MQ=255) ttAAACGAAGCGGATTTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTACCCGACGATATCGCGCCGGGATCGGttt < 2:4603725/76‑1 (MQ=255) ttAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTACCCGACGATATCGCGCCGGGGCCGGttt > 2:3725852/1‑76 (MQ=255) ttAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCCGTTCCCCGCGCGATCGCGCCGGGAGCggggt > 1:6540651/1‑73 (MQ=255) ttAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCCGTACCCGACGATATCGGGCCGCGGCCGGttt > 2:6029896/1‑76 (MQ=255) taaaaCGAAGCGGATTTC‑CCGGGCATTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGttt < 2:8141605/74‑1 (MQ=255) tAACCGAAGCGGATTTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGtttt < 1:7357690/76‑1 (MQ=255) tAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCCGTACCCGACGACATCACGCCGGGGTCGGtttt > 1:2088765/1‑76 (MQ=255) aaaaCGAAGCGGATTTT‑CCGGGCACTGTCCATGTGGCGGGCCCGGTCCCCGACGATATCGCGCCGGGATCGGtttt < 1:2366022/75‑1 (MQ=255) aaaGGATGCGGATTTC‑CCGGGCCCTGCCAATGGGGCAGCCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTg < 1:2389350/76‑1 (MQ=255) aaCGAGGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGCCCCTGTACCCGACGATATCGCGCCGGGATCGGTTTTGa < 2:2258219/76‑1 (MQ=255) cGAACCGGATTTT‑CGGGGCACTGCCCATGGGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAg < 1:5357072/76‑1 (MQ=255) aaGGGGAGTTC‑CCGGGCACTGTCCATGTGGCAGGCCCTGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGtt < 1:5743686/76‑1 (MQ=255) gCGGATTTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTg < 2:3316380/76‑1 (MQ=255) cGGAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTACCCGCCGATAACGCGGCCGGGTCGGGTTTTGAGtttta > 2:4366758/1‑74 (MQ=255) ggAATTT‑CCGGGCACTGTCCATGTGGCAGGGCCTGTACCCGACGATATCGCGCCGGGGGCGGGTTTGTAGTTTTaa > 1:6719227/1‑76 (MQ=255) cgAATTTCCCGGGCCTTGCCCGTGTGGCAGGCCCTTTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGa < 1:4882506/75‑1 (MQ=255) ttt‑CCGGGCACTGTCCATGTGGCAGGCCCTGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTg < 2:1883199/76‑1 (MQ=255) ttcccGGGCACTGTCCATGTGGCAGGCCCTGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTg < 2:7731386/73‑1 (MQ=255) tt‑CCGGGCACTGTCCATGTGGCAGGCCCCCTACCCGGCGGTATCGCGCCGGGGTGGGGTTTTGAGTTTTAATTTGt > 1:5888982/1‑76 (MQ=255) t‑CCGGGCACTGTCCATTGGGCAGGCCCTGACCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGtt < 2:3175691/76‑1 (MQ=255) gggCACTGTCCATGTGGCAGGGCCTGTAACCGACGGTATCGCGCCGGGGTCGGTTTTGAAGTTTGAATCTGTTTAg > 2:8038567/1‑76 (MQ=255) gCACTGTCCATGTGGCAGGGCCCCTACCCGACGATATTCCGCCGGGGACGGGTTTGGAGTTTGTATCTGGTCAggg > 2:417760/1‑75 (MQ=255) gCACTGCCCATGTGGCAGCCCCGGCCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGa < 2:6769743/76‑1 (MQ=255) cccTGTCCATGGGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAg < 1:5168793/74‑1 (MQ=255) aCTGTCCATGTGGCAGGGCCCGTACCCCCCGATATCGCGCCGGGGTCGGTTTTTTAGGTTTAATTTTTTAAGGAga > 2:2534007/1‑75 (MQ=255) ctttCCATGTGGCAGCCCCTGTCCCCGACGATATCGCGCCGGGAGCGGTTTTGAAGTTTGAATCTGTTAAGGAGTa < 2:6462409/73‑1 (MQ=255) cTGTCGATGGGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTa < 1:2272231/76‑1 (MQ=255) cTGTCCGTGTGGCCGCCCGTGCACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTa < 2:2624090/76‑1 (MQ=255) cTGTCCATGTGGCAGGGCCTGTTCCCGACGACATCGCCCCCGGGACGGGTTTTTAGTTTGAATCTCTTAAAGAGTa > 2:1623116/1‑76 (MQ=255) cTGTCCATGTGGCAGGGCCTCTTCCCGACGCGATCACGCCGGGGTCGGGGTTGTAGTTTGAATCTCTTTAGGAgga > 2:3481428/1‑74 (MQ=255) cTGTCCATGTGGCAGGCCCGGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTa < 2:8366079/76‑1 (MQ=255) cTGTCCAGGTGCCAGCCCCGGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTa < 2:2216605/76‑1 (MQ=255) tGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTaa < 2:7998971/76‑1 (MQ=255) tGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTaa < 1:4464265/76‑1 (MQ=255) tGTCCAGGTGGCAGCCCCGTTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTaa < 2:3610422/76‑1 (MQ=255) tCCATGTGGCAGGCCGTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTaaaa < 1:6211957/76‑1 (MQ=255) tCCATGGGGCAGGCCCTTTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTaaaa < 1:6937565/76‑1 (MQ=255) accATGGGGCGGGCCCTGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTaaaa < 1:6108893/75‑1 (MQ=255) ctaTGTGCCAGGCTTTGTGCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTaaaaa < 1:1913998/74‑1 (MQ=255) ccATGTGGCAGGGCCCGTACCCGACGATATCGCGCCGGGGGCGGGTTTGAAGGTTGAATCTGTTTAGGAGGaaaaa > 2:494786/1‑76 (MQ=255) cgtgtgGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTaaaaaa < 2:7256058/74‑1 (MQ=255) tgtgGCAGGCCTTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATg < 1:7439745/76‑1 (MQ=255) tgtgGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATg < 1:1169451/76‑1 (MQ=255) tgtgGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATg < 1:3475663/76‑1 (MQ=255) gCAGCCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATc < 2:6375431/76‑1 (MQ=255) cAGGCCCTGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCa > 2:3494907/1‑76 (MQ=255) ggCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAgg < 1:3797787/76‑1 (MQ=255) ggCCCTGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAgg < 2:6542672/76‑1 (MQ=255) gCCCTGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGt > 2:4752392/1‑76 (MQ=255) | CGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTT‑CCGGGCACTGTCCATGTGGCAGGCCCTGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGT > REL606/2722457‑2722606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |