| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 955,035 | 0 | A | C | 35.7% | 301.4 / 14.0 | 141 | T192P (ACC→CCC) | lolA | outer‑membrane lipoprotein carrier protein precursor |
| Reads supporting (aligned to +/- strand): ref base A (72/18); new base C (0/50); total (72/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-23 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 203 total aligned reads displayed.
CAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTT > REL606/954960‑955110 | cAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCa > 2:7401220/1‑76 (MQ=255) aGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCAc < 1:6608041/76‑1 (MQ=255) aCGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCAcccc > 1:1018531/1‑76 (MQ=255) aCGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCAcccc < 2:2544140/76‑1 (MQ=255) cGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccg > 2:7792438/1‑76 (MQ=255) gATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccgc > 1:3281692/1‑76 (MQ=255) aTCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccgcc > 2:5328319/1‑76 (MQ=255) tCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccgccg > 1:1518505/1‑76 (MQ=255) cgcgcAGCATTTATCAACTGAAACCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTCCCCCCCGCCGCa < 2:2853426/75‑1 (MQ=255) cgcAGCAGTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAg < 2:5114539/76‑1 (MQ=255) cagcagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGc > 1:137394/1‑76 (MQ=255) catttATCAACTGAAACCCCAGCAAAATGGGGCTGTGGATGCAGCAAAATTTCCCTTCCCCCCGCCGCAAGGCGTc < 2:2296277/73‑1 (MQ=255) cagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTc < 2:1305683/76‑1 (MQ=255) aagTTATCAACTAAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTc < 2:5742482/75‑1 (MQ=255) agTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCa < 2:5005055/76‑1 (MQ=255) agTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCa < 2:6265128/76‑1 (MQ=255) agTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAAATTACCTTCACCCCGCCGCAAAGCGTCa > 2:6310209/1‑76 (MQ=255) gTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCAc < 1:233950/76‑1 (MQ=255) gTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCAc > 1:776793/1‑76 (MQ=255) ttATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACg > 2:7209115/1‑76 (MQ=255) aTCAACTAAAACCCCAGCAAAATGGGTCTGTGGATGCAGCAAATTTTCCCTCCCCCCCGCCGCAAGGCGTCACGGt < 2:2591983/76‑1 (MQ=255) aTCAACGGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGt < 2:3248551/76‑1 (MQ=255) tCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTa < 2:6025474/76‑1 (MQ=255) cAACTGAAACCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAg < 1:7585811/76‑1 (MQ=255) cAACAGAAACCCCAGCAAAGTGGGGCGGTGGATGCAGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAg < 1:7102849/76‑1 (MQ=255) aCTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAgat < 1:432194/76‑1 (MQ=255) aCTGAAATCCCAGCAAAAGGGGGCTGGGGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAgat < 1:698607/76‑1 (MQ=255) ctaaaaCCCGAGAAAAATGGGTCTGTGGAGGAAGCAAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAgatg < 1:4770538/73‑1 (MQ=255) taaaaCCCGAGTAAAAGGGGCTTTTGGTTGCAACGAATTTTGCCTTCGCCCCGCCGCAAGGCGTCACGGTAgatga < 2:7986092/74‑1 (MQ=255) tGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAgatga < 2:4170260/76‑1 (MQ=255) gAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCAAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAgatgat < 1:2141072/76‑1 (MQ=255) aaaTCCCAGCAAAATGGGGCTGTGGAGGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATc < 1:1767590/76‑1 (MQ=255) aaccccAGAAAAAGGGGGCTGTGGATGCAGCGAATTTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCa < 1:4808224/73‑1 (MQ=255) aaTCCCAGAAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCa < 2:5715436/76‑1 (MQ=255) accccAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCaa < 1:8113428/74‑1 (MQ=255) aTCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCaa < 2:4858690/76‑1 (MQ=255) aTCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCaa < 1:1320759/76‑1 (MQ=255) aTCCAAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCaa < 2:5394654/76‑1 (MQ=255) cccAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACg > 2:5561095/1‑76 (MQ=255) cccAGAAAAATGGGGCTGTGGATGCAGGAAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACg < 2:2747326/76‑1 (MQ=255) ccAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGt > 2:8119457/1‑76 (MQ=255) ggaaaaaGGGGGCTGTGGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTaa < 1:4595784/73‑1 (MQ=255) agaaaaaTGGGCCTGGGGATGCAGCAAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTaa < 1:891730/73‑1 (MQ=255) gCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAg > 1:3715429/1‑76 (MQ=255) acAAAATGGGGCTGTGAATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAg < 2:6615733/75‑1 (MQ=255) aaaaaTGGGCCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGt < 1:4743707/75‑1 (MQ=255) aaaaaGGGGGCTGGGGATGCAGCGAAATTTCCCTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGt < 1:5608319/75‑1 (MQ=255) aaaaTGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTTACGGTAGATGATCAACGTAAGTa > 1:8282662/1‑76 (MQ=255) aaaaTGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCCACGTCAGTa > 1:2694409/1‑76 (MQ=255) aaaaTGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTa > 1:7982753/1‑76 (MQ=255) aaaaGGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTa < 2:3973713/76‑1 (MQ=255) aaaGGGGGCTGGGGAGGCAGCAAATTTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTag < 1:4516395/76‑1 (MQ=255) aaTGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTaga > 2:6119046/1‑76 (MQ=255) tGGGGCTGTGGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAgg < 2:1482735/76‑1 (MQ=255) tGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAgg > 2:4326588/1‑76 (MQ=255) tGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAgg > 2:363097/1‑76 (MQ=255) tGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAgg > 2:2643303/1‑76 (MQ=255) gggggCGTTGGAGGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAgg < 1:6392993/75‑1 (MQ=255) ggggCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGc < 1:87349/76‑1 (MQ=255) ggggCTGTGGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGc < 1:7193316/76‑1 (MQ=255) ggggCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGc > 1:4258041/1‑76 (MQ=255) gggTTGTGTATGCAGCGAATTTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCa < 2:5952631/76‑1 (MQ=255) gggCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCa < 2:3899610/76‑1 (MQ=255) ggCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCAc > 2:2286645/1‑76 (MQ=255) cggtgGAGGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCt < 2:5058356/74‑1 (MQ=255) cTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCt > 2:3543176/1‑76 (MQ=255) tgtgGATGCAGCGAATTTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtg < 1:4691390/76‑1 (MQ=255) tgtgGATGCAGCGAAATTTCCCTTCGCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtg < 2:542090/76‑1 (MQ=255) tgtgGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtg < 1:2560836/76‑1 (MQ=255) tgtgGAGGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtg < 2:171191/76‑1 (MQ=255) ggtgGATGCGGCAAAATTACCCTACCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCGCCtg < 2:899460/75‑1 (MQ=255) gtgGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtga > 2:4485782/1‑76 (MQ=255) gtgGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtga > 1:2290161/1‑76 (MQ=255) gtgGATGCAGCAAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtga < 1:3616242/76‑1 (MQ=255) gtgGAGGCAGCAAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtga < 1:3113894/76‑1 (MQ=255) tgGATGCAGCGAATTTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgag < 1:6423856/76‑1 (MQ=255) tgGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgag > 1:5814427/1‑76 (MQ=255) gggATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgag < 2:4081359/75‑1 (MQ=255) gATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtg < 2:2078137/76‑1 (MQ=255) gATGCAGCGAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtg < 2:1698819/76‑1 (MQ=255) aTGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtga < 1:6509081/76‑1 (MQ=255) cAGCAAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCa < 1:8019811/76‑1 (MQ=255) aGCAAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCaa < 2:1114581/76‑1 (MQ=255) gCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAAt < 1:6883726/76‑1 (MQ=255) cGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATc > 2:6525485/1‑76 (MQ=255) cGAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATc < 1:8159960/76‑1 (MQ=255) gAATTTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCt < 1:5028818/76‑1 (MQ=255) gAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCt < 1:3921177/76‑1 (MQ=255) aattttCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTg < 2:4945203/73‑1 (MQ=255) aaaTTTCCCTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAGCGTAAGTAGAGGCACCTGAGTGAGCAATCTg < 2:6475369/76‑1 (MQ=255) aTTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTc < 2:7665819/76‑1 (MQ=255) tttCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCg < 1:618188/76‑1 (MQ=255) tttACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCg > 2:3772624/1‑76 (MQ=255) tACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCCACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCt > 1:3774775/1‑76 (MQ=255) tACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCt > 1:802770/1‑76 (MQ=255) aCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTc > 1:4829365/1‑76 (MQ=255) ccTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCg < 1:8342350/76‑1 (MQ=255) ccTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCg > 1:174108/1‑76 (MQ=255) cACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttt > 2:3044515/1‑76 (MQ=255) aCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAtttt > 2:2905132/1‑76 (MQ=255) | CAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTT > REL606/954960‑955110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |