BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	52,039	C→T	L26F (CTT→TTT)	kefC →	glutathione‑regulated potassium‑efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	52,039	0	C	T	100.0%	722.0 / NA	171	L26F (CTT→TTT)	kefC	glutathione‑regulated potassium‑efflux system protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (84/87); total (84/87)

Only 100 of 190 total aligned reads displayed.

GGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGA  >  REL606/51966‑52111

ggATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGtttt                                                                        >  1:5540547/1‑76 (MQ=255)

ggATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCCGGTTGTACCCCTTGAGGTAAGtttt                                                                        >  2:2452796/1‑76 (MQ=255)

 gATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGGACGTTTTg                                                                       >  2:6086709/1‑76 (MQ=255)

 gATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGAGCTGATTGTACCCCATGGGGTACGTTTTg                                                                       >  2:7806583/1‑76 (MQ=255)

   tAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGGACGTTTTggg                                                                     >  1:76593/1‑75 (MQ=255)

      ccATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTg                                                                  <  1:6135002/76‑1 (MQ=255)

       cATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTgg                                                                 <  1:3023493/76‑1 (MQ=255)

       cATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTgg                                                                 <  1:5934393/76‑1 (MQ=255)

       cATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGGTTTGGTCTgg                                                                 >  1:1088118/1‑76 (MQ=255)

        aTACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGGACGTTTTGGTCTtgg                                                                >  1:6494038/1‑76 (MQ=255)

         tACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGa                                                               <  2:1692360/76‑1 (MQ=255)

          aCGCTGCTTCCGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGAt                                                              >  1:7793996/1‑76 (MQ=255)

          aCGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGAt                                                              >  1:7725117/1‑76 (MQ=255)

          aCGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGAt                                                              <  2:4482164/76‑1 (MQ=255)

            gCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGGACGTTTTGGTATGGGAttg                                                            >  2:3499248/1‑74 (MQ=255)

              tGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGt                                                          <  2:556740/76‑1 (MQ=255)

              tGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGt                                                          <  1:4707595/76‑1 (MQ=255)

              tGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGt                                                          >  1:699666/1‑76 (MQ=255)

               taTTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTa                                                         <  2:4546771/75‑1 (MQ=255)

               gATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTa                                                         <  1:3406631/76‑1 (MQ=255)

               gATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTa                                                         >  2:4950774/1‑76 (MQ=255)

                aTTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTAc                                                        <  1:3817726/76‑1 (MQ=255)

                aTTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTAc                                                        <  1:285033/76‑1 (MQ=255)

                aTTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAACCATTGCGGTACGGTTTGGTCTGGGATCGGTAc                                                        >  1:4747142/1‑76 (MQ=255)

                 ttCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACt                                                       <  2:5135393/76‑1 (MQ=255)

                  tCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACtt                                                      <  2:3248541/76‑1 (MQ=255)

                  tCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACtt                                                      <  1:4601361/76‑1 (MQ=255)

                  tCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACtt                                                      <  2:2536883/76‑1 (MQ=255)

                   cAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTg                                                     <  1:7742661/76‑1 (MQ=255)

                   cAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTg                                                     <  2:7167996/76‑1 (MQ=255)

                   cAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTg                                                     >  1:1922575/1‑76 (MQ=255)

                   cAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTg                                                     <  1:115664/76‑1 (MQ=255)

                    aGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTgg                                                    >  1:7928761/1‑76 (MQ=255)

                    aGGCGCTGATTTAACTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTgg                                                    <  2:5402626/76‑1 (MQ=255)

                       cgcTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTa                                                 >  2:6656297/1‑76 (MQ=255)

                       cgcTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTa                                                 <  1:6718494/76‑1 (MQ=255)

                          tGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCt                                              >  1:1470837/1‑76 (MQ=255)

                          tGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCt                                              <  1:7567483/76‑1 (MQ=255)

                           gATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTg                                             <  2:4190276/76‑1 (MQ=255)

                           gATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTg                                             >  2:8327511/1‑76 (MQ=255)

                             tttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGAt                                           >  2:2202243/1‑76 (MQ=255)

                              ttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATc                                          >  1:7908722/1‑76 (MQ=255)

                              ttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATc                                          >  2:3986345/1‑76 (MQ=255)

                              ttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATc                                          <  2:2684463/76‑1 (MQ=255)

                              ttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATc                                          <  1:3307144/76‑1 (MQ=255)

                              ttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATc                                          >  2:7354806/1‑76 (MQ=255)

                               tATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCg                                         >  1:2233636/1‑76 (MQ=255)

                               tATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCg                                         <  1:6405702/76‑1 (MQ=255)

                               tATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCg                                         <  2:1943586/76‑1 (MQ=255)

                               tATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCg                                         <  1:3533662/76‑1 (MQ=255)

                               tATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCg                                         <  1:4220230/76‑1 (MQ=255)

                               tATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTAACTGATCg                                         >  2:5645911/1‑76 (MQ=255)

                                aTCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGc                                        <  2:4597640/76‑1 (MQ=255)

                                 tctcGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGcc                                       >  1:4121243/1‑76 (MQ=255)

                                    cGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGc                                    >  2:5857060/1‑76 (MQ=255)

                                      gTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTg                                  <  2:7638332/76‑1 (MQ=255)

                                      gTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTg                                  >  2:6944236/1‑76 (MQ=255)

                                       ttCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTgg                                 >  2:3349578/1‑75 (MQ=255)

                                         cGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat                               >  2:2891350/1‑76 (MQ=255)

                                         cGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat                               <  1:1446587/76‑1 (MQ=255)

                                         cGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat                               >  1:1328991/1‑76 (MQ=255)

                                          ggCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCatt                              <  1:2516989/76‑1 (MQ=255)

                                             aGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCattatt                           >  2:3212031/1‑76 (MQ=255)

                                                gcTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACGTGGCTACCTGATCGCCGGCTGCATTATTGGc                        <  2:6469355/76‑1 (MQ=255)

                                                 cTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGcc                       >  1:5582109/1‑76 (MQ=255)

                                                 cTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGcc                       >  1:2200316/1‑76 (MQ=255)

                                                  tGATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGccc                      >  1:6700282/1‑76 (MQ=255)

                                                   gATTGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTTGCCCg                     >  2:8255846/1‑76 (MQ=255)

                                                      tGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTgg                  <  2:7861976/76‑1 (MQ=255)

                                                      tGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTgg                  >  2:939524/1‑76 (MQ=255)

                                                      tGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTgg                  <  2:109598/76‑1 (MQ=255)

                                                      tGTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTgg                  >  1:1303252/1‑76 (MQ=255)

                                                       gTACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTggg                 >  1:4520303/1‑76 (MQ=255)

                                                        tACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTgggg                >  1:7167268/1‑76 (MQ=255)

                                                        tACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTgggg                <  2:893421/76‑1 (MQ=255)

                                                        tACCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTgggg                <  1:1793430/76‑1 (MQ=255)

                                                         aCCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTggggg               <  2:3644554/76‑1 (MQ=255)

                                                         aCCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTggggg               <  1:799384/76‑1 (MQ=255)

                                                         aCCCATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTggggg               >  1:1121770/1‑76 (MQ=255)

                                                           ccATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCt             <  1:995514/76‑1 (MQ=255)

                                                           ccATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCt             <  2:5661867/76‑1 (MQ=255)

                                                           ccATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCt             <  1:6850293/76‑1 (MQ=255)

                                                           ccATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCt             <  2:632089/76‑1 (MQ=255)

                                                            cATTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTg            >  1:7701355/1‑76 (MQ=255)

                                                             aTTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGGTGc           >  1:6971874/1‑76 (MQ=255)

                                                             aTTGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGGGGGGGCTGc           >  1:4866168/1‑76 (MQ=255)

                                                              ttGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCg          >  2:6788343/1‑76 (MQ=255)

                                                              ttGCGGTACGTTTTGGTCTGGAACGGGTCTTTGCCAACTTGAACGCGGGCTGGATTCTTGGCCCGTGGGGGCTgag          >  2:7413438/1‑74 (MQ=255)

                                                               tGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTTTTGGGCCGCGGGGGCGGCGa         >  2:3148814/1‑76 (MQ=255)

                                                               tGCGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGGGa         >  2:2383144/1‑76 (MQ=255)

                                                                 gggTACGTTTTGGTCTGGGATCGGTACTTGGCTCCCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACt       <  2:1232671/75‑1 (MQ=255)

                                                                 cGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCgcgc       >  2:7328376/1‑73 (MQ=255)

                                                                 cGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACt       <  1:5971656/76‑1 (MQ=255)

                                                                 cGGTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGGGGGGGGTGCggcc       >  1:1568268/1‑73 (MQ=255)

                                                                   gTACGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGGTGGGACCgg     >  2:1035155/1‑76 (MQ=255)

                                                                     aCGTTTTGGTCTGGGATCGGTACTTGGCTGCCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTg   <  1:3608239/76‑1 (MQ=255)

                                                                     aCGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGGCCGTGGGGGGCGGGACTtgtg   >  1:395794/1‑76 (MQ=255)

                                                                     aCGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTg   <  2:4893143/76‑1 (MQ=255)

                                                                      cGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGa  >  1:3795818/1‑76 (MQ=255)

                                                                      cGTTTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGa  <  1:2902575/76‑1 (MQ=255)

GGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGA  >  REL606/51966‑52111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`