BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,495,641	T→C	T799A (ACT→GCT)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,495,641	0	T	C	98.8%	1113.2 / 0.2	259	T799A (ACT→GCT)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base T (3/0); new base C (175/81); total (178/81)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 260 total aligned reads displayed.

CGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGTACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTC  >  REL606/3495568‑3495713

 gTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACg                                                                       >  2:1642473/1‑76 (MQ=255)

  ttCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACgg                                                                      >  2:5289654/1‑76 (MQ=255)

  ttCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACgg                                                                      >  1:267002/1‑76 (MQ=255)

   tCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGt                                                                     >  2:6214401/1‑76 (MQ=255)

   tCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGt                                                                     <  1:1607777/76‑1 (MQ=255)

    cTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGTACGGTc                                                                    >  1:343617/1‑76 (MQ=255)

    cTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTc                                                                    <  1:6246440/76‑1 (MQ=255)

    cTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTc                                                                    <  1:3041881/76‑1 (MQ=255)

     tatttattAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCa                                                                   <  2:4101237/76‑1 (MQ=255)

      atttattAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCag                                                                  >  1:3234002/1‑76 (MQ=255)

       tttattAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCaga                                                                 >  2:1712011/1‑76 (MQ=255)

        ttattaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaa                                                                <  2:6427701/76‑1 (MQ=255)

        ttattaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaa                                                                >  1:6612774/1‑76 (MQ=255)

        ttattaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaa                                                                >  1:1270151/1‑76 (MQ=255)

        ttattaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaa                                                                >  1:6731945/1‑76 (MQ=255)

         tattaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaag                                                               <  2:2553225/76‑1 (MQ=255)

         tattaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaag                                                               >  1:152540/1‑76 (MQ=255)

         tattaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaag                                                               >  2:3964752/1‑76 (MQ=255)

          attaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaaga                                                              >  2:4756723/1‑76 (MQ=255)

          attaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaaga                                                              >  2:3773800/1‑76 (MQ=255)

           ttaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaagag                                                             >  1:474786/1‑76 (MQ=255)

           ttaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaagag                                                             <  1:3925587/76‑1 (MQ=255)

           ttaGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaagag                                                             <  1:1892708/76‑1 (MQ=255)

             aGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCagaagagaa                                                           >  1:6352587/1‑76 (MQ=255)

               tGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGt                                                         <  2:7653809/76‑1 (MQ=255)

                 aaaTTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAg                                                       >  2:5018772/1‑76 (MQ=255)

                  aaTTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGc                                                      >  1:1759076/1‑76 (MQ=255)

                    ttACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGccc                                                    >  1:8183381/1‑76 (MQ=255)

                     tACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCa                                                   >  1:2182287/1‑76 (MQ=255)

                      aCAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCat                                                  >  1:8308128/1‑76 (MQ=255)

                      aCAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCat                                                  >  1:5975918/1‑76 (MQ=255)

                       cAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCata                                                 <  2:4622289/76‑1 (MQ=255)

                        aaTCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCatat                                                <  1:6228175/76‑1 (MQ=255)

                        aaTCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCatat                                                >  1:2378584/1‑76 (MQ=255)

                         aTCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATAtt                                               <  2:2845812/76‑1 (MQ=255)

                         aTCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATAtt                                               <  1:5275571/76‑1 (MQ=255)

                         aTCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATAtt                                               >  2:5506774/1‑76 (MQ=255)

                           ctcACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGTACGGTCAGAAGAGAAGTAGCCCATATTgg                                             >  1:6431582/1‑76 (MQ=255)

                           ctcACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTgg                                             <  2:1908006/76‑1 (MQ=255)

                            tcACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGc                                            >  1:1012662/1‑76 (MQ=255)

                            tcACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGc                                            <  2:1954514/76‑1 (MQ=255)

                            tcACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGc                                            >  2:7884455/1‑76 (MQ=255)

                             cACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCa                                           >  1:4578136/1‑76 (MQ=255)

                             cACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCa                                           <  1:6907211/76‑1 (MQ=255)

                               ccGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAAt                                         >  1:5799998/1‑76 (MQ=255)

                                cGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATg                                        >  2:1019583/1‑76 (MQ=255)

                                   aTCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTc                                     >  1:7972757/1‑76 (MQ=255)

                                    tCAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCa                                    <  1:7138640/76‑1 (MQ=255)

                                     cAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAg                                   >  2:2647418/1‑76 (MQ=255)

                                     cAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAg                                   >  2:3742666/1‑76 (MQ=255)

                                     cAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAg                                   >  2:883478/1‑76 (MQ=255)

                                     cAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAg                                   >  1:7544534/1‑76 (MQ=255)

                                     cAATATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAg                                   >  1:547999/1‑76 (MQ=255)

                                      aaTATGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGc                                  >  1:4876020/1‑76 (MQ=255)

                                       atatGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCa                                 <  1:4138696/76‑1 (MQ=255)

                                       atatGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCa                                 >  1:1174649/1‑76 (MQ=255)

                                        tatGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCAt                                >  2:3928085/1‑76 (MQ=255)

                                          tGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCg                              <  1:6136576/76‑1 (MQ=255)

                                          tGCCAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCg                              <  2:231862/76‑1 (MQ=255)

                                            ccAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCt                            >  1:1558610/1‑76 (MQ=255)

                                             cAGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCtt                           >  2:6984327/1‑76 (MQ=255)

                                              aGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCttt                          >  1:2033934/1‑76 (MQ=255)

                                              aGATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCttt                          >  2:3119400/1‑76 (MQ=255)

                                               gATATGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCtttt                         <  2:6651964/76‑1 (MQ=255)

                                                atatGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTg                        >  2:3313496/1‑76 (MQ=255)

                                                 tatGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGc                       <  2:3437351/76‑1 (MQ=255)

                                                  atGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCg                      >  1:2971596/1‑76 (MQ=255)

                                                  atGATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCg                      >  1:742800/1‑76 (MQ=255)

                                                    gATCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGt                    >  1:7734731/1‑76 (MQ=255)

                                                     aTCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTc                   <  1:4366646/76‑1 (MQ=255)

                                                      tCGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTcc                  >  2:112985/1‑76 (MQ=255)

                                                       cGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCa                 >  1:1741582/1‑76 (MQ=255)

                                                       cGGCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCa                 <  1:5532866/76‑1 (MQ=255)

                                                         gCGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCAct               >  2:5984990/1‑76 (MQ=255)

                                                          cGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCActc              >  2:276284/1‑76 (MQ=255)

                                                          cGTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCActc              >  2:7117523/1‑76 (MQ=255)

                                                           gTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCActct             >  2:6708517/1‑76 (MQ=255)

                                                           gTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCActct             >  2:7703066/1‑76 (MQ=255)

                                                           gTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCActct             >  1:3508711/1‑76 (MQ=255)

                                                           gTACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCActct             >  1:734899/1‑76 (MQ=255)

                                                            tACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCtt            >  1:1344443/1‑76 (MQ=255)

                                                            tACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCtt            >  2:5770030/1‑76 (MQ=255)

                                                            tACTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCtt            <  2:593453/76‑1 (MQ=255)

                                                             aCTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTc           >  2:560095/1‑76 (MQ=255)

                                                             aCTCTTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTc           >  1:7133000/1‑76 (MQ=255)

                                                              ctctTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTcc          >  2:4951287/1‑76 (MQ=255)

                                                               tctTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCt         <  2:7387404/76‑1 (MQ=255)

                                                                ctTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTg        >  1:4697084/1‑76 (MQ=255)

                                                                ctTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTg        >  1:5291624/1‑76 (MQ=255)

                                                                ctTTGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTg        <  1:8275242/76‑1 (MQ=255)

                                                                 tttGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGa       >  1:1981496/1‑76 (MQ=255)

                                                                  ttGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGaa      >  2:794164/1‑76 (MQ=255)

                                                                   tGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAg     >  1:6461687/1‑76 (MQ=255)

                                                                   tGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAg     >  2:6527572/1‑76 (MQ=255)

                                                                   tGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAg     <  1:718615/76‑1 (MQ=255)

                                                                   tGATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAg     <  1:4192746/76‑1 (MQ=255)

                                                                    gATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGc    >  1:910699/1‑76 (MQ=255)

                                                                    gATAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGc    >  1:7837501/1‑76 (MQ=255)

                                                                      tAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTc  >  1:3106491/1‑76 (MQ=255)

                                                                      tAGCACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTc  >  1:3121150/1‑76 (MQ=255)

CGTTCTATTTATTAGTGAAATTACAATCTCACCGGATCAATATGCCAGATATGATCGGCGTACTCTTTGATAGTACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTC  >  REL606/3495568‑3495713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`