| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,929,292 | A→C | D359A (GAC→GCC) | rho → | transcription termination factor Rho |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,929,292 | 0 | A | C | 99.3% | 1239.2 / ‑0.6 | 293 | D359A (GAC→GCC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (144/147); total (146/147) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01 | |||||||||||
Only 100 of 311 total aligned reads displayed.
TTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCT > REL606/3929219‑3929364 | ttAAAGGTACAGGCAACATGGTACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTa < 2:5055512/76‑1 (MQ=255) ttAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTa < 2:2325241/76‑1 (MQ=255) tAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTAc > 2:6686118/1‑76 (MQ=255) aaaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACa < 2:4283305/76‑1 (MQ=255) aaaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACa > 1:60740/1‑76 (MQ=255) aaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACaa > 2:1422534/1‑76 (MQ=255) aaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACaa < 1:738842/76‑1 (MQ=255) aaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACaa < 1:3562402/76‑1 (MQ=255) gTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCg > 1:3372562/1‑76 (MQ=255) tACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGt > 2:8178671/1‑76 (MQ=255) tACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGt > 1:5295065/1‑76 (MQ=255) gcAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGtt > 1:3256825/2‑76 (MQ=255) aGGCAACATGGAACTGCCCCTCTCTCGTAAGATCGCTGAAAAACGCGGCTTCCCGGCTATCGCCTACAACCGTTCt < 2:7189621/76‑1 (MQ=255) ggCAACCTGGAACTGCCCCCCTCTTCTAAAAACGCTTTAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTg < 2:1978497/76‑1 (MQ=255) ggCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTg < 1:1577464/76‑1 (MQ=255) cAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGt > 1:2022199/1‑76 (MQ=255) aaCATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTa > 1:8185079/1‑76 (MQ=255) aaCATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTa > 1:903326/1‑76 (MQ=255) aaCATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTa > 1:6237817/1‑76 (MQ=255) aTGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTAccc > 1:1221398/1‑76 (MQ=255) aTGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTAccc > 2:1282714/1‑76 (MQ=255) aTGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTAccc > 1:7976034/1‑76 (MQ=255) tGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCCTTCCGGTACCCg > 1:2796621/1‑76 (MQ=255) ggAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGt > 1:683693/1‑76 (MQ=255) aaCTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTaa > 2:4567016/1‑76 (MQ=255) aaCTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTaa < 1:1137518/76‑1 (MQ=255) aCTGCCCCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTaaa < 2:2960144/76‑1 (MQ=255) tGCCCCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaaga < 2:4920734/76‑1 (MQ=255) tGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaaga > 2:3264952/1‑76 (MQ=255) tGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaaga > 1:2322096/1‑76 (MQ=255) gccccTCTCTCTTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaagaa < 2:3960682/73‑1 (MQ=255) ccccTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaagaag < 1:6952981/74‑1 (MQ=255) cACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaagaag < 2:6862425/76‑1 (MQ=255) aCCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAagaaga > 2:5972587/1‑76 (MQ=255) aCCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAagaaga > 2:1670008/1‑76 (MQ=255) aCCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAagaaga > 2:8346320/1‑76 (MQ=255) ccTCTCTCGTAAGATCGCTGAAAAACGCGTCTCCCCGGCTATCGCCTCCAACCGTTCTGGTACCCGTAAAGAagag < 1:3811733/76‑1 (MQ=255) ctctctCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAgc < 2:3576911/76‑1 (MQ=255) tctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAgctgc > 2:5230106/1‑76 (MQ=255) ctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAgctgct > 1:2463712/1‑76 (MQ=255) ctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAgctgct > 2:3883472/1‑76 (MQ=255) ctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAgctgct > 1:3183001/1‑76 (MQ=255) tcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTc < 1:2903723/76‑1 (MQ=255) tcGTAAGACCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTc < 2:448277/76‑1 (MQ=255) gcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTc < 2:6560804/75‑1 (MQ=255) cGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCa > 1:7577256/1‑76 (MQ=255) cGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCCGCTCa > 1:7690775/1‑76 (MQ=255) cGTAAGAACGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCa < 1:3619029/76‑1 (MQ=255) gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc > 1:675382/1‑76 (MQ=255) gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc > 1:181404/1‑76 (MQ=255) gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc < 1:1921562/76‑1 (MQ=255) gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc < 1:3395045/76‑1 (MQ=255) gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc < 1:5748816/76‑1 (MQ=255) gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc > 1:5096555/1‑76 (MQ=255) tAAGATCGCTGAAAACCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACg < 1:4400084/76‑1 (MQ=255) tAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACg < 2:4674042/76‑1 (MQ=255) tAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACg < 2:2494517/76‑1 (MQ=255) aaGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGa < 2:800427/76‑1 (MQ=255) aaGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGa > 2:3399873/1‑76 (MQ=255) aaGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGa > 1:3994649/1‑76 (MQ=255) gATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACt < 2:3150726/76‑1 (MQ=255) tCGCTGAAAACCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCa < 2:6375935/76‑1 (MQ=255) tCGCTGAAAAACCCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCa < 1:5937020/76‑1 (MQ=255) gcggAAAAACGCGTCTTCCCGTCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAgg < 2:278837/73‑1 (MQ=255) gCTGAAAAACGCGTCTCCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAgg < 1:4676770/76‑1 (MQ=255) cTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGga > 2:7452827/1‑76 (MQ=255) cTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGga > 1:4137421/1‑76 (MQ=255) cTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCCTAAAGAAGAGCTGCTCACGACTCAGga > 1:4565080/1‑76 (MQ=255) gAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGgaag < 1:7832924/76‑1 (MQ=255) aaaaaaCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGgaag < 2:2852744/75‑1 (MQ=255) aaCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACt > 2:544694/1‑76 (MQ=255) aCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTg < 2:7043191/76‑1 (MQ=255) aCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTg < 2:2704145/76‑1 (MQ=255) cgcgTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGc > 1:488456/1‑76 (MQ=255) cgcgTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGc < 2:6919249/76‑1 (MQ=255) gcgTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCa < 2:4866847/76‑1 (MQ=255) cttCTTCCCGGTTATCTCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAg < 1:993543/74‑1 (MQ=255) cgTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAg < 2:5776664/76‑1 (MQ=255) cgTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAg < 2:8223250/76‑1 (MQ=255) gTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGa < 2:7325362/76‑1 (MQ=255) tCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaa < 1:6981214/76‑1 (MQ=255) tCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaa < 2:5563447/76‑1 (MQ=255) cTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaaa > 1:2097262/1‑76 (MQ=255) ttCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaaaa > 1:5662107/1‑76 (MQ=255) ttCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaaaa > 2:1060010/1‑76 (MQ=255) cccGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtg > 1:3130478/1‑76 (MQ=255) cgccTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgtg < 1:1282714/73‑1 (MQ=255) cGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgtg > 2:1169019/1‑76 (MQ=255) gCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGa > 2:6225534/1‑76 (MQ=255) gCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGa < 2:5640922/76‑1 (MQ=255) gCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGa > 1:3696120/1‑76 (MQ=255) ttATCGCTTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt < 2:7541736/75‑1 (MQ=255) cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt > 1:720439/1‑76 (MQ=255) cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt > 2:5902097/1‑76 (MQ=255) cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt > 2:2183193/1‑76 (MQ=255) cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt < 2:7642891/76‑1 (MQ=255) cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt < 1:4231122/76‑1 (MQ=255) cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt < 2:1581868/76‑1 (MQ=255) tATCGCCTACAACCGTTCTGGTGCCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATc < 1:5168828/76‑1 (MQ=255) tATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATc > 1:6508565/1‑76 (MQ=255) | TTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCT > REL606/3929219‑3929364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |