| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,108,360 | T→C | N48S (AAC→AGC) | metJ ← | transcriptional repressor protein MetJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,108,360 | 0 | T | C | 99.3% | 1146.5 / ‑4.0 | 278 | N48S (AAC→AGC) | metJ | transcriptional repressor protein MetJ |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (123/153); total (124/154) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 291 total aligned reads displayed.
CGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCT > REL606/4108287‑4108432 | cGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgctg > 1:4194667/1‑76 (MQ=255) ggCAAAGGATGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgctgg > 2:3382863/1‑75 (MQ=255) cAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTc < 2:3110197/76‑1 (MQ=255) cAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTc < 2:1998143/76‑1 (MQ=255) ggTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCt < 2:4136356/76‑1 (MQ=255) gTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTg < 2:4650592/76‑1 (MQ=255) ttGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgg < 2:5690760/76‑2 (MQ=255) ttGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTga > 2:5950157/1‑76 (MQ=255) ttGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTga < 2:5206931/76‑1 (MQ=255) tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCa < 2:2377576/70‑1 (MQ=255) tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgac > 1:4411473/1‑76 (MQ=255) tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgac < 1:2893310/76‑1 (MQ=255) tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgac < 1:7155967/76‑1 (MQ=255) tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgac > 1:7947410/1‑76 (MQ=255) gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgacg < 1:7652723/76‑1 (MQ=255) gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgacg < 1:3625227/76‑1 (MQ=255) gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgacg < 2:4604680/76‑1 (MQ=255) cccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCATCTgtcga > 2:6307667/1‑76 (MQ=255) ccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgacgac < 2:3220347/76‑1 (MQ=255) cGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGacgacg > 1:5646261/1‑76 (MQ=255) ggTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGAcgc > 1:4760779/1‑76 (MQ=255) tAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACgcgt > 2:8308517/1‑76 (MQ=255) tAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACgcgt > 1:3936993/1‑76 (MQ=255) aaaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGAcgcggg > 1:5921784/1‑74 (MQ=255) aaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACgcgtgc > 1:1972551/1‑76 (MQ=255) aGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACgcgtgcg < 1:5056477/76‑1 (MQ=255) aGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACgcgtgcg > 2:7130111/1‑76 (MQ=255) cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTc < 2:7619364/76‑1 (MQ=255) cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTc > 1:3111774/1‑76 (MQ=255) aTGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCa > 2:6833194/1‑76 (MQ=255) aTGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGGGCGTTCa > 2:6653883/1‑76 (MQ=255) tGCAGAAACGCTTCGCGCAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGAGTTCAt < 1:6094194/76‑1 (MQ=39) tGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCAt < 1:1758621/76‑1 (MQ=255) gCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATc < 2:6021795/76‑1 (MQ=255) aGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCgg > 1:3736349/1‑76 (MQ=255) gAAACGCTTCGCACAGCAGCTTGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGt < 2:2067287/76‑1 (MQ=255) gAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGt < 2:2169366/76‑1 (MQ=255) aaaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTg < 2:2321409/76‑1 (MQ=255) aaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGa > 2:5831610/1‑76 (MQ=255) aCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAg < 2:1672527/76‑1 (MQ=255) gCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGa < 1:6414480/76‑1 (MQ=255) gCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGa > 2:6577863/1‑76 (MQ=255) cTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAgggt > 1:6725971/1‑74 (MQ=255) tCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAttt < 1:511781/76‑1 (MQ=255) cGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtttt > 1:7759760/1‑76 (MQ=255) cGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtttt < 2:124956/76‑1 (MQ=255) cGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtttt > 1:3529361/1‑76 (MQ=255) gCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAttttt < 1:1161307/76‑1 (MQ=255) gCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAttttt < 2:4935170/76‑1 (MQ=255) cacaGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGGGAGGATTTTTa > 2:6939169/1‑76 (MQ=255) cagcagCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAac < 1:7669841/76‑1 (MQ=255) agcagcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAaca < 1:1673541/76‑1 (MQ=255) cagcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACAcc > 2:2560370/1‑76 (MQ=255) cagcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGGGCGTTCATCGGTGAGGATTTTTAAAAcc > 2:1214858/1‑76 (MQ=255) gcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt < 2:2745798/76‑1 (MQ=255) gcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt < 2:8031523/76‑1 (MQ=255) gcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt < 1:1513652/76‑1 (MQ=255) cTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTa < 2:6287262/76‑1 (MQ=255) tCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTaa < 2:4397747/76‑1 (MQ=255) cGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAag < 1:2380311/76‑1 (MQ=255) cGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAag < 2:5539373/76‑1 (MQ=255) gCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAaga < 1:2695806/76‑1 (MQ=255) tGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAgg > 1:5208764/1‑76 (MQ=255) tGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGGGAGGATTTTTAACACCTTAAgggg > 1:5392781/1‑76 (MQ=255) gTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGa < 2:2671207/76‑1 (MQ=255) ttGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGaa < 2:3705491/76‑1 (MQ=255) ttGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGaa < 1:872449/76‑1 (MQ=255) ttGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGaa < 1:1604379/76‑1 (MQ=255) ttGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGaa < 1:6664178/76‑1 (MQ=255) tGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTCAGAGGAAt > 2:1305558/1‑76 (MQ=255) gTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATgg < 1:2502782/76‑1 (MQ=255) gTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATgg < 1:752011/76‑1 (MQ=255) tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa < 2:3070394/76‑1 (MQ=255) tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa > 1:4941791/1‑76 (MQ=255) tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa < 1:1208950/76‑1 (MQ=255) tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa < 1:8158783/76‑1 (MQ=255) tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa < 2:7824753/76‑1 (MQ=255) tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa < 1:7222621/76‑1 (MQ=255) aGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGaa < 1:2213939/76‑1 (MQ=255) aGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGaa > 1:3809239/1‑76 (MQ=255) aGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGaa > 1:6172538/1‑76 (MQ=255) cGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGGTTTTTAACACCTTAAGAGGAATGGAAAc > 1:5861957/1‑76 (MQ=255) cGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAc > 1:6251508/1‑76 (MQ=255) gTGACGCAGGTTGTTCACCTGACGACGCGTGCGGTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc < 1:3966562/76‑1 (MQ=255) gTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc > 1:1295451/1‑76 (MQ=255) gTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc < 2:3526240/76‑1 (MQ=255) gTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc < 2:3250869/76‑1 (MQ=255) tGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCg > 2:5430398/1‑76 (MQ=255) tGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCg < 1:4644784/76‑1 (MQ=255) gACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGt > 1:996421/1‑76 (MQ=255) gACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGt < 1:4515807/76‑1 (MQ=255) gACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGt > 2:174794/1‑76 (MQ=255) aCGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTa < 2:4729139/76‑1 (MQ=255) cGCAGGCTGTTCACCTGACGACGCGTTCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTaa < 1:8364799/76‑1 (MQ=255) cGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTaa < 2:2956394/76‑1 (MQ=255) cGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTaa < 2:7433413/76‑1 (MQ=255) cGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTaa < 2:6059/76‑1 (MQ=255) gCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAAt > 1:6503787/1‑76 (MQ=255) gCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAAt < 1:695004/76‑1 (MQ=255) gCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAAt < 2:3904172/76‑1 (MQ=255) | CGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCT > REL606/4108287‑4108432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |