| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,201,918 | C→A | E214D (GAG→GAT) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,201,918 | 0 | C | A | 99.7% | 1444.7 / ‑6.1 | 340 | E214D (GAG→GAT) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base A (151/187); minor base G (1/0); total (153/187) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 351 total aligned reads displayed.
AAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGC > REL606/4201845‑4201990 | aaTTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTtcatca > 1:1513041/1‑76 (MQ=255) aaTTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCa > 2:4914476/1‑76 (MQ=255) tGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCg > 1:4884458/1‑76 (MQ=255) gCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGTCCCAGCGCATGTTCGTCATCgc > 1:4164491/1‑75 (MQ=255) gCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGt < 1:3933783/76‑1 (MQ=255) cGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTc < 2:8161235/76‑1 (MQ=255) cGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTc < 1:4682717/76‑1 (MQ=255) cGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTc < 2:1149296/76‑1 (MQ=255) ggCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCa < 2:1492695/76‑1 (MQ=255) ggCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCa < 1:4029704/76‑1 (MQ=255) gCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCaa < 2:3869695/76‑1 (MQ=255) cAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCaaa < 1:3646013/76‑1 (MQ=255) aaaCGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAAt < 1:7968055/76‑1 (MQ=255) aaaCGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAAt > 1:6712739/1‑76 (MQ=255) aaaCGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAAt > 2:2055379/1‑76 (MQ=255) aaaCGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAAt < 1:7902440/76‑1 (MQ=255) cGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGaa < 1:7539580/76‑1 (MQ=255) ggTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAAt < 1:1165307/76‑1 (MQ=255) ggTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAAt < 2:7958087/76‑1 (MQ=255) gTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATa < 1:7088962/76‑1 (MQ=255) gTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATa < 2:1750757/76‑1 (MQ=255) ttCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATaa < 2:544963/76‑1 (MQ=255) cACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAAcc > 1:3483397/1‑76 (MQ=255) cACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAAcc > 1:7729033/1‑76 (MQ=255) cACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGGTCATCATCGTCAAATGAATAAcc > 2:4493368/1‑76 (MQ=255) aCGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAAccc > 2:668368/1‑76 (MQ=255) cGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAAcccc < 2:2256265/76‑1 (MQ=255) gTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGt > 1:3355224/1‑76 (MQ=255) tGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGtt > 2:8266890/1‑76 (MQ=255) gCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGttt < 2:1004860/76‑1 (MQ=255) gCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGttt > 1:6970847/1‑76 (MQ=255) gCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGttt < 1:5520902/76‑1 (MQ=255) gCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGttt > 2:4015532/1‑76 (MQ=255) gCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGttt < 2:5231162/76‑1 (MQ=255) cTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTg < 2:2614092/76‑1 (MQ=255) cTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTg < 1:6665696/76‑1 (MQ=255) tCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTgc < 2:6904098/76‑1 (MQ=255) tCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTgc < 2:1883973/76‑1 (MQ=255) cATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTgcg > 1:5672167/1‑76 (MQ=255) cATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTgcg > 2:5987762/1‑76 (MQ=255) cATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTgcg > 2:6154359/1‑76 (MQ=255) cATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTgcg > 1:3996925/1‑76 (MQ=255) tCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGcgcg > 2:2848903/1‑76 (MQ=255) cGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGt > 1:5215761/1‑76 (MQ=255) cGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGt > 1:4436336/1‑76 (MQ=255) aaaaTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGttt < 2:7510548/76‑1 (MQ=255) aaaaTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGttt < 1:4121168/76‑1 (MQ=255) aaaaTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGttt > 2:7890255/1‑76 (MQ=255) aaTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTgg < 1:6857316/76‑1 (MQ=255) aTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTggg < 1:3726051/76‑1 (MQ=255) aTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTggg > 2:3525969/1‑76 (MQ=255) tACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGc < 2:410063/76‑1 (MQ=255) acacGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCg < 2:2688918/76‑1 (MQ=255) acacGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCg < 2:3143715/76‑1 (MQ=255) acacGCTGCAAGCCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCg < 2:220136/76‑1 (MQ=255) cacGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCga > 2:573498/1‑76 (MQ=255) acGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCgag < 1:2212891/76‑1 (MQ=255) acGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCgag < 2:8355914/76‑1 (MQ=255) cGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCgaga < 1:6173014/76‑1 (MQ=255) gCTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGAt > 2:7675736/1‑76 (MQ=255) cTGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGAtc > 1:1306936/1‑76 (MQ=255) tGCAAGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGAtct > 1:7263317/1‑76 (MQ=255) aaGGCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGAtcttct > 2:1439265/1‑76 (MQ=255) ggCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCttt < 1:1053897/76‑1 (MQ=255) ggCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCttt < 1:8112756/76‑1 (MQ=255) gCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCtttt < 1:2065940/76‑1 (MQ=255) gCAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCtttt < 1:5115385/76‑1 (MQ=255) cAACGTAGCCCCAGCGCATGTTCATCATCGTGAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTa > 2:6449477/1‑76 (MQ=255) cAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTa > 2:1322375/1‑76 (MQ=255) cAACGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTa < 1:2490196/76‑1 (MQ=255) aaCGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTaa < 2:7074689/76‑1 (MQ=255) aaCGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTaa > 1:5538711/1‑76 (MQ=255) aCGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTaac < 1:1657588/76‑2 (MQ=255) aCGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTaaa > 2:4292815/1‑76 (MQ=255) aCGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTaaa < 1:6573976/76‑1 (MQ=255) cGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAAt > 2:3597866/1‑76 (MQ=255) cGTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAAt < 2:1555888/76‑1 (MQ=255) gTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAGATg > 1:8361983/1‑76 (MQ=255) gTAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATg > 1:6492328/1‑76 (MQ=255) tAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGc > 2:3262389/1‑76 (MQ=255) tAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGc < 1:4896769/76‑1 (MQ=255) tAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGc < 2:7005388/76‑1 (MQ=255) tAGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGc > 2:2533839/1‑76 (MQ=255) aGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGca > 1:3297867/1‑76 (MQ=255) aGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGca > 2:7767489/1‑76 (MQ=255) aGCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGca > 2:4176139/1‑76 (MQ=255) gCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGcac < 1:5856882/76‑1 (MQ=255) gCCCCAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGcac < 1:4612045/76‑1 (MQ=255) ccccAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGcaca < 2:5488106/76‑1 (MQ=255) ccccAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGcaca > 2:7159319/1‑76 (MQ=255) ccAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAgg < 2:5806865/76‑1 (MQ=255) ccAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAgg > 1:6123773/1‑76 (MQ=255) ccAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAgg < 2:5281706/76‑1 (MQ=255) ccAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAgg < 2:5014181/76‑1 (MQ=255) ccAGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAgg > 2:4657160/1‑76 (MQ=255) aGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGgag > 2:6013079/1‑76 (MQ=255) aGCGCATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGgag < 2:4332926/76‑1 (MQ=255) cgcATGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGac < 1:4267054/76‑1 (MQ=255) aTGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACAcc > 1:3170734/1‑76 (MQ=255) aTGTTCATCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACAcc < 1:1014598/76‑1 (MQ=255) | AAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGC > REL606/4201845‑4201990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |