| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,027,164 | (A)6→5 | coding (507/660 nt) | ECB_02827 ← | Polysialic acid transport ATP‑binding KpsT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,027,159 | 0 | A | . | 95.9% | 694.2 / 21.5 | 146 | coding (512/660 nt) | ECB_02827 | Polysialic acid transport ATP‑binding KpsT |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base . (81/59); total (83/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 168 total aligned reads displayed.
ACCGCGTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTAAAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGACTG > REL606/3027084‑3027231 | aCCGCGTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTa < 2:3081674/76‑1 (MQ=255)aCCGCGTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTa < 1:852213/76‑1 (MQ=255) ccGCGTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaa < 1:2103063/76‑1 (MQ=255) cgTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaa < 1:5256383/76‑1 (MQ=255) ttATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaact > 1:501685/1‑74 (MQ=255) ttATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaact < 1:5871711/76‑3 (MQ=255) ttATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaact > 1:6972551/1‑74 (MQ=255) ttATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaact > 2:225087/1‑74 (MQ=255) tATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTaaaaacta > 2:1835078/1‑73 (MQ=255) tATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATCCAAACTATGTGAAACCATTaaaaacta > 2:5219596/1‑73 (MQ=255) aTTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGAtt > 1:1752199/1‑76 (MQ=255) aTTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGAtt < 1:661210/76‑1 (MQ=255) aTTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGAtt < 1:3529445/76‑1 (MQ=255) aTTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGAtt < 1:5943175/76‑1 (MQ=255) ttttAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCt < 1:3038305/76‑1 (MQ=255) tttAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCtt < 2:7233639/76‑1 (MQ=255) ttAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCttt > 1:6335743/1‑76 (MQ=255) ttAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCttt > 1:6042181/1‑76 (MQ=255) ttAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCttt > 1:4912005/1‑76 (MQ=255) tAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTa > 1:6832602/1‑76 (MQ=255) aaaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTAt < 2:7089334/76‑1 (MQ=255) aaaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTAt > 2:5435000/1‑76 (MQ=255) aaaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTAt < 1:1134192/76‑1 (MQ=255) aaaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTAt > 1:1402641/1‑76 (MQ=255) aaaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTAt > 1:2179664/1‑76 (MQ=255) aaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATg > 2:4241686/1‑76 (MQ=255) aaTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATg > 2:4397120/1‑76 (MQ=255) aTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGc > 2:2700937/1‑76 (MQ=255) aTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGc > 2:6447183/1‑76 (MQ=255) aTACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGc > 1:7506529/1‑76 (MQ=255) aCAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCct < 1:6922577/76‑1 (MQ=255) aCAAAGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCct > 1:5079954/1‑76 (MQ=255) cAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctt < 2:6987992/76‑1 (MQ=255) cAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctt > 1:1586668/1‑76 (MQ=255) cAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctt > 1:3475078/1‑76 (MQ=255) cAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctt > 1:329117/1‑76 (MQ=255) ggCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttctt > 1:2266732/1‑76 (MQ=255) ggCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttctt > 1:4483795/1‑76 (MQ=255) gCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttcttt < 1:3116442/76‑1 (MQ=255) gCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttcttt < 2:744120/76‑1 (MQ=255) gCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttcttt < 2:4849979/76‑1 (MQ=255) gCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCctttcttt < 2:2333286/76‑1 (MQ=255) cacaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaa > 2:5913769/1‑76 (MQ=255) cacaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaa < 1:2832092/76‑1 (MQ=255) cacaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaa > 2:1241057/1‑76 (MQ=255) cacaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaa < 2:4793364/76‑1 (MQ=255) acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa > 1:6694491/1‑76 (MQ=255) acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa < 2:3213671/76‑1 (MQ=255) acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa < 2:2665324/76‑1 (MQ=255) acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa < 2:4406166/76‑1 (MQ=255) acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa < 1:5932742/76‑1 (MQ=255) acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa < 2:4529008/76‑1 (MQ=255) acaTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTaaa > 1:7395617/1‑76 (MQ=255) caTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAAc < 2:5083541/76‑1 (MQ=255) caTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAAc > 2:6627761/1‑76 (MQ=255) aTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACa > 2:2090003/1‑76 (MQ=255) aTCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACa < 2:5111184/76‑1 (MQ=255) cGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAAt > 2:5350775/1‑76 (MQ=255) cGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAAt > 1:3225419/1‑76 (MQ=255) gCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAAtt < 1:2062286/76‑1 (MQ=255) gCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAAtt > 1:97422/1‑76 (MQ=255) cAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTg < 2:50214/76‑1 (MQ=255) aaaCTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAg < 1:4020977/76‑1 (MQ=255) aaaCTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAg > 2:1293403/1‑76 (MQ=255) aCTCTTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAgcg > 1:5013188/1‑76 (MQ=255) tctTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCa > 2:6594402/1‑76 (MQ=255) tctTTCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCa > 1:95118/1‑76 (MQ=255) tttCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCAtt > 2:3527318/1‑76 (MQ=255) tCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCAtttt < 2:6257056/76‑1 (MQ=255) tCAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCAtttt < 1:6033117/76‑1 (MQ=255) cAATGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCAttttt < 2:4640446/76‑1 (MQ=255) aaTGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTc > 2:6237492/1‑76 (MQ=255) aaTGAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTc > 2:1659653/1‑76 (MQ=255) gAATTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCttt > 2:855305/1‑76 (MQ=255) aaTTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTa < 1:630819/76‑1 (MQ=255) aaTTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTa < 2:1501532/76‑1 (MQ=255) aTTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTaa > 1:4151539/1‑76 (MQ=255) aTTCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTaa > 1:5743788/1‑76 (MQ=255) ttCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTaaa > 1:5524204/1‑76 (MQ=255) tCAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAAc > 2:3041722/1‑76 (MQ=255) cAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAAcc > 1:67905/1‑76 (MQ=255) cAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAAcc < 2:5789676/76‑1 (MQ=255) cAAACTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAAcc > 2:5298015/1‑76 (MQ=255) aaaCTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCt > 2:3857681/1‑76 (MQ=255) aaaCTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCt > 2:2554798/1‑76 (MQ=255) aaCTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTg > 1:6203290/1‑76 (MQ=255) aaCTATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTg < 2:2847412/76‑1 (MQ=255) tATGTGAAACCATTAAAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGc > 2:5478355/1‑76 (MQ=255) tATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCa > 2:2790273/1‑76 (MQ=255) tATGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCa > 1:6450079/1‑76 (MQ=255) tATGTGAAACCATT‑AAAAACTAGATTCTTTAGGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCa > 2:5805140/1‑76 (MQ=255) aTGTGAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCAt < 1:1250876/76‑1 (MQ=255) gtgAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCa > 2:4017279/1‑76 (MQ=255) gAAACCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCAcc > 2:5508415/1‑76 (MQ=255) aaaCCATTAAAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCAcc < 2:1716487/76‑1 (MQ=255) aaCCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGa < 2:1499975/76‑1 (MQ=255) aCCATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGAc < 1:2843386/76‑1 (MQ=255) ccATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGACt < 2:5566628/76‑1 (MQ=255) ccATT‑AAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGACt < 1:5347097/76‑1 (MQ=255) aTTAAAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGACTg > 2:3437921/1‑76 (MQ=255) | ACCGCGTTATCATTTTTAAATACAATGGCCACATCGCAAAACTCTTTCAATGAATTCAAACTATGTGAAACCATTAAAAAACTAGATTCTTTATGCCTTTCTTTAAACAATTGAGCGCATTTTTCTTTAAACCTGGCATCACCGACTG > REL606/3027084‑3027231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |