| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,489,365 | Δ1 bp | coding (689/831 nt) | glpG ← | predicted intramembrane serine protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,489,364 | 0 | A | . | 99.0% | 982.5 / 2.5 | 194 | coding (690/831 nt) | glpG | predicted intramembrane serine protease |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (85/107); total (86/108) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 207 total aligned reads displayed.
GATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAG > REL606/3489289‑3489435 | taTGGGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATa < 1:2047823/75‑1 (MQ=255)gATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATa < 2:3611801/76‑1 (MQ=255) aTGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATat < 1:5429067/76‑2 (MQ=255) aTGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATat > 1:3097833/1‑75 (MQ=255) tgtgtgCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATatt > 1:1631605/1‑74 (MQ=255) gtgtcCCCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATatta < 2:5706688/76‑4 (MQ=255) gtgCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAAc > 1:4233825/1‑76 (MQ=255) gtgCTCCGTTCCCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAAc < 1:6241301/76‑1 (MQ=255) gtccTCCTTCCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAAc < 2:4042568/73‑1 (MQ=255) tgCTCCGTCCCCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAAcc < 1:6325585/76‑1 (MQ=255) tgCCCCGTTCCCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAAcc < 1:4880240/76‑1 (MQ=255) gCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAAccc > 1:1437882/1‑76 (MQ=255) cTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCa < 2:3027453/76‑1 (MQ=255) cTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCa < 2:1504103/76‑1 (MQ=255) cTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCa < 2:7425723/76‑1 (MQ=255) cTCCGTCCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCa < 2:5327495/76‑1 (MQ=255) tCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCAc > 1:2470653/1‑76 (MQ=255) ccGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACg > 2:6385311/1‑76 (MQ=255) ccGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACg > 1:5606285/1‑76 (MQ=255) ttCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTg > 1:3119386/1‑76 (MQ=255) ttCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTg < 2:236679/76‑1 (MQ=255) ttCCCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTg < 2:4926203/76‑1 (MQ=255) cGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCa < 2:651247/76‑1 (MQ=255) cATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGt < 1:4344135/76‑1 (MQ=255) aTCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTa < 2:902778/76‑1 (MQ=255) cGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTaaa > 1:6037323/1‑76 (MQ=255) gACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAAt < 2:5415512/76‑1 (MQ=255) aCATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATg > 2:6720027/1‑76 (MQ=255) aCATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATg > 1:1996516/1‑76 (MQ=255) aTCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGcc > 2:5457772/1‑76 (MQ=255) tCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCa > 2:2700505/1‑76 (MQ=255) tCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCa > 2:7125318/1‑76 (MQ=255) ccAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACt < 2:5245831/76‑1 (MQ=255) aaacaaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACttt > 2:4349058/1‑76 (MQ=255) aaacaaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACttt < 1:6996883/76‑1 (MQ=255) acaaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGc < 1:2260696/76‑1 (MQ=255) acaaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGc < 1:7313488/76‑1 (MQ=255) caaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCg > 2:7352227/1‑76 (MQ=255) caaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCg > 2:5924854/1‑76 (MQ=255) aaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTACAGGTAAATGCCACTTTGCgg > 2:209912/1‑76 (MQ=255) aaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGa < 2:331001/76‑1 (MQ=255) aTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGAt < 2:7211038/76‑1 (MQ=255) tCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATc < 2:7254395/76‑1 (MQ=255) cAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATcg > 1:6857587/1‑76 (MQ=255) aaaCCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATcgc > 2:2514680/1‑76 (MQ=255) aaaCCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATcgc < 2:5081854/76‑1 (MQ=255) aaaCCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATcgc > 2:3907835/1‑76 (MQ=255) aaaCCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATcgc > 1:3218089/1‑76 (MQ=255) aaaCCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATcgc > 2:4173650/1‑76 (MQ=255) aaaCCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATcgc > 2:5940698/1‑76 (MQ=255) aaaCCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATcgc > 1:4450798/1‑76 (MQ=255) aaCCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATcgcg > 1:1167375/1‑76 (MQ=255) aCCATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGt < 2:3387404/76‑1 (MQ=255) cATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTc < 2:1832765/76‑1 (MQ=255) cATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTc < 2:1436655/76‑1 (MQ=255) cATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTc > 2:4556248/1‑76 (MQ=255) cATCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTc < 2:3515267/76‑1 (MQ=255) cATCAGGCGACAATCCAGATCAGCGCAAGGAT‑ATTAACCCACGTTGCAGGTAAATTCCACTATGCGTATCAGGTTc > 2:31900/1‑76 (MQ=255) aTCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCg > 2:6954562/1‑76 (MQ=255) aTCCGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCg > 1:5811513/1‑76 (MQ=255) cGGCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCa > 2:262260/1‑76 (MQ=255) ggCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCAc > 1:2736985/1‑76 (MQ=255) gCGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACg < 1:2032225/76‑1 (MQ=255) cGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGt > 2:3704179/1‑76 (MQ=255) cGACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGAATCGCGTTCGCCACgc > 2:7034767/1‑75 (MQ=255) gACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTa < 1:2109813/76‑1 (MQ=255) gACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTa < 1:4130926/76‑1 (MQ=255) gACAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTa < 1:4040707/76‑1 (MQ=255) aCAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAg > 1:6659731/1‑76 (MQ=255) aCAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAg > 1:3817389/1‑76 (MQ=255) cAATCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGc > 2:415601/1‑76 (MQ=255) aaTCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGcc < 2:429744/76‑1 (MQ=255) aTCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCa < 1:314610/76‑1 (MQ=255) aTCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCa > 1:5374510/1‑76 (MQ=255) aTCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCa < 2:2568151/76‑1 (MQ=255) tCCAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAg > 1:5913080/1‑76 (MQ=255) cAGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGAc > 1:430698/1‑76 (MQ=255) aGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACg < 2:7398867/76‑1 (MQ=255) aGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACg > 2:5521130/1‑76 (MQ=255) aGATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACg > 2:5782557/1‑76 (MQ=255) gATCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGt > 2:6060393/1‑76 (MQ=255) aTCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTa > 1:7096846/1‑76 (MQ=255) aTCAGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGCAGCCACACgca > 1:50884/1‑74 (MQ=255) acAGCGCAAAGAT‑ATTAACCCCGTTTGCAGGTAAATGCCACTTTGCGGATCGCGTGCGCCCCGTAGCCAGACGTAg < 2:3122416/75‑1 (MQ=255) cAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGCAg > 2:3937391/1‑76 (MQ=255) aGCGCAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGcc > 2:6814396/1‑76 (MQ=255) gcgcAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGccc > 2:6144396/1‑76 (MQ=255) gcgcAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGccc > 1:7395636/1‑76 (MQ=255) gcgcAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTGGCGGATCGCGTTCGCCACGTAGCCAGACGTAGccc < 1:6456201/76‑1 (MQ=255) cgcAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCa < 2:6638729/76‑1 (MQ=255) cgcAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCa > 2:1118365/1‑76 (MQ=255) gcAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCAt < 1:4813933/76‑1 (MQ=255) gcAAAGAT‑ATTAACCCACGTTGCAGGTAAAGGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCAt < 1:1923683/76‑1 (MQ=255) cAAAGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATc > 2:158774/1‑76 (MQ=255) aaaGAT‑ATTACCCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCa < 2:4730544/76‑1 (MQ=255) aaaGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCa < 2:3622077/76‑1 (MQ=255) aaaGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCa < 1:4994925/76‑1 (MQ=255) aaaGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCa < 2:5233436/76‑1 (MQ=255) aaGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAg > 1:674372/1‑76 (MQ=255) aaGAT‑ATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAg < 2:2209409/76‑1 (MQ=255) | GATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAG > REL606/3489289‑3489435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |