Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 3,911,972 (T)7→8 intergenic (‑173/‑150) yifB ← / → ilvL predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,911,9651.T100.0% 786.1 / NA 184intergenic (‑166/‑157)yifB/ilvLpredicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (62/122);  total (62/122)

Only 100 of 208 total aligned reads displayed.

GAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAA  >  REL606/3911891‑3912035
                                                                           |                                                                      
gAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑t                                                                       <  2:2791018/76‑1 (MQ=255)
  atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt                                                                     >  1:148318/1‑76 (MQ=255)
  atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt                                                                     <  2:6635258/76‑1 (MQ=255)
  atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt                                                                     <  2:6532080/76‑1 (MQ=255)
  atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt                                                                     <  1:5477550/76‑1 (MQ=255)
  atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt                                                                     <  1:56683/76‑1 (MQ=255)
  atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt                                                                     >  1:2469809/1‑76 (MQ=255)
     atgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttt                                                                  >  1:4553515/1‑76 (MQ=255)
     atgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttt                                                                  >  2:6653917/1‑76 (MQ=255)
     atgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttt                                                                  <  1:7489960/76‑1 (MQ=255)
      tgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttt                                                                 <  2:5268627/76‑1 (MQ=255)
         tCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttttca                                                              <  1:6969149/76‑4 (MQ=255)
            cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactc                                                            <  2:7046526/76‑1 (MQ=255)
            cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactc                                                            <  1:864895/76‑1 (MQ=255)
             aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactca                                                           <  2:341134/76‑1 (MQ=255)
             aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactca                                                           <  1:6818034/76‑1 (MQ=255)
              aaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactcac                                                          >  2:3981873/1‑76 (MQ=255)
               aaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTcactcact                                                         <  2:1381364/76‑1 (MQ=255)
                aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta                                                        <  2:272000/76‑1 (MQ=255)
                aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta                                                        >  1:3222943/1‑76 (MQ=255)
                aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta                                                        <  2:372694/76‑1 (MQ=255)
                aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta                                                        <  1:2045033/76‑1 (MQ=255)
                 aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtat                                                       <  1:4144275/76‑1 (MQ=255)
                  tGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatt                                                      <  2:1289129/76‑1 (MQ=255)
                   gCAGCGGACAAAGGATGAACTACGAGGAATGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt                                                     <  2:4816565/76‑1 (MQ=255)
                   gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt                                                     <  2:3254437/76‑1 (MQ=255)
                   gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt                                                     <  2:1083407/76‑1 (MQ=255)
                   gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt                                                     >  1:6375141/1‑76 (MQ=255)
                   gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt                                                     <  2:7321754/76‑1 (MQ=255)
                    cAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttt                                                    >  1:2492765/1‑76 (MQ=255)
                    cAGCGGACAAAGGAGGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttt                                                    <  1:3411054/76‑1 (MQ=255)
                          aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTAttttt                                              <  1:5505855/76‑1 (MQ=255)
                           cAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTa                                             <  1:4812970/76‑1 (MQ=255)
                           cAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTa                                             <  1:29632/76‑1 (MQ=255)
                            aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaa                                            >  2:1815835/1‑76 (MQ=255)
                            aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaa                                            <  2:528509/76‑1 (MQ=255)
                            aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaa                                            >  1:5720847/1‑76 (MQ=255)
                            aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaa                                            <  2:3501339/76‑1 (MQ=255)
                              aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaa                                          >  1:3898633/1‑76 (MQ=255)
                               ggATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaaa                                         <  2:1524355/76‑1 (MQ=255)
                                gATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaaaa                                        <  1:7330940/76‑1 (MQ=255)
                                gATGAACTACGAGGAAGAGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaaaa                                        <  2:7222260/76‑1 (MQ=255)
                                  tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaaca                                      <  1:3383597/76‑1 (MQ=255)
                                    aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaac                                    >  1:3583004/1‑76 (MQ=255)
                                    aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaac                                    <  2:5091707/76‑1 (MQ=255)
                                    aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaac                                    <  1:6581461/76‑1 (MQ=255)
                                    aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaac                                    >  1:1101084/1‑76 (MQ=255)
                                     aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAacaaca                                   <  2:2389997/76‑1 (MQ=255)
                                     aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAacaaca                                   <  1:2222678/76‑1 (MQ=255)
                                     aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAacaaca                                   <  1:1414307/76‑1 (MQ=255)
                                        aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAtt                                >  2:2490037/1‑76 (MQ=255)
                                        aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAtt                                <  1:42432/76‑1 (MQ=255)
                                        aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAtt                                >  1:2550599/1‑76 (MQ=255)
                                        aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAtt                                >  2:120756/1‑76 (MQ=255)
                                         cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAttt                               <  1:2354001/76‑1 (MQ=255)
                                         cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAttt                               <  2:6164559/76‑1 (MQ=255)
                                          gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTta                              >  1:6848584/1‑76 (MQ=255)
                                           aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat                             <  1:6021209/76‑1 (MQ=255)
                                           aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat                             <  1:5992549/76‑1 (MQ=255)
                                           aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat                             <  1:4741332/76‑1 (MQ=255)
                                             gAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTatat                           <  2:3113921/76‑1 (MQ=255)
                                              aaGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATAtt                          <  1:5549440/76‑1 (MQ=255)
                                               aGGGAACGACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                         >  1:1689212/1‑76 (MQ=255)
                                               aGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                         <  2:2895854/76‑1 (MQ=255)
                                               aGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                         <  1:1613799/76‑1 (MQ=255)
                                               aGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                         <  2:1876964/76‑1 (MQ=255)
                                               aGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                         <  1:4235513/76‑1 (MQ=255)
                                               aGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                         >  1:5925635/1‑76 (MQ=255)
                                                gggAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGa                        <  1:2821906/76‑1 (MQ=255)
                                                 ggAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa                       <  1:6143869/76‑1 (MQ=255)
                                                 ggAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa                       <  1:3445818/76‑1 (MQ=255)
                                                  gAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaaa                      <  2:7112099/76‑1 (MQ=255)
                                                  gAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaaa                      >  1:1477954/1‑76 (MQ=255)
                                                   aacaacATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAat                     <  1:3721149/76‑1 (MQ=255)
                                                   aacaacATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAat                     <  1:5003429/76‑1 (MQ=255)
                                                   aacaacATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAat                     <  1:4050889/76‑1 (MQ=255)
                                                    acaacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatt                    >  2:4314224/1‑76 (MQ=255)
                                                    acaacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatt                    <  1:3137658/76‑1 (MQ=255)
                                                     caacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatta                   >  1:234910/1‑76 (MQ=255)
                                                      aacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattat                  >  2:6451711/1‑76 (MQ=255)
                                                        caTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta                <  1:522158/76‑1 (MQ=255)
                                                        caTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta                <  2:5591/76‑1 (MQ=255)
                                                         aTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaa               <  1:2858559/76‑1 (MQ=255)
                                                           tCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAAc             <  2:5728171/76‑1 (MQ=255)
                                                            cATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg            <  2:2142301/76‑1 (MQ=255)
                                                            cATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg            >  1:7114/1‑76 (MQ=255)
                                                            cATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg            >  2:6615185/1‑76 (MQ=255)
                                                            cATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg            >  2:6263831/1‑76 (MQ=255)
                                                            cATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg            >  1:4963978/1‑76 (MQ=255)
                                                               aCTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat         <  2:5392299/76‑1 (MQ=255)
                                                               aCTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat         >  2:1637907/1‑76 (MQ=255)
                                                                  gAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat      <  1:6222294/76‑1 (MQ=255)
                                                                  gAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat      >  1:3725962/1‑76 (MQ=255)
                                                                   aaaTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa     >  2:5967471/1‑76 (MQ=255)
                                                                   aaaTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa     <  2:507332/76‑1 (MQ=255)
                                                                    aaTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa    <  1:1552347/76‑1 (MQ=255)
                                                                    aaTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa    <  2:4167593/76‑1 (MQ=255)
                                                                     aTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa   <  2:6712158/76‑1 (MQ=255)
                                                                      ttGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa  >  1:3271407/1‑76 (MQ=255)
                                                                      ttGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa  >  1:2035548/1‑76 (MQ=255)
                                                                           |                                                                      
GAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAA  >  REL606/3911891‑3912035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑