| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,854,401 | 0 | A | C | 50.6% | 60.2 / 110.8 | 81 | K88N (AAA→AAC) | yeaO | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (12/28); new base C (0/41); total (12/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.90e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-07 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 115 total aligned reads displayed.
CTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTA > REL606/1854326‑1854473 | cTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGGGGGG‑AGAGCCaaaaaa > 1:1840138/1‑76 (MQ=255)cTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGGGGGC‑AACGCCcaaaaa > 2:3471090/1‑76 (MQ=255) tttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGGA‑AACGTCCAAAAAc > 2:605080/1‑76 (MQ=255) ttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGGGGGCAATCCCCaaaaaaa > 1:1904765/1‑75 (MQ=255) ttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGGGGGC‑AACGCCcacaaaaa > 1:6365080/1‑74 (MQ=255) ttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACa < 1:2316265/76‑1 (MQ=255) tCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAg < 2:7340922/76‑1 (MQ=255) tCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAg < 2:1069371/76‑1 (MQ=255) tCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAg < 1:4100697/76‑1 (MQ=255) tCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAg < 1:2077896/76‑1 (MQ=255) tCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAg < 1:3627623/76‑1 (MQ=255) gCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTg < 1:1437770/76‑1 (MQ=255) gCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCAAAAAACCAGCCGCTg < 2:3453779/76‑1 (MQ=255) gCAATATCTTGCAGAACTGGCGCAACACGAGAAAGAAGGAAAGCGGCG‑GGCGGAC‑ATCGCAAAAAACCAGCCGCTg < 2:2206508/76‑1 (MQ=255) cAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGGGGGC‑AACGCCCAAAAAAAACCGccgc > 2:7203079/1‑73 (MQ=255) aaTATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGGGGGG‑ATAGCCCAACAAAAACCGCTGaa > 1:2110952/1‑75 (MQ=255) atCTTGCAGACCGGGCGCAACACGAGAAAGAAGGAAGGCGGGT‑GGCGGAC‑ATCCCAAAAAACCAGCCGCTGACCCt < 2:3951984/76‑1 (MQ=255) atCTGGCAGAGCGGGCACAACCCGAGCAAAAAGGAAACCGGGT‑GGCAGAC‑ACCCCCAAAAACCAGCCGCTGACCCt < 2:4424317/76‑1 (MQ=255) tGCAGAACGGGCGCAACACGACCAAGAGGGAAAGCGGCT‑GGCGGAC‑ATCGCC‑AAAACCAGCCGCTGACCCTGctct < 2:7334416/76‑1 (MQ=255) gCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGctct < 1:2588418/76‑1 (MQ=255) gCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGctct < 1:1131008/76‑1 (MQ=255) gCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCG‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGctct < 2:698358/76‑1 (MQ=255) gCAGAACTGGCGCAACACGAGCAAGAAGAAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGctct < 1:1740810/76‑1 (MQ=255) cAGAACTGGCGCAACGCGAGCAAAAAGAAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTa < 1:652462/76‑1 (MQ=255) cAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTa < 1:4068113/76‑1 (MQ=255) cAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTa < 1:5484783/76‑1 (MQ=255) aaaaCGGGCGAAACGCGAGAAAGAAGGAAAGCGGCG‑GGCGGAC‑TTCGCCAAAAACCAGCCGCTGACCCTGCTCTAc < 2:2938619/74‑1 (MQ=255) aGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTAc < 1:7467020/76‑1 (MQ=255) aGAACTGGCGCAACACGAGAAAGAAGGAAGGCGGCG‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTAc < 2:1622684/76‑1 (MQ=255) aCTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACgca < 1:5305759/76‑1 (MQ=255) cgggCGCAACACGAGCAAGAAGAAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACgcag < 2:4130920/74‑1 (MQ=255) cTGGCCAAACACGAGAAAGAGGGAAAGCGGCG‑GGCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACgcag < 2:4699950/76‑1 (MQ=255) tGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACgcagc < 1:6642700/76‑1 (MQ=255) gggCGCAACACGAGCAAGAAGGAAAGGGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACgcagc < 1:1433391/75‑1 (MQ=255) gggCGCAACACGAGAAAGAAGGAAAGCGGCG‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACgcagc < 2:636810/75‑1 (MQ=255) gggCGAAACACGAGCAAGAAGGAAAGCGGCG‑GGCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACgcagc < 2:6577214/75‑1 (MQ=255) ggCGCACCACGAGCAAGAAGAAAAGCGGCT‑GGCGGAC‑AGCGCCAAAAACCAGCCGCTGACCCTGCTCTACgcagca < 2:6451900/76‑1 (MQ=255) ggCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACgcagca < 1:2460508/76‑1 (MQ=255) ggCGCAACACGAGAAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACgcagca < 2:3809587/76‑1 (MQ=255) cAACACGAGAAAGAAGGAAGGCGGCT‑GGCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaaaa < 2:5274366/76‑1 (MQ=255) aaCACAAGCAAGAAGAAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaaaaaa < 1:81894/76‑1 (MQ=255) cacGAGAAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAaca < 2:4422626/76‑1 (MQ=255) aaggagaAAGAAGGAAAGCGGCT‑GGCGTAC‑TTCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAaca < 1:3479779/73‑1 (MQ=255) acGAGCAAGAAGGAAAGCGGCT‑GGGGGGG‑ATCGCCCAAAAAAAAACGCCGGCCCTGCCCTCTCCAGCcaaaaaaaa > 2:5962254/1‑73 (MQ=255) gAGCAAGAAGGAAAGCGGCTGGGGGGTT‑TTCTCCAAAAAAAACCCCCGGACCCGCTTCCCCGCACCAAAAAAaacc > 2:5262654/1‑76 (MQ=255) aGCAAGAGGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcaccac < 1:2973865/76‑1 (MQ=255) aaggaagGAAAGGGGCT‑GGCGGCC‑ATCGCAAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGc < 1:7080202/73‑1 (MQ=255) agaagaAAAGCGGCG‑GGCGAAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAg < 2:5741600/76‑1 (MQ=255) agaagGAAAGCGGCG‑GGCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAg < 2:7020283/76‑1 (MQ=255) aaaagGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAg < 2:2757288/74‑1 (MQ=255) aagGAAAGGGGCT‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGaa < 1:2836197/76‑1 (MQ=255) aagGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGaa < 1:1444594/76‑1 (MQ=255) agGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAc < 2:1934417/76‑1 (MQ=255) ggAAAGCGGCT‑GGCGGAC‑ATCGCAAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAcc < 1:2754559/76‑1 (MQ=255) ggAAAGCGGCG‑GGGGGAC‑ACCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAcc < 2:1970400/76‑1 (MQ=255) gAAGGCGGCG‑GGCCGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCa < 1:6935915/76‑1 (MQ=255) aaaGCGGCG‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCAt < 2:241698/76‑1 (MQ=255) aggCGGCT‑GGCGGAA‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATg < 2:5143063/74‑1 (MQ=255) aaGCGGCT‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATg < 1:1550402/76‑1 (MQ=255) aGCGGTT‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgc < 2:6625457/76‑1 (MQ=255) aGCGGCT‑GGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgc < 1:1757370/76‑1 (MQ=255) aGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgc < 1:5379438/76‑1 (MQ=255) aGCGGCG‑GGCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgc < 2:7023101/76‑1 (MQ=255) gggCT‑GGCGGAC‑AGCGCCAAAAACCAGCCGCTGTCCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgcgc < 1:3690686/75‑1 (MQ=255) gCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCta > 2:3865310/1‑75 (MQ=255) cT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg > 2:281789/1‑76 (MQ=255) cT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAACATGCGCTgg > 2:4994288/1‑76 (MQ=255) t‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACAACGCAGAACCATGCGCTGGt > 2:6807884/1‑76 (MQ=255) t‑GGCGGAC‑ACCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt < 1:1357182/76‑1 (MQ=255) cgggCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg < 2:6903156/74‑1 (MQ=255) gggCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt < 2:1302077/75‑1 (MQ=255) gggCGGAC‑ACCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt < 1:2788276/75‑1 (MQ=255) gggCGAAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt < 1:393505/75‑1 (MQ=255) gcgcAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGc < 2:4420104/76‑1 (MQ=255) cgcaC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt < 2:404699/73‑1 (MQ=255) cGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt > 1:2671861/1‑76 (MQ=255) ggAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAACACACCACGCAGAACCATGCGCTGGTGCTg > 2:3993064/1‑76 (MQ=255) ggAC‑ATCCCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTg < 2:5596573/76‑1 (MQ=255) aC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGc > 2:3387624/1‑76 (MQ=255) aC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGc < 2:4819774/76‑1 (MQ=255) c‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGcc > 2:3582535/1‑76 (MQ=255) caccGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGcc < 2:689944/73‑1 (MQ=255) aTCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg > 2:1948147/1‑76 (MQ=255) aTCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg > 1:1028633/1‑76 (MQ=255) ccccAAAAACCAGCCGCTGCCCCTTCTCTACGCAGCAAAAACCACCACGCAGAACCATGCGCTGGTGCTGGCCGAc < 1:577398/74‑1 (MQ=255) gCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACt > 2:1578696/1‑76 (MQ=255) ccAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg < 1:3840661/76‑1 (MQ=255) ccAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg > 2:4376376/1‑76 (MQ=255) aaaaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGc > 2:4516298/1‑76 (MQ=255) aaaaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGc < 1:508085/76‑1 (MQ=255) aaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 2:5414604/76‑1 (MQ=255) aaaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt > 2:1019923/1‑76 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCCGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 2:5798657/76‑1 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 1:400389/76‑1 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 2:6658218/76‑1 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 2:3857354/76‑1 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 2:329109/76‑1 (MQ=255) aaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa > 2:5540138/1‑76 (MQ=255) aaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa > 2:6799147/1‑76 (MQ=255) aaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa > 1:6190319/1‑76 (MQ=255) | CTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTA > REL606/1854326‑1854473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |