| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,376,348 | 0 | T | G | 40.6% | 228.4 / 51.4 | 155 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| Reads supporting (aligned to +/- strand): ref base T (25/67); new base G (63/0); total (88/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.18e-23 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 205 total aligned reads displayed.
ACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGA > REL606/2376275‑2376422 | aCCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggggg > 2:2676979/1‑76 (MQ=255) ccAGATGAGGCGACTTGTAGACGCCCCCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtggt < 2:7088180/76‑1 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAgggggg > 2:5892861/1‑75 (MQ=255) cAGATGAGTCGAACTGTAGACGCCCCCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGgtggtg < 2:6357866/76‑1 (MQ=255) cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGgtggtg > 1:3174486/1‑76 (MQ=255) cAGATGAGGCGAACGGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGgtggtg < 2:1484960/76‑1 (MQ=255) cAGATGAGGCAAATTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGgtggtg < 2:3960143/76‑1 (MQ=255) aGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGc > 2:5863929/1‑76 (MQ=255) tGAGGCGAACTGTAGACGCCATCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCtt < 1:2077281/76‑1 (MQ=255) ggAGGCGATCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCtt < 2:5394403/75‑1 (MQ=255) gAGGCGAACTGTAGCCGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 1:166185/76‑1 (MQ=255) gAGGCAAACTGTAGACGCCCCCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 2:1416787/76‑1 (MQ=255) aGGCGAACTGTAGACGCCCCCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTg < 2:1315980/76‑1 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTg < 1:7110345/76‑1 (MQ=255) gAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCAt > 1:1169625/1‑76 (MQ=255) gAACTGTAGACCCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCAt < 2:2639310/76‑1 (MQ=255) cTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCCTTGGCATTCg > 1:6688466/1‑76 (MQ=255) cTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGTCATTcc > 1:5276360/1‑75 (MQ=255) cTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTGCCCTTCg > 2:1992931/1‑76 (MQ=255) cTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTGCCATTCg > 1:5194657/1‑76 (MQ=255) cTGTAGACCCCCCTGTTGGCCCTTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCg < 2:3338863/76‑1 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGt > 1:3455455/1‑76 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGGCTTTGCCAtttct > 1:1956716/1‑73 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGTCATTCGTa > 2:3880149/1‑76 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAc > 1:1845818/1‑76 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCATAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGGCATTCGTaac > 1:3972740/1‑74 (MQ=255) gACCCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAccc < 2:279141/76‑1 (MQ=255) cgcGCCCCTTTGGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAccc < 2:5007976/74‑1 (MQ=255) gCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCCCa > 2:6594073/1‑76 (MQ=255) cccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCaa > 1:1390497/1‑76 (MQ=255) cccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGTCATTCGTACCCGCaa > 1:1058235/1‑76 (MQ=255) ccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGCCATTCGTACCCGCAAc > 1:876575/1‑76 (MQ=255) ccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTTCCATTCGTACCCCCaaa > 1:4820356/1‑75 (MQ=255) ccACTTGGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAAc < 2:1733569/76‑1 (MQ=255) cACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACg < 1:7407889/76‑1 (MQ=255) cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCCCAACg > 2:825091/1‑76 (MQ=255) aCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACgg > 2:2094475/1‑76 (MQ=255) aCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCCCAAccg > 2:6818852/1‑74 (MQ=255) cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGGCTTTTCCAATCGTGCCCCCAACAGt > 2:6472963/1‑76 (MQ=255) cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCCACggg > 1:5865066/1‑75 (MQ=255) tttGTCCCCTCCCGCCATCAGAATCGCCCCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTa < 2:528199/76‑1 (MQ=255) tttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTGGCCTTTGTTACCGCCACggga > 1:1105869/1‑74 (MQ=255) ttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAAAGGTaa > 1:6497502/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGCaaa > 1:2837716/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGCCATTCGTACCCCCAACCGGaaa > 1:4137249/1‑76 (MQ=255) gtccccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAac < 1:5404871/73‑1 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGGCTTTGCCATTCGTAACCGCAACGGGAAac > 1:4393589/1‑76 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTTCCATTCGTACCCCCCAAGGTAAac > 1:272032/1‑76 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAac > 1:5042477/1‑76 (MQ=255) tACCCTGCCGCCATCATAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTGCCATTCGTAACCCCCACGGGAAaca > 1:1270530/1‑76 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGTCATTCGTACCCGCAACGGGAAaca > 2:3015766/1‑76 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCCACGGTAAaca > 2:1345757/1‑76 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCCACGGCAAaca > 2:4281014/1‑76 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCCACGGTaaaaa > 2:2511086/1‑74 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGCCTTTGCCATTCGTACCCGCAACGGTAAacac > 2:4815723/1‑76 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAacac > 2:287089/1‑76 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAaaac > 1:6510597/1‑76 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGAGGCTTTGCCATTCGTAGCTGCCAAGGTGAacac > 1:2443936/1‑76 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaa > 2:7257752/1‑76 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTAACCGCAACGGTAAACACaa > 2:2631397/1‑76 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCACTCGTACCCGCAACGGGAAACACaa > 1:1442352/1‑76 (MQ=255) cgcccgccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaa < 1:3757510/73‑1 (MQ=255) cTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaa > 1:3524887/1‑76 (MQ=255) tGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 1:1598045/76‑1 (MQ=255) cgccgccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 2:3809119/75‑1 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaaag > 2:774931/1‑74 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTGCCAATCGTACCCCCCAAGGTAAAAACCAATg > 1:2930249/1‑76 (MQ=255) cgccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGgc < 1:750257/76‑1 (MQ=255) cgccATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGTCATTTGTACCCGCCACGGTTAATACAAAAGgc > 1:3879440/1‑76 (MQ=255) cATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCt > 2:2249856/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACACATGGCGCTg > 1:7325254/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTg > 1:3167385/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCCACGGTAAACACAAATGGCGCTg > 1:7328868/1‑76 (MQ=255) cAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACCAATGGCGCTGGt > 2:741598/1‑76 (MQ=255) aGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCCTTTGTACCCGCAACGGTAAACACAAATGGCGCTgggt > 2:5233967/1‑74 (MQ=255) gAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt < 1:2193830/76‑1 (MQ=255) gAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt > 1:6833170/1‑76 (MQ=255) caaTCGCCTCAAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt < 2:2544938/75‑1 (MQ=255) tCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAg < 1:1742196/76‑1 (MQ=255) tCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAg > 1:1562697/1‑76 (MQ=255) cGACTCCAGCGTACGGCAGGGGGTGCCTTTGGCATTCGTACCCCCAACGGTAAACACAAATAGCGCTGGTTTCAgg > 2:6248371/1‑76 (MQ=255) gACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGa < 1:1403689/76‑1 (MQ=255) cTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACg < 2:612827/76‑1 (MQ=255) cTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTTGTACCCGCCACGGGAAACACAAATGGCGCTGGTTTCCGGGCg > 1:5629951/1‑76 (MQ=255) ccAGCGTACGGCAGGTGGTGCCTTGGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGcc < 2:2222143/76‑1 (MQ=255) ccAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGcc > 1:2315506/1‑76 (MQ=255) cAGCGTACGGCAGGTGTTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCa < 2:5509506/76‑1 (MQ=255) cAGCGTACGGCAGGGGGTGCCTTTGGCATTCGTACCCGCAACGGCAAACACAAATTGCGCCGGTTTCAGGATGccc > 2:1828969/1‑75 (MQ=255) aGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCaa < 2:3531564/76‑1 (MQ=255) gCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 2:4544259/76‑1 (MQ=255) gTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAc < 1:632607/76‑1 (MQ=255) tACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcg < 1:7470282/76‑1 (MQ=255) cGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGGAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg > 1:4271549/1‑76 (MQ=255) ggCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCACGACgcgc > 1:3671586/1‑76 (MQ=255) ggCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACgcgc > 1:1118486/1‑76 (MQ=255) aGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 1:2551773/1‑76 (MQ=255) aGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 1:2606152/1‑76 (MQ=255) aGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 1:612858/1‑76 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg < 1:6005283/76‑1 (MQ=255) gtggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa > 1:7211001/1‑76 (MQ=255) | ACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGA > REL606/2376275‑2376422 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |