| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 473,924 | A→C | V461G (GTC→GGC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 473,924 | 0 | A | C | 99.6% | 1059.1 / NA | 257 | V461G (GTC→GGC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (147/109); total (148/109) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 261 total aligned reads displayed.
AGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCG > REL606/473852‑473995 | ccATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCcg < 2:952991/76‑1 (MQ=255) aTTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCcgcg > 2:2781131/1‑76 (MQ=255) aTTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCcgcg > 2:2441296/1‑76 (MQ=255) tttGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGct < 1:2336370/76‑1 (MQ=255) tGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGATCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctct < 2:7043174/76‑1 (MQ=255) gCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCtctc < 2:3939467/76‑1 (MQ=255) cacaTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCg < 1:2127683/76‑1 (MQ=255) cacaTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCg > 2:1734282/1‑76 (MQ=255) acaTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc < 2:2067351/76‑1 (MQ=255) acaTCCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc < 2:121507/76‑1 (MQ=255) caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa < 2:2157134/76‑1 (MQ=255) caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa < 2:1337006/76‑1 (MQ=255) caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa < 1:2701344/76‑1 (MQ=255) aTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAg < 2:1412178/76‑1 (MQ=255) aTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAg < 2:435206/76‑1 (MQ=255) ttCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGc > 1:2878087/1‑76 (MQ=255) cAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtca > 1:6675255/1‑76 (MQ=255) cAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtca > 2:74077/1‑76 (MQ=255) cAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtca > 2:3120200/1‑76 (MQ=255) aGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcat > 2:1153553/1‑76 (MQ=255) aTGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatca > 1:6838829/1‑76 (MQ=255) aTGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatca > 1:736555/1‑76 (MQ=255) aTGCGCCAGTTGCATAATTTCTTCGTCCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatca < 2:3773929/76‑1 (MQ=255) gcgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAac < 2:6844211/76‑1 (MQ=255) gcgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAac < 1:5262566/76‑1 (MQ=255) gcgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAac < 2:2507081/76‑1 (MQ=255) gcgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAac < 2:1886272/76‑1 (MQ=255) cgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAaca > 2:7261599/1‑76 (MQ=255) ccAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACg < 1:4632056/76‑1 (MQ=255) ccAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACg < 2:4052197/76‑1 (MQ=255) cAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACgg < 1:1024297/76‑1 (MQ=255) cAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACgg < 1:4791239/76‑1 (MQ=255) aGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACggg > 2:1590902/1‑76 (MQ=255) tGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTc > 1:1441109/1‑76 (MQ=255) tGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTc > 1:392601/1‑76 (MQ=255) gCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCg < 1:3249466/76‑1 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg < 1:6360439/76‑1 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg > 2:2367461/1‑76 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg > 1:6472355/1‑76 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg > 1:3306754/1‑76 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg > 1:4345930/1‑76 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg < 1:3840409/76‑1 (MQ=255) tAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGa > 1:6210675/1‑76 (MQ=255) tAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGa > 2:3017018/1‑76 (MQ=255) tttCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGt > 2:546392/1‑76 (MQ=255) cttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtctc > 1:7156419/1‑76 (MQ=255) cttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtctc > 2:7078941/1‑76 (MQ=255) tcgttcgCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCaa > 2:4228130/1‑76 (MQ=255) cgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAAt > 1:7417619/1‑76 (MQ=255) cgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAAt > 2:332174/1‑76 (MQ=255) cgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAAt < 1:5601364/76‑1 (MQ=255) gttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATc < 2:4795516/76‑1 (MQ=255) gttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATc < 1:2598351/76‑1 (MQ=255) ttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATca < 1:3029443/76‑1 (MQ=255) ttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATca > 1:1839001/1‑76 (MQ=255) ttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATca < 1:162806/76‑1 (MQ=255) ttcgCCGCATTG‑CCAATACTGCGCGGCCCCCGCGCTCTCGCCGCTCATCAACACGGGGTCGTGACGTCTCAATcac > 2:2296994/1‑76 (MQ=255) tcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcac < 1:2141154/76‑1 (MQ=255) cgccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacc < 1:6574645/76‑1 (MQ=255) gccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacca < 2:7185861/76‑1 (MQ=255) ccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcaccac < 1:1122017/76‑1 (MQ=255) ccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcaccac > 2:2820458/1‑76 (MQ=255) cgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCaccacc > 1:4728782/1‑76 (MQ=255) cgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCaccacc < 1:446681/76‑1 (MQ=255) cgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCaccacc < 2:2879343/76‑1 (MQ=255) gcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCAccacca < 1:918884/76‑1 (MQ=255) gcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCAccacca > 2:3867779/1‑76 (MQ=255) cATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAg < 2:6083670/76‑1 (MQ=255) cATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAg > 1:4054438/1‑76 (MQ=255) aTTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGc > 1:1745586/1‑76 (MQ=255) ttGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCg > 1:1912773/1‑76 (MQ=255) ttGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCg > 2:6528972/1‑76 (MQ=255) tGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCgg < 2:6457566/76‑1 (MQ=255) gCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGa > 2:5221340/1‑76 (MQ=255) gCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGa < 1:2466259/76‑1 (MQ=255) cccAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGaa < 1:3821154/76‑1 (MQ=255) ccAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAat > 1:7039691/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata < 1:2773171/76‑1 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 1:4003934/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 2:7472840/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 1:362393/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 1:4947911/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 2:3207004/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 2:3495004/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 2:540801/1‑76 (MQ=255) aaTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAatat > 2:72953/1‑76 (MQ=255) aaTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAatat > 2:449588/1‑76 (MQ=255) aTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATc > 1:5996779/1‑76 (MQ=255) aTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATc < 1:6334877/76‑1 (MQ=255) tACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCa > 2:4388957/1‑76 (MQ=255) tACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCa > 2:1808824/1‑76 (MQ=255) tACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCa > 1:2276233/1‑76 (MQ=255) cTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCaga > 2:1039866/1‑76 (MQ=255) tGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCagag > 2:5739013/1‑76 (MQ=255) tGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCagag > 2:4755745/1‑76 (MQ=255) tGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCagag < 2:4716977/76‑1 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg > 2:6145987/1‑76 (MQ=255) cgcgGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGc < 2:5033307/76‑1 (MQ=255) cgcgGCCCCCGCGCTCTCCCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGc > 2:3541853/1‑76 (MQ=255) gcgGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCg < 2:3992330/76‑1 (MQ=255) | AGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCG > REL606/473852‑473995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |