Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,193,7790GT100.0% 696.6 / NA 176L143I (CTT→ATT) yeiBconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (64/112);  total (64/112)

Only 100 of 191 total aligned reads displayed.

CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193707‑2193851
                                                                         |                                                                        
ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATccc                                                                       <  2:4606587/76‑1 (MQ=255)
ccGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTG‑TGGGGAATCAGCCCCAAAAACAGCAAAAAGCCCATccc                                                                       >  2:1633744/1‑76 (MQ=255)
 cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                      <  1:2152312/76‑1 (MQ=255)
  gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     <  2:6931202/76‑1 (MQ=255)
  gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     <  2:4554946/76‑1 (MQ=255)
  gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     <  1:7172896/76‑1 (MQ=255)
  gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGTG‑TCGGGAAACAGCCCCCATAACAACAAAAAGCCCAAccccc                                                                     >  1:107656/1‑75 (MQ=255)
   aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac                                                                    <  2:6308600/76‑1 (MQ=255)
   aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac                                                                    <  1:20631/76‑1 (MQ=255)
    tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc                                                                   <  2:6258388/76‑1 (MQ=255)
      caaccGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                 <  1:7502330/73‑1 (MQ=255)
      cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                 <  1:2757621/76‑1 (MQ=255)
       aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                                <  1:3848466/76‑1 (MQ=255)
       aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTG‑TCGGGAAACAGCCCCCAAAAAAGCAAAACGCCCAACCCCACCAGa                                                                >  2:6027279/1‑76 (MQ=255)
        tCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                               <  2:5211813/76‑1 (MQ=255)
         ccGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                              <  1:6587805/76‑1 (MQ=255)
          cGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                             <  1:3901572/76‑1 (MQ=255)
          cGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                             <  1:2868217/76‑1 (MQ=255)
          cGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                             <  2:6709057/76‑1 (MQ=255)
           ggCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                            <  1:6349897/76‑1 (MQ=255)
            gCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                                                           <  2:5410462/76‑1 (MQ=255)
            gCGTCCAGGCGCGGCTGGGCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAAACCCCACCCGAAAAAg                                                           >  1:4366381/1‑76 (MQ=255)
             cGTCCAGGCGCGGCTGGGCTGGCTG‑TCGGAAATCAGCCCCCATAACAGCAAAACGCCAAACCCCCCCAGATAAAGc                                                          >  1:3001556/1‑76 (MQ=255)
              gTCCAGGCGCGGCTGGGCTGGCTGTTGGGAAATCA‑CCCCCATAACAGCAAAACGCCCAACCCCACCCGATAAAACa                                                         >  2:2534728/1‑76 (MQ=255)
               tCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt                                                        <  2:7106520/76‑1 (MQ=255)
               tCCAGGCGCGGCTGGGCTGGCTG‑TCGGGAATCAGCCCCAATAACAGCAAAAAGCCAAACCCCCCCAGATAAAGCAt                                                        >  1:4127584/1‑76 (MQ=255)
                ccAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                       <  1:4430918/76‑1 (MQ=255)
                ccAGGCGCGGCTGGGCTGGCTG‑TCGGAAATCAGCCCCCATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                       >  1:6473083/1‑76 (MQ=255)
                 cAGGCGCGGCTGGGCTGGCCG‑GCGGGAAAAAGCCCCCAAAAAATCAAAAAGCCCATCCCCCCCCAATAAAGCaata                                                      >  2:2685429/1‑73 (MQ=255)
                   ggCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg                                                    <  1:6624352/76‑1 (MQ=255)
                      gcgGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                 <  1:5901514/76‑1 (MQ=255)
                        ggCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               <  2:2313393/76‑1 (MQ=255)
                         gCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              >  1:5691661/1‑76 (MQ=255)
                         gCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              <  1:4657424/76‑1 (MQ=255)
                         gCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              <  2:4373377/76‑1 (MQ=255)
                         gCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              <  1:3963205/76‑1 (MQ=255)
                         gCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              >  2:4116892/1‑76 (MQ=255)
                         gCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              >  1:68443/1‑76 (MQ=255)
                          cTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  1:1231978/76‑1 (MQ=255)
                          cTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             >  1:7016049/1‑76 (MQ=255)
                           tGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  1:3600488/76‑1 (MQ=255)
                           tGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  2:4474577/76‑1 (MQ=255)
                            ggTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  2:2124253/1‑76 (MQ=255)
                             gTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:6395180/76‑1 (MQ=255)
                                tGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  1:7271651/1‑76 (MQ=255)
                                 ggCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      <  1:3216031/76‑1 (MQ=255)
                                 ggCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      <  2:2904152/76‑1 (MQ=255)
                                 ggCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      >  2:2794965/1‑76 (MQ=255)
                                  gCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                     <  1:4165078/76‑1 (MQ=255)
                                  gCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                     >  2:3790067/1‑76 (MQ=255)
                                    tG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  1:4632680/1‑76 (MQ=255)
                                     g‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:6539240/76‑1 (MQ=255)
                                     g‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:5099356/76‑1 (MQ=255)
                                     g‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:181090/76‑1 (MQ=255)
                                     g‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  1:2526947/76‑1 (MQ=255)
                                     g‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  2:7266189/1‑76 (MQ=255)
                                     g‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  2:3095819/1‑76 (MQ=255)
                                     g‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:514692/76‑1 (MQ=255)
                                     g‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:4806617/76‑1 (MQ=255)
                                     g‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  2:3005805/1‑76 (MQ=255)
                                       tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:395422/76‑1 (MQ=255)
                                       tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:6534122/76‑1 (MQ=255)
                                        cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  2:1500791/76‑1 (MQ=255)
                                        cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  1:1428213/76‑1 (MQ=255)
                                        cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  2:1755065/1‑76 (MQ=255)
                                        cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  1:583269/76‑1 (MQ=255)
                                         ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:7151598/76‑1 (MQ=255)
                                         ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               >  1:1720910/1‑76 (MQ=255)
                                         ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  1:5616624/76‑1 (MQ=255)
                                         ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  1:6284848/76‑1 (MQ=255)
                                          gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:909311/76‑1 (MQ=255)
                                           aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  2:5567419/76‑1 (MQ=255)
                                           aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  2:7464949/1‑76 (MQ=255)
                                           aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  1:2392812/76‑1 (MQ=255)
                                            aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            <  1:2769793/76‑1 (MQ=255)
                                              tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          >  2:2551639/1‑76 (MQ=255)
                                               cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         >  1:738449/1‑76 (MQ=255)
                                               cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:765589/76‑1 (MQ=255)
                                                 gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       <  1:3769100/76‑1 (MQ=255)
                                                  ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      <  2:4681285/76‑1 (MQ=255)
                                                    ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  1:5417043/1‑76 (MQ=255)
                                                     cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                   >  1:7457899/1‑76 (MQ=255)
                                                      aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  2:7026068/1‑76 (MQ=255)
                                                       aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  2:1066532/1‑76 (MQ=255)
                                                       aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  1:5275335/1‑76 (MQ=255)
                                                       aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  1:5035607/1‑76 (MQ=255)
                                                         aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               <  2:5622666/76‑1 (MQ=255)
                                                         aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  2:585473/1‑76 (MQ=255)
                                                          aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg              >  2:139459/1‑76 (MQ=255)
                                                            aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa            <  2:4781991/76‑1 (MQ=255)
                                                             gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  1:875185/76‑1 (MQ=255)
                                                               aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:377937/76‑1 (MQ=255)
                                                               aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  2:335300/1‑76 (MQ=255)
                                                                 aaagCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:3693100/4‑76 (MQ=255)
                                                                 aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:6147818/1‑76 (MQ=255)
                                                                 aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:6809816/1‑76 (MQ=255)
                                                                  aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      >  2:127711/1‑76 (MQ=255)
                                                                  aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      >  2:2179570/1‑76 (MQ=255)
                                                                      cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:6107493/1‑76 (MQ=255)
                                                                      cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:6664491/1‑76 (MQ=255)
                                                                         |                                                                        
CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193707‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑