| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,762,213 | T→G | Y67S (TAT→TCT) | rpoS ← | RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,762,213 | 0 | T | G | 99.2% | 1019.2 / ‑3.4 | 238 | Y67S (TAT→TCT) | rpoS | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base G (136/100); total (138/100) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 247 total aligned reads displayed.
GCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCT > REL606/2762140‑2762285 | gcgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTgaagaa < 1:3526046/76‑1 (MQ=255) cgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAAc > 2:6027470/1‑76 (MQ=255) cgAGAGGCGACATCTCCACGCAGGGCGTGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGCGGTGAAGAAc > 1:7152923/1‑76 (MQ=255) gagaGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAAcc < 2:2782214/76‑1 (MQ=255) gagaGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAAcc < 1:6216609/76‑1 (MQ=255) agagGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCa > 2:5755905/1‑76 (MQ=255) gagGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCaa > 1:5664142/1‑76 (MQ=255) gagGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCaa > 1:2558758/1‑76 (MQ=255) agGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAAt > 1:5225506/1‑76 (MQ=255) agGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAt < 2:4848616/76‑1 (MQ=255) gCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAtct < 2:4379516/76‑1 (MQ=255) gCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAtct < 1:3868156/76‑1 (MQ=255) cGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAtctc < 1:2226240/76‑1 (MQ=255) cGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAtctc > 1:3371453/1‑76 (MQ=255) cGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAAtctc > 1:2313019/1‑76 (MQ=255) gACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCa < 1:3578036/76‑1 (MQ=255) gACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCa < 1:5007641/76‑1 (MQ=255) gACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCa < 1:2287135/76‑1 (MQ=255) cATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCAca > 2:5448822/1‑75 (MQ=255) aTCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCa < 1:6636731/76‑1 (MQ=255) tcCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAgg < 2:2926062/76‑1 (MQ=255) tcCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAgg > 2:7393813/1‑76 (MQ=255) ccACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGt > 2:6938358/1‑76 (MQ=255) cACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTa < 2:2351880/76‑1 (MQ=255) aCGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTaa < 2:1131120/76‑1 (MQ=255) cGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTaaa < 1:6724648/76‑1 (MQ=255) cGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTaaa > 1:5376181/1‑76 (MQ=255) gCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTCAAg > 2:7269783/1‑76 (MQ=255) gCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAg > 1:4910967/1‑76 (MQ=255) gCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAg > 2:822081/1‑76 (MQ=255) cAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGc > 2:3315463/1‑76 (MQ=255) cAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGc > 2:1190058/1‑76 (MQ=255) cAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGCAAAGc > 2:7372310/1‑76 (MQ=255) aGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCt < 1:3135283/76‑1 (MQ=255) gTGCGCGACGCGCAAAATAAACTTCTTCTTCGGTCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTg < 2:3037763/76‑1 (MQ=255) gTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTg < 1:1725711/76‑1 (MQ=255) gTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTg < 2:5780837/76‑1 (MQ=255) gTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTg < 2:3433203/76‑1 (MQ=255) cgcgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGt > 2:5141553/1‑76 (MQ=255) cgcgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGt < 1:2798545/76‑1 (MQ=255) gcgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTc < 1:4173905/76‑1 (MQ=255) aCGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTcgcg < 1:1410243/76‑1 (MQ=255) aCGCGCAAAATAAACTTCTTCTCCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTcgcg < 1:4557648/76‑1 (MQ=255) gcgcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTc > 1:1094695/1‑76 (MQ=255) gcgcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTc > 1:6806813/1‑76 (MQ=255) gcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCa > 2:5800030/1‑76 (MQ=255) aaaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAac > 2:366074/1‑76 (MQ=255) aaaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAac > 2:7078107/1‑76 (MQ=255) aaaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAac > 1:2887162/1‑76 (MQ=255) aaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAaca > 2:3852154/1‑76 (MQ=255) aaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAaca < 1:3919470/76‑1 (MQ=255) aaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacac > 2:3291613/1‑76 (MQ=255) aaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacac < 1:5145496/76‑1 (MQ=255) aaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacac < 1:7045278/76‑1 (MQ=255) aaaCTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACg < 2:4169055/76‑1 (MQ=255) aaaCTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACg > 2:3153051/1‑76 (MQ=255) aaaCTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACg > 2:6820030/1‑76 (MQ=255) aaCTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGc < 2:1653097/76‑1 (MQ=255) aCTTCTTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCt > 2:6174019/1‑76 (MQ=255) cttcttcttCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtg > 1:4503186/1‑76 (MQ=255) tcttcttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgtg < 2:1440025/76‑1 (MQ=255) cttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGc > 2:4318006/1‑76 (MQ=255) cttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGc > 1:3272872/1‑76 (MQ=255) cttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGc > 1:5813595/1‑76 (MQ=255) ttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCt > 2:3552839/1‑76 (MQ=255) ttcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCt < 2:6358138/76‑1 (MQ=255) tcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTc > 1:1973345/1‑76 (MQ=255) tcGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTc > 1:1166802/1‑76 (MQ=255) cGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTcc > 1:4317404/1‑76 (MQ=255) gCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCt > 2:2837899/1‑76 (MQ=255) gCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCt < 2:5900728/76‑1 (MQ=255) ccGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTg < 2:4535836/76‑1 (MQ=255) cGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGc < 1:4759415/76‑1 (MQ=255) cGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGc > 2:6959972/1‑76 (MQ=255) gTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCttgg > 2:3738862/1‑73 (MQ=255) gTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCg > 1:3198749/1‑76 (MQ=255) gTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCg < 1:1015854/76‑1 (MQ=255) ttAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGa > 1:1785966/1‑76 (MQ=255) ttAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGa > 1:5550111/1‑76 (MQ=255) ttAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGa > 1:2133143/1‑76 (MQ=255) tAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGAt > 2:2230044/1‑76 (MQ=255) tAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGAt > 2:2622288/1‑76 (MQ=255) tAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGAt > 2:1281423/1‑76 (MQ=255) tAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGACCAACACACGCTGTGTGGCTCCCTGCGAt < 2:7505290/76‑1 (MQ=255) aaCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa > 2:1716306/1‑76 (MQ=255) aaCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa > 2:6723273/1‑76 (MQ=255) aaCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa > 2:4791299/1‑76 (MQ=255) aaCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa < 1:5949366/76‑1 (MQ=255) aaCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa < 1:2635876/76‑1 (MQ=255) aCAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATaa < 2:2522535/76‑1 (MQ=255) cAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAAc < 1:6819739/76‑1 (MQ=255) cAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAAc < 1:2961920/76‑1 (MQ=255) aGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACa < 1:1031929/76‑1 (MQ=255) aGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACa < 1:193221/76‑1 (MQ=255) aGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACa < 2:3215832/76‑1 (MQ=255) gtggtgAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAg < 1:871294/76‑1 (MQ=255) tggtgAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGt > 2:1997548/1‑76 (MQ=255) tggtgAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGt < 1:938621/76‑1 (MQ=255) ggtgAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGtt < 2:246654/76‑1 (MQ=255) gaagaaCCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCct < 2:3919538/76‑1 (MQ=255) | GCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCT > REL606/2762140‑2762285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |