Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 4,163,752 T→G I966S (ATC→AGC)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,163,7520TG100.0% 665.7 / NA 157I966S (ATC→AGC) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (109/48);  total (109/48)

Only 100 of 191 total aligned reads displayed.

GACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163679‑4163824
                                                                         |                                                                         
 aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt                                                                        <  2:6328809/76‑1 (MQ=255)
 aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt                                                                        <  1:302106/76‑1 (MQ=255)
  cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc                                                                       <  1:1035453/76‑1 (MQ=255)
  cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc                                                                       <  2:6191926/76‑1 (MQ=255)
  cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc                                                                       >  1:3049637/1‑76 (MQ=255)
   aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCttg                                                                      <  2:4604051/76‑3 (MQ=255)
   aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                      >  2:1349684/1‑76 (MQ=255)
   aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                      <  1:4936576/76‑1 (MQ=255)
   aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                      <  2:2016507/76‑1 (MQ=255)
   aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                      >  2:2938991/1‑76 (MQ=255)
   aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                      >  2:3358533/1‑76 (MQ=255)
   aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                      >  2:1638942/1‑76 (MQ=255)
    aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGa                                                                     <  2:3026057/76‑1 (MQ=255)
      cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑g                                                                  <  1:5695331/76‑1 (MQ=255)
      cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑g                                                                  >  1:1341247/1‑76 (MQ=255)
      cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑g                                                                  >  1:3151250/1‑76 (MQ=255)
       gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑Gc                                                                 <  1:2078669/76‑1 (MQ=255)
         gcgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCgg                                                               >  2:4586703/1‑76 (MQ=255)
             tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCt                                                           <  1:2023572/76‑1 (MQ=255)
             tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCt                                                           >  1:2382314/1‑76 (MQ=255)
             tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCt                                                           >  1:5742938/1‑76 (MQ=255)
              ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTg                                                          <  2:3168578/76‑1 (MQ=255)
                aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGtt                                                        <  2:7459916/76‑1 (MQ=255)
                 aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTc                                                       <  1:7056970/76‑1 (MQ=255)
                  aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCa                                                      <  2:4024167/76‑1 (MQ=255)
                   tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAg                                                     <  1:5376093/76‑1 (MQ=255)
                   tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAg                                                     <  2:836081/76‑1 (MQ=255)
                    cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGc                                                    >  2:1702516/1‑76 (MQ=255)
                    cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGc                                                    <  2:1783239/76‑1 (MQ=255)
                    cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGc                                                    >  2:2827991/1‑76 (MQ=255)
                     gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGcc                                                   >  1:6034377/1‑76 (MQ=255)
                      aagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCg                                                  >  2:5090948/1‑76 (MQ=255)
                      aagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCg                                                  >  2:706419/1‑76 (MQ=255)
                        gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTa                                                >  2:5569412/1‑76 (MQ=255)
                        gaaAGGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTa                                                <  1:2997897/76‑1 (MQ=255)
                         aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTAt                                               >  1:6897467/1‑76 (MQ=255)
                         aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTAt                                               >  1:3286932/1‑76 (MQ=255)
                         aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCGGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTAt                                               <  2:7196948/76‑1 (MQ=255)
                         aaaTGCAGCGCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTAt                                               <  1:6732131/76‑1 (MQ=255)
                          aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATc                                              >  2:6733587/1‑76 (MQ=255)
                          aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATc                                              >  1:1579467/1‑76 (MQ=255)
                           aTGCAGCTCAAACAGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATcc                                             <  2:5478987/76‑1 (MQ=255)
                           aTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATcc                                             >  2:6940427/1‑76 (MQ=255)
                           aTGCAGCTAAAACGGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATcc                                             <  2:2308976/76‑1 (MQ=255)
                           aTGCAGCTAAAACAGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATcc                                             <  2:207175/76‑1 (MQ=255)
                             gcagcaAAAAGAGGCAAAAAAAGCCCTGTCTAAAAAACTGCAGCGCCTCGAG‑GGGGGTCTTTTCAGCCGTATCCgt                                           <  2:1461301/76‑1 (MQ=255)
                              cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCgtg                                          >  2:3670475/1‑76 (MQ=255)
                              cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCgtg                                          >  1:4372793/1‑76 (MQ=255)
                              cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCgtg                                          >  2:519132/1‑76 (MQ=255)
                              cAGCTAAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCgtg                                          <  1:3473543/76‑1 (MQ=255)
                                gCTAAAACAGGCGAAGAAAGCCCTGTCGGAAGACCTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCgtgct                                        <  2:2460003/76‑1 (MQ=255)
                                gCTAAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCgtgct                                        <  1:5262093/76‑1 (MQ=255)
                                 ctaaaaCAGGCAAAGAAAGCCCGGTCTGAAAAACTGCAGAGCCTCGAA‑GCGGGTTTGTTCAGCCGTATCCgtgctg                                       <  2:853520/73‑1 (MQ=255)
                                  tCAAACAGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCgtgctgt                                      <  1:5175684/76‑1 (MQ=255)
                                  tCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCgtgctgt                                      >  1:6216672/1‑76 (MQ=255)
                                    aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCgtgctgtgc                                    <  1:7377367/76‑1 (MQ=255)
                                     aaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCgtgctgtgct                                   <  1:6329527/76‑1 (MQ=255)
                                      aCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGGATGTTCAGCCGAATCCGtgctgtgctg                                  >  2:3739191/1‑76 (MQ=255)
                                       cAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTgg                                 >  2:5602440/1‑76 (MQ=255)
                                         ggCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTa                               <  1:1072545/76‑1 (MQ=255)
                                         ggCGAAAAAAGCCCTGTCTGAAGACCTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTa                               <  2:7108490/76‑1 (MQ=255)
                                          ggaaagaaggcCCTGTCTGAAAAACGGCAGAGCCTCGAA‑GCGGGTCTGTCCAGCCGTATCCGTGCTGTGCTGGTAg                              <  2:6064812/73‑1 (MQ=255)
                                          gaaaagaaagcCCTGTCTAAAGACCTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg                              <  2:5206403/73‑1 (MQ=255)
                                          gCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg                              <  1:5495123/76‑1 (MQ=255)
                                           caaagaaagcCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGc                             <  1:4630234/74‑1 (MQ=255)
                                           cGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGc                             <  1:3975801/76‑1 (MQ=255)
                                            gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGcc                            >  2:2023564/1‑76 (MQ=255)
                                             aagaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg                           >  2:3881818/1‑76 (MQ=255)
                                               gaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCggg                         >  1:7002876/1‑75 (MQ=255)
                                                aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        >  1:982700/1‑76 (MQ=255)
                                                aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        >  2:4288621/1‑76 (MQ=255)
                                                aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        >  1:2857555/1‑76 (MQ=255)
                                                aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        >  2:1157736/1‑76 (MQ=255)
                                                 aagaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       >  2:6014092/1‑76 (MQ=255)
                                                 aagaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       >  2:5761621/1‑76 (MQ=255)
                                                 aagaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       >  2:4865804/1‑76 (MQ=255)
                                                 aagaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       >  2:1067380/1‑76 (MQ=255)
                                                 aagaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       >  2:755552/1‑76 (MQ=255)
                                                  agaCCTGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc                      >  1:6116072/1‑76 (MQ=255)
                                                    cccTGTCTGAAGAACTGCAGAGCCGCAAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGt                    <  1:2743549/75‑1 (MQ=255)
                                                       tttCTGAAAAACTGCAGGGCCCCGAACGGGGGTTTGTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg                  <  2:5436036/74‑1 (MQ=255)
                                                       tGTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGGGTTGa                 >  1:2577287/1‑76 (MQ=255)
                                                        gTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATGCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa                >  2:6847342/1‑76 (MQ=255)
                                                        gTCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa                >  1:6779914/1‑76 (MQ=255)
                                                         tCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg               >  1:91013/1‑76 (MQ=255)
                                                         tCTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg               >  2:1996503/1‑76 (MQ=255)
                                                          cTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  1:7481910/1‑76 (MQ=255)
                                                          cTGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  2:2652774/1‑76 (MQ=255)
                                                           tGAAGAACTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt             >  2:673980/1‑76 (MQ=255)
                                                           ggaaaaaCTGCAGAGCCTGGAA‑GGGGGTCTTTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt             <  1:6518731/75‑1 (MQ=255)
                                                            gaagaaCTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            >  2:7331681/1‑76 (MQ=255)
                                                             aagacCTGCGGAGCCTGGAG‑GCGGTTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga           <  1:4214493/76‑1 (MQ=255)
                                                             aagaaCTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga           >  2:6516968/1‑76 (MQ=255)
                                                             aagaaCTGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga           >  1:7272193/1‑76 (MQ=255)
                                                              agacCTGCAGGCCCTGAAG‑GCGGTTCGTTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag          <  1:2215376/76‑1 (MQ=255)
                                                                aaCTGCAGAGCCTCGAA‑GCGGGTCTGTTTAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGaa        >  1:6647556/1‑76 (MQ=255)
                                                                aaCGGCAGGCCCTGGAG‑GGGGGTTTGTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGaa        <  1:5872293/76‑1 (MQ=255)
                                                                 ccTGCAGGCCCTCGAG‑GGGGGTCTGTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg       <  1:4879459/75‑1 (MQ=255)
                                                                   tGCAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     >  1:2464485/1‑76 (MQ=255)
                                                                     cAGAGCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg   >  2:5948310/1‑76 (MQ=255)
                                                                         |                                                                         
GACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAA‑GCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163679‑4163824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑