BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	649,601	T→C	I401V (ATC→GTC)	mrdA ←	transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	649,601	0	T	C	100.0%	779.6 / NA	182	I401V (ATC→GTC)	mrdA	transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (78/104); total (78/104)

Only 100 of 208 total aligned reads displayed.

GTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACG  >  REL606/649529‑649673

gTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGAcccc                                                                       <  1:2357317/76‑1 (MQ=255)

 ttcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACcccc                                                                      <  1:3879144/76‑1 (MQ=255)

 ttcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACcccc                                                                      >  1:4780/1‑76 (MQ=255)

  tcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCa                                                                     >  2:6822450/1‑76 (MQ=255)

  tcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCa                                                                     <  1:6542743/76‑1 (MQ=255)

  tcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCa                                                                     >  1:4034626/1‑76 (MQ=255)

  tcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCa                                                                     >  1:6887464/1‑76 (MQ=255)

  tcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCa                                                                     <  1:4118996/76‑1 (MQ=255)

   cttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCat                                                                    >  2:5644006/1‑76 (MQ=255)

    ttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCata                                                                   <  1:3015951/76‑1 (MQ=255)

    ttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCata                                                                   <  1:6999262/76‑1 (MQ=255)

      ccGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATc                                                                 <  2:604172/76‑1 (MQ=255)

      ccGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATc                                                                 >  1:5488872/1‑76 (MQ=255)

      ccGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATc                                                                 <  2:3236409/76‑1 (MQ=255)

        gCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGt                                                               <  2:1352035/76‑1 (MQ=255)

        gCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGt                                                               <  1:373419/76‑1 (MQ=255)

        gCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGt                                                               <  1:3816826/76‑1 (MQ=255)

        gCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGt                                                               <  2:1555104/76‑1 (MQ=255)

         ccAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTa                                                              >  1:6088751/1‑76 (MQ=255)

          cAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAg                                                             <  1:6953357/76‑1 (MQ=255)

          cAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAg                                                             <  1:2573237/76‑1 (MQ=255)

           aGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAgg                                                            <  1:4263955/76‑1 (MQ=255)

            ggTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGc                                                           <  1:6207515/76‑1 (MQ=255)

             gTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGcc                                                          <  1:2742017/76‑1 (MQ=255)

               cGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCAc                                                        <  2:5856382/76‑1 (MQ=255)

                gATACCGGTGTAATGACCATAACCGAATTTACCCATCTATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCAcc                                                       >  1:6677370/1‑76 (MQ=255)

                 aTACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCt                                                      <  1:408435/76‑1 (MQ=255)

                 aTACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCt                                                      <  2:1128488/76‑1 (MQ=255)

                  tACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTg                                                     <  2:3807225/76‑1 (MQ=255)

                  tACCGGTGTAAGGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGCCCCCCATATCGTAGGCCACCTg                                                     <  1:3348223/76‑1 (MQ=255)

                   aCCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGa                                                    >  2:723691/1‑76 (MQ=255)

                   aCCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGa                                                    <  2:6737862/76‑1 (MQ=255)

                    ccGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGAt                                                   <  2:3687085/76‑1 (MQ=255)

                    ccGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGAt                                                   <  1:3757450/76‑1 (MQ=255)

                     cGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATa                                                  <  1:3508773/76‑1 (MQ=255)

                     cGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATa                                                  <  1:5683288/76‑1 (MQ=255)

                     cGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATa                                                  <  1:5825284/76‑1 (MQ=255)

                      ggTGTAATGACCATAACCGAATTTACCTATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATag                                                 <  1:2177573/76‑1 (MQ=255)

                      ggTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATag                                                 <  1:4431719/76‑1 (MQ=255)

                      ggTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATag                                                 <  1:6019686/76‑1 (MQ=255)

                       gtgtAATGACCATACCCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATaga                                                <  1:63093/76‑1 (MQ=255)

                        tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaa                                               >  1:5261561/1‑76 (MQ=255)

                        tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaa                                               <  1:2820759/76‑1 (MQ=255)

                        tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaa                                               >  2:1782913/1‑76 (MQ=255)

                        ggtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaa                                               <  1:203195/75‑1 (MQ=255)

                         gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaag                                              <  2:912287/76‑1 (MQ=255)

                          tAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaaga                                             >  2:4100455/1‑76 (MQ=255)

                          tAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaaga                                             <  1:4987383/76‑1 (MQ=255)

                           aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAgaagaa                                            >  2:2688834/1‑76 (MQ=255)

                             tGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAgg                                          >  1:712072/1‑76 (MQ=255)

                                ccATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTAt                                       >  2:2233765/1‑76 (MQ=255)

                                 cATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATc                                      >  2:5084260/1‑76 (MQ=255)

                                  aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATcc                                     >  1:3702057/1‑76 (MQ=255)

                                   tAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCg                                    >  2:6489766/1‑76 (MQ=255)

                                   tAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCg                                    >  1:7046394/1‑76 (MQ=255)

                                    agccGAATTTACCCATCCATTCAGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGc                                   <  2:2953213/74‑1 (MQ=255)

                                    aaCCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATcgtg                                   <  2:498952/76‑4 (MQ=255)

                                    aaCCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGc                                   >  2:2161700/1‑76 (MQ=255)

                                      ccGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAg                                 >  2:1225469/1‑76 (MQ=255)

                                       cGAATTTCCCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGa                                <  2:4788215/76‑1 (MQ=255)

                                        gAATTTCCCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAt                               <  2:5934688/76‑1 (MQ=255)

                                         aaTTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt                              >  1:6136318/1‑76 (MQ=255)

                                            ttACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttctt                           <  2:3353461/76‑1 (MQ=255)

                                             tccccATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttcttc                          <  1:4443859/74‑1 (MQ=255)

                                             tccccATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttcttc                          <  2:2441374/74‑1 (MQ=255)

                                             tACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttcttc                          >  1:4235692/1‑76 (MQ=255)

                                              aCCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTcc                         >  2:4373339/1‑76 (MQ=255)

                                              aCCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTcc                         >  2:1109514/1‑76 (MQ=255)

                                              aCCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTcc                         <  1:1202078/76‑1 (MQ=255)

                                               cccATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTccc                        >  2:4034841/1‑75 (MQ=255)

                                               cccATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCa                        <  2:5342506/76‑1 (MQ=255)

                                               cccATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCa                        <  1:3251280/76‑1 (MQ=255)

                                                 catccatGCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGc                      <  2:2090232/76‑1 (MQ=255)

                                                  atccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCg                     >  1:3061884/1‑76 (MQ=255)

                                                  atccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCg                     <  1:5579934/76‑1 (MQ=255)

                                                   tccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGa                    <  1:1521102/76‑1 (MQ=255)

                                                    ccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt                   <  2:4483511/76‑1 (MQ=255)

                                                    ccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt                   >  1:5707788/1‑76 (MQ=255)

                                                     catTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATc                  >  2:3576991/1‑76 (MQ=255)

                                                      atTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCt                 <  1:4642752/76‑1 (MQ=255)

                                                      atTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCt                 >  2:5394507/1‑76 (MQ=255)

                                                      atTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCt                 >  2:5470596/1‑76 (MQ=255)

                                                        tCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTtg               >  1:4752154/1‑76 (MQ=255)

                                                         cGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTtgt              >  2:3034468/1‑76 (MQ=255)

                                                          ggAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTtgtg             >  1:755310/1‑76 (MQ=255)

                                                           gagaGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGa            <  2:308368/76‑1 (MQ=255)

                                                           gagaCGCGATCCACCCCCATATCGTAGGCGACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGa            <  2:6212619/76‑1 (MQ=255)

                                                            agagGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCCGATTCTTCCCGCGATCTTGTGAc           >  2:2859694/1‑76 (MQ=255)

                                                             gagGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACa          <  2:7433603/76‑1 (MQ=255)

                                                             gagGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACa          <  1:5588230/76‑1 (MQ=255)

                                                             gagGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACa          <  1:5020989/76‑1 (MQ=255)

                                                               ggCGATCGCCCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACAtt        <  1:5129014/76‑1 (MQ=255)

                                                               ggCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACAtt        <  1:4674984/76‑1 (MQ=255)

                                                               ggCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACAtt        <  2:753526/76‑1 (MQ=255)

                                                                gCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTc       <  1:4837912/76‑1 (MQ=255)

                                                                gCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTc       >  2:4406488/1‑76 (MQ=255)

                                                                 cgatcgacCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCa      <  2:5989682/76‑1 (MQ=255)

                                                                  gatcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAg     <  1:1145712/76‑1 (MQ=255)

                                                                   atcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGa    >  1:2569248/1‑76 (MQ=255)

                                                                    tcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGAc   <  1:4545763/76‑1 (MQ=255)

GTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACG  >  REL606/649529‑649673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`