BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,509	T→G	D244A (GAC→GCC)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,509	0	T	G	100.0%	747.5 / NA	181	D244A (GAC→GCC)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (68/113); total (68/113)

Only 100 of 188 total aligned reads displayed.

CGATGGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGT  >  REL606/1305435‑1305581

cGATGGGGCCTTCCATACGCGCCATCAGATCAAT‑CATTGCCCTACGCCCGCATCTTGGTTGAAGTAGCGAGGGGCg                                                                        >  2:4275109/1‑76 (MQ=255)

  aTGGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGa                                                                       >  2:494148/1‑76 (MQ=255)

   tGGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGAc                                                                      >  2:3200498/1‑76 (MQ=255)

    gggcCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGAcc                                                                     <  2:6038133/76‑1 (MQ=255)

     gccccTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCa                                                                    <  2:6972962/73‑1 (MQ=255)

      ccccTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCat                                                                   <  2:1949851/74‑1 (MQ=255)

      ccccTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCat                                                                   <  1:2222852/74‑1 (MQ=255)

      ccccTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGAGACCat                                                                   <  1:6676186/74‑1 (MQ=255)

        ccTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCatat                                                                 <  1:72113/76‑1 (MQ=255)

         cTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATAtt                                                                >  1:1781007/1‑76 (MQ=255)

          ttCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTc                                                               >  1:2996349/1‑76 (MQ=255)

           tCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGGAGTAGCGAGGGGCGACCATATTCa                                                              <  2:2928917/76‑1 (MQ=255)

           tCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCa                                                              <  1:5089054/76‑1 (MQ=255)

           tCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCa                                                              <  2:2471917/76‑1 (MQ=255)

            ccATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCAt                                                             <  2:3287647/76‑1 (MQ=255)

              aTACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGTGGCGACCATATTCAtgc                                                           >  2:5088452/1‑76 (MQ=255)

               tACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGTCCATATTCAtgct                                                          <  1:1365846/76‑1 (MQ=255)

               tACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCAtgct                                                          <  2:7179883/76‑1 (MQ=255)

               tACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCAtgct                                                          <  1:5399314/76‑1 (MQ=255)

               tACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCAtgct                                                          >  2:6888570/1‑76 (MQ=255)

               tACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCAtgct                                                          <  2:6115878/76‑1 (MQ=255)

                aCGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCAtgctg                                                         <  2:552560/76‑1 (MQ=255)

                 cgcgCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCAtgctgc                                                        <  1:5026769/76‑1 (MQ=255)

                 cgcgCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCAtgctgc                                                        <  2:4739869/76‑1 (MQ=255)

                  gcgcCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGTGACCATATTCATGCTGcc                                                       <  2:3526129/76‑1 (MQ=255)

                  gcgcCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGcc                                                       <  2:6665624/76‑1 (MQ=255)

                    gcCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCgg                                                     <  2:4393465/76‑1 (MQ=255)

                     ccATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGgt                                                    <  2:1658407/76‑1 (MQ=255)

                     ccAGCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGgt                                                    <  2:7231011/76‑1 (MQ=255)

                      cATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGgtg                                                   >  1:7486374/1‑76 (MQ=255)

                      cATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGgtg                                                   <  2:5172701/76‑1 (MQ=255)

                       aTCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGgtgt                                                  >  2:4445786/1‑76 (MQ=255)

                       aTCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGgtgt                                                  <  2:3787964/76‑1 (MQ=255)

                       aTCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGgtgt                                                  <  2:545508/76‑1 (MQ=255)

                        tCAGATAAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTa                                                 <  2:1493021/76‑1 (MQ=255)

                         cAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTAc                                                <  2:1018943/76‑1 (MQ=255)

                         cAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTAc                                                <  1:4302811/76‑1 (MQ=255)

                         cAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTAc                                                >  1:5819699/1‑76 (MQ=255)

                         cAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTAc                                                <  2:5013457/76‑1 (MQ=255)

                         cAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTAc                                                <  1:451137/76‑1 (MQ=255)

                         cAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTAc                                                <  1:3611136/76‑1 (MQ=255)

                          ataTCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTAcg                                               >  2:4657271/3‑76 (MQ=255)

                          aGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTAcg                                               <  2:2993568/76‑1 (MQ=255)

                          aGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTAcg                                               <  2:2971162/76‑1 (MQ=255)

                           gATCATTCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTAcgc                                              <  1:1266680/76‑1 (MQ=255)

                            aTCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTAcgcg                                             >  1:1004049/1‑76 (MQ=255)

                             tCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGa                                            >  1:7308774/1‑76 (MQ=255)

                             tCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGa                                            <  2:5366078/76‑1 (MQ=255)

                              cAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGAt                                           <  1:6724409/76‑1 (MQ=255)

                              cAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGAACATATTCATGCTGCCGGTGTACGCGAt                                           >  2:6216993/1‑76 (MQ=255)

                               aaTCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATg                                          <  1:5051226/76‑1 (MQ=255)

                               aaTCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATg                                          >  1:2176724/1‑76 (MQ=255)

                                aTCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGt                                         <  1:2789457/76‑1 (MQ=255)

                                 tCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTa                                        >  2:3948977/1‑76 (MQ=255)

                                 tCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTa                                        <  2:6971708/76‑1 (MQ=255)

                                   cATTTCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAAt                                      <  1:4264075/76‑1 (MQ=255)

                                   cATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAAt                                      <  2:2165869/76‑1 (MQ=255)

                                    aTTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAAtt                                     <  2:4041012/76‑1 (MQ=255)

                                    aTTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAAtt                                     <  2:5923966/76‑1 (MQ=255)

                                      tGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTAt                                   >  1:2714214/1‑76 (MQ=255)

                                       gCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATc                                  >  1:151285/1‑76 (MQ=255)

                                       gCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATc                                  >  2:343749/1‑76 (MQ=255)

                                          cTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgat                               >  2:2408216/1‑76 (MQ=255)

                                          cTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgat                               >  1:85811/1‑76 (MQ=255)

                                          cTACGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgat                               >  2:7142750/1‑76 (MQ=255)

                                            aCGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatca                             >  1:1807693/1‑76 (MQ=255)

                                             cgcccgccTCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcat                            <  2:5273544/76‑1 (MQ=255)

                                             cacccGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcat                            <  2:7112518/74‑1 (MQ=255)

                                             cGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcat                            <  2:875110/76‑1 (MQ=255)

                                             cGCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcat                            <  1:1707694/76‑1 (MQ=255)

                                              gCCCGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcatg                           >  2:131570/1‑76 (MQ=255)

                                                ccGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcatgat                         <  1:5301504/76‑1 (MQ=255)

                                                ccGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcatgat                         <  2:2679479/76‑1 (MQ=255)

                                                ccGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcatgat                         <  1:6930536/76‑1 (MQ=255)

                                                ccGCATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcatgat                         <  2:6435080/76‑1 (MQ=255)

                                                   cATCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcatgatcat                      >  1:4463976/1‑76 (MQ=255)

                                                   cATCTGGCTTGAAGTAGGGAGGGGCGGCCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcatgatcat                      <  1:5241280/76‑1 (MQ=255)

                                                    aTCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGatcatc                     >  1:3118603/1‑76 (MQ=255)

                                                     tCTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCt                    >  2:7080493/1‑76 (MQ=255)

                                                      cTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCtt                   <  1:4861165/76‑1 (MQ=255)

                                                      cTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCtt                   <  1:2114068/76‑1 (MQ=255)

                                                      cTTGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCtt                   <  1:3281372/76‑1 (MQ=255)

                                                       ttGTTTGAAGTAGGGAGGGGCGACCATATTCATGCTG‑CGGTGTACGCGATGTAATTATCGATCATGATCATCTTGc                 <  1:3423791/76‑1 (MQ=255)

                                                       ttGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTg                  >  2:6773682/1‑76 (MQ=255)

                                                       ttGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTg                  <  2:6630201/76‑1 (MQ=255)

                                                        tGTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGc                 <  1:3436418/76‑1 (MQ=255)

                                                         gTTTGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCg                >  2:6300981/1‑76 (MQ=255)

                                                          tttGAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGa               >  2:7316882/1‑76 (MQ=255)

                                                             gAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGt            >  2:5275509/1‑76 (MQ=255)

                                                             gAAGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGt            >  1:4772001/1‑76 (MQ=255)

                                                              aaGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGtt           <  1:3354595/76‑1 (MQ=255)

                                                               aGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTg          <  1:3903000/76‑1 (MQ=255)

                                                               aGTAGCGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTg          <  1:4038156/76‑1 (MQ=255)

                                                                    cGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCa     >  2:6297200/1‑76 (MQ=255)

                                                                    cGAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCa     <  1:7376900/76‑1 (MQ=255)

                                                                     gAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAg    >  1:5675677/1‑76 (MQ=255)

                                                                     gAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAg    <  1:6838128/76‑1 (MQ=255)

                                                                     gAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAg    <  2:4381520/76‑1 (MQ=255)

                                                                     gAGGGGCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAg    <  1:5560766/76‑1 (MQ=255)

                                                                      gggggCGACCGTATGCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAgg   <  2:2241823/75‑1 (MQ=255)

CGATGGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGT  >  REL606/1305435‑1305581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`