| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,164,917 | A→C | S309A (TCG→GCG) | yehU ← | predicted sensory kinase in two‑component system with YehT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,164,917 | 0 | A | C | 99.5% | 817.9 / ‑3.9 | 189 | S309A (TCG→GCG) | yehU | predicted sensory kinase in two‑component system with YehT |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (84/104); total (84/105) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
Only 100 of 194 total aligned reads displayed.
TTTTTGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACTTCACC > REL606/2164844‑2164989 | ttttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGcccc > 1:2601219/1‑76 (MQ=255) tttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCcccc > 1:402690/1‑76 (MQ=255) tttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCcccc < 2:6215995/76‑1 (MQ=255) ttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCa > 2:495166/1‑76 (MQ=255) ttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGCCCCCa < 2:3982028/76‑1 (MQ=255) tGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAg > 2:6585289/1‑76 (MQ=255) gCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGtt > 1:4202893/1‑76 (MQ=255) cTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGttt < 2:7164360/76‑1 (MQ=255) tCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGc > 2:591422/1‑76 (MQ=255) tCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGc < 1:7400928/76‑1 (MQ=255) cATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCa < 2:48640/76‑1 (MQ=255) aTACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAgcatgcat > 2:1808743/1‑76 (MQ=255) tACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCAtt < 1:5768075/76‑1 (MQ=255) tACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCAtt < 2:6347841/76‑1 (MQ=255) aaTTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCg > 1:1001943/1‑76 (MQ=255) aTTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCgg < 1:2104612/76‑1 (MQ=255) aTTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCgg > 1:1166282/1‑76 (MQ=255) aTTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCgg > 2:250096/1‑76 (MQ=255) tttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGa > 1:4340284/1‑76 (MQ=255) tttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGa > 1:1423228/1‑76 (MQ=255) ttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGAt < 2:6856713/76‑1 (MQ=255) ttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGAt > 2:3214404/1‑76 (MQ=255) ttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGAt < 1:2882266/76‑1 (MQ=255) ttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGAt < 1:1651051/76‑1 (MQ=255) gATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGc < 2:5268759/76‑1 (MQ=255) gATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGc > 2:1023153/1‑76 (MQ=255) gATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGc > 1:5589595/1‑76 (MQ=255) gATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGc > 1:3596060/1‑76 (MQ=255) aTGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCa < 1:6057156/76‑1 (MQ=255) ggTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCaaa < 1:5306661/76‑1 (MQ=255) gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAag < 1:2311298/76‑1 (MQ=255) gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAag > 1:3290609/1‑76 (MQ=255) gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAag < 1:235936/76‑1 (MQ=255) gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAag < 2:1430663/76‑1 (MQ=255) tGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAaga < 1:1937931/76‑1 (MQ=255) tGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAaga > 2:3689152/1‑76 (MQ=255) gCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAagag > 1:6415949/1‑76 (MQ=255) gCCCATCACGCACTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAagag > 1:856132/1‑76 (MQ=255) cccATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGc < 2:3576713/76‑1 (MQ=255) ccATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCTCCCCCAGTTTGCATTGCGGATGCAAAGAGCa < 2:4612952/76‑1 (MQ=255) ccATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCa < 2:2298505/76‑1 (MQ=255) ccATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCa < 2:6041762/76‑1 (MQ=255) ccATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCa > 2:2763742/1‑76 (MQ=255) cATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCaa > 2:6947918/1‑76 (MQ=255) cATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCaa > 2:1288603/1‑76 (MQ=255) cATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCaa < 2:5771617/76‑1 (MQ=255) tCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACg < 1:1796524/76‑1 (MQ=255) tCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACg < 1:5405264/76‑1 (MQ=255) aCGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGAt < 1:203585/76‑1 (MQ=255) cgcgCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATa > 1:6584167/1‑76 (MQ=255) gcgcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATaa < 1:4569165/76‑1 (MQ=255) gcgcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATaa > 1:2931618/1‑76 (MQ=255) cgcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAg > 1:705800/1‑76 (MQ=255) cgcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAg < 2:1052847/76‑1 (MQ=255) gcTGATTTTCGCCACTCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAgg < 2:2846534/76‑1 (MQ=255) gcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAgg > 2:2001703/1‑76 (MQ=255) gcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAgg > 1:2138870/1‑76 (MQ=255) gcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAgg > 1:2666542/1‑76 (MQ=255) gcTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAgg > 2:758490/1‑76 (MQ=255) cTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGt < 2:345168/76‑1 (MQ=255) cTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGt < 1:2443013/76‑1 (MQ=255) cTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGt < 2:5077101/76‑1 (MQ=255) cTGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGt > 1:5265935/1‑76 (MQ=255) cTGATTTTCGCCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGt < 2:4577710/76‑1 (MQ=255) tGATTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTa < 1:6600532/76‑1 (MQ=255) aTTTTCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACt < 1:409410/76‑1 (MQ=255) ttttCGCGACGCAACGAAATTACCAGCCTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACtt < 1:7365718/76‑1 (MQ=255) ttttCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACtt > 2:1599819/1‑76 (MQ=255) ttttCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACtt < 1:5875962/76‑1 (MQ=255) tttCGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTc > 2:7440215/1‑76 (MQ=255) cGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGtt < 1:3393222/76‑1 (MQ=255) cGCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGtt < 1:5776347/76‑1 (MQ=255) gCCACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTg < 1:2758685/76‑1 (MQ=255) ccACGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGc > 1:3929251/1‑76 (MQ=255) cGCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCAt > 1:1004180/1‑76 (MQ=255) gCAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATc < 2:3902450/76‑1 (MQ=255) cAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCa < 1:2849960/76‑1 (MQ=255) cAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCa < 1:315885/76‑1 (MQ=255) cAACGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCa > 1:5194834/1‑76 (MQ=255) aaCGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAg < 1:6935240/76‑1 (MQ=255) aaCGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAg > 1:2590938/1‑76 (MQ=255) aaCGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAg > 2:6087161/1‑76 (MQ=255) aaCGGAATTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAg > 2:120111/1‑76 (MQ=255) aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg < 2:2629693/76‑1 (MQ=255) aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg < 2:3643225/76‑1 (MQ=255) aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg > 2:2653786/1‑76 (MQ=255) aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg > 1:1490515/1‑76 (MQ=255) aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg > 2:1925880/1‑76 (MQ=255) aaTTACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg > 2:4160400/1‑76 (MQ=255) tcccAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGAcc < 1:6781603/74‑1 (MQ=255) tACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGAcc > 2:2033476/1‑76 (MQ=255) tACCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGAcc > 1:6117618/1‑76 (MQ=255) aCCAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCa > 1:4851873/1‑76 (MQ=255) ccAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCAc < 2:4892187/76‑1 (MQ=255) cAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACt < 1:5445608/76‑1 (MQ=255) cAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACt < 1:929203/76‑1 (MQ=255) cAGCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACt > 1:7376786/1‑76 (MQ=255) gCGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACTTc < 2:2687783/76‑1 (MQ=255) cGTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACTTCa < 1:1730642/76‑1 (MQ=255) gTCGCCCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACTTCAc < 2:4183053/76‑1 (MQ=255) | TTTTTGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACCACTTCACC > REL606/2164844‑2164989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |