| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,927,357 | (T)8→9 | coding (74/1266 nt) | rhlB ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,927,349 | 1 | . | T | 96.5% | 565.1 / 11.7 | 142 | coding (82/1266 nt) | rhlB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base T (47/89); total (49/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
Only 100 of 162 total aligned reads displayed.
CCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCC‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATG > REL606/3927274‑3927418 | ccTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAAccc > 2:4726524/1‑76 (MQ=255)ccTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAAccc > 2:1029955/1‑76 (MQ=255) gCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCC‑ttt > 1:826125/1‑76 (MQ=255) gCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCC‑ttt > 1:1690810/1‑76 (MQ=255) cgggCTACGCCCCGCCCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCC‑ttttt < 1:56693/74‑1 (MQ=255) ccGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCC‑ttttt > 2:14466/1‑76 (MQ=255) ggCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCC‑ttttttt > 1:2722596/1‑76 (MQ=255) gCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCC‑tttttttt > 2:6881423/1‑76 (MQ=255) gCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGGGCCTGAATGGGCGTACAGTTATG‑AAACCC‑tttttttt > 1:2268145/1‑76 (MQ=255) tCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAgg > 2:1572634/1‑76 (MQ=255) tCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAgg > 2:1749747/1‑76 (MQ=255) cACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAAACCT‑TTTTTTTTCAAggg > 1:1247065/1‑76 (MQ=255) ccccccGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCt < 2:4025700/73‑1 (MQ=255) cccccGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCtt < 2:2354830/74‑1 (MQ=255) cccccGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCta < 2:3971746/74‑2 (MQ=255) aCCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTc > 2:2143215/1‑76 (MQ=255) cccGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCt < 1:1064308/76‑1 (MQ=255) ccGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTa < 2:1900192/76‑1 (MQ=255) gCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACa < 2:5085468/76‑1 (MQ=255) gCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACa < 2:510326/76‑1 (MQ=255) ccAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACaa < 2:2262610/76‑1 (MQ=255) cAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAAc > 2:2638942/1‑76 (MQ=255) cAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAAc < 1:517334/76‑1 (MQ=255) cAGCGTCAGCGGAAGGCCCAGTGCCTGAATGGGCGTACAGTTATG‑AATCCCTTTTTTTTTCAAGGGCTTCTACAAc < 2:6154789/76‑1 (MQ=255) cGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAAACtt > 2:4145097/1‑76 (MQ=255) gTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTc > 2:4904631/1‑76 (MQ=255) gTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTc > 1:4101571/1‑76 (MQ=255) tCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCg < 1:1876138/76‑1 (MQ=255) tCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCg > 1:6423533/1‑76 (MQ=255) cAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCgg < 2:1012590/76‑1 (MQ=255) cAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCgg < 2:3825800/76‑1 (MQ=255) aGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGa < 1:5604075/76‑1 (MQ=255) gCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACACCCTTCGGAt < 2:5659088/76‑1 (MQ=255) gggaGGTCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGc < 2:1394253/73‑1 (MQ=255) ggAGGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGc < 1:2005197/76‑1 (MQ=255) ggAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGc < 2:4494453/76‑1 (MQ=255) gAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCa < 2:1191547/76‑1 (MQ=255) gAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCa > 1:5489984/1‑76 (MQ=255) gAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCa < 2:3299789/76‑1 (MQ=255) aGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAgg < 1:1885667/76‑1 (MQ=255) aGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAgg < 1:4575610/76‑1 (MQ=255) aGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAgg < 2:2691419/76‑1 (MQ=255) gggCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAggg < 2:888097/76‑1 (MQ=255) gggCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAggg < 1:1275123/76‑1 (MQ=255) cAGTGCCTGAATGGTCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGaa < 1:5073691/76‑1 (MQ=255) cAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGGGaa > 2:5553053/1‑76 (MQ=255) cAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGaa > 2:2888618/1‑76 (MQ=255) cAGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGaa < 2:2484588/76‑1 (MQ=255) aGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAg > 1:1734752/1‑76 (MQ=255) aGTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAg < 1:4732517/76‑1 (MQ=255) gTGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGt < 1:2693407/76‑1 (MQ=255) tGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTc > 2:6274746/1‑76 (MQ=255) tGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTc > 1:1516060/1‑76 (MQ=255) tGCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTc > 2:5466860/1‑76 (MQ=255) gCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCg > 1:2713994/1‑76 (MQ=255) gCCTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCg < 1:4168953/76‑1 (MQ=255) ccTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCgg < 2:3676847/76‑1 (MQ=255) ccTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCgg > 2:6258846/1‑76 (MQ=255) cTGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGa < 2:1286026/76‑1 (MQ=255) tGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaa < 2:6653896/76‑1 (MQ=255) tGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaa < 2:3438725/76‑1 (MQ=255) tGAATGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaa > 2:2078688/1‑76 (MQ=255) aaTGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaaaa < 2:275933/76‑1 (MQ=255) aaTGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaaaa < 1:2223642/76‑1 (MQ=255) aaTGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaaaa > 1:3559064/1‑76 (MQ=255) aTGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAAc > 1:5010223/1‑76 (MQ=255) tGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACt < 2:4673766/76‑1 (MQ=255) tGGGCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACt > 2:160501/1‑76 (MQ=255) ggCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttc < 2:4978776/76‑1 (MQ=255) gCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttct < 2:5991761/76‑1 (MQ=255) gCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttct < 2:4358048/76‑1 (MQ=255) gCGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttct < 1:6704039/76‑1 (MQ=255) cGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctg < 1:1132328/76‑1 (MQ=255) cGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctg < 2:2359255/76‑1 (MQ=255) cGTACAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctg > 2:1747090/1‑76 (MQ=255) aCAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttc > 2:3395256/1‑76 (MQ=255) cAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttct > 1:3067462/1‑76 (MQ=255) cAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttct < 2:26814/76‑1 (MQ=255) cAGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttct < 2:1943232/76‑1 (MQ=255) aGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttctg > 1:3679409/1‑76 (MQ=255) aGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttctg < 1:3525456/76‑1 (MQ=255) aGTTATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttctg < 1:6175280/76‑1 (MQ=255) ttATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTctgttctgtt > 1:6735496/1‑76 (MQ=255) ttATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTctgttctgtt < 2:3245579/76‑1 (MQ=255) ttATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTctgttctgtt > 2:6177573/1‑76 (MQ=255) ttATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTctgttctgtt > 1:6021642/1‑76 (MQ=255) ttATG‑AAACCC‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTa > 1:379397/1‑76 (MQ=255) tATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTa < 1:776003/76‑1 (MQ=255) tATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTa > 1:6019594/1‑76 (MQ=255) tATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTa < 2:4804169/76‑1 (MQ=255) tATG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTa > 2:4439175/1‑76 (MQ=255) aTG‑AACCCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTaa < 1:2101468/76‑1 (MQ=255) aTG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTaa < 1:4195170/76‑1 (MQ=255) aTG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTaa < 1:6515065/76‑1 (MQ=255) aTG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTaa < 2:733425/76‑1 (MQ=255) tG‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTaaa > 1:2556708/1‑76 (MQ=255) g‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAAt > 1:6227012/1‑76 (MQ=255) g‑AAACCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAAt < 2:3759323/76‑1 (MQ=255) aaaCCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAAtg < 1:5929398/76‑1 (MQ=255) aaaCCCTTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAAtg > 2:1955837/1‑76 (MQ=255) | CCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATG‑AAACCC‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATG > REL606/3927274‑3927418 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |