BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	1,306,055	Δ1,704 bp		[cls]–[kch]	[cls], [kch]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	1306055	1307758	1704	175 [0]	[7] 176	[cls]–[kch]	[cls],[kch]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 1306054	0 (0.000)	169 (0.780)	94/150	0.6	98.0%	coding (186/1461 nt)	cls	cardiolipin synthetase
*	?	REL606	1307759 =	7 (0.030)	169 (0.780)	94/150	0.6	98.0%	coding (105/1254 nt)	kch	voltage‑gated potassium channel

Only 100 of 176 total aligned reads displayed.

GGAGAGATCCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGC  >  REL606/1307684‑1307833

gctttaaggtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaa                                                                            <  2:4312950‑M2/1‑1 (MQ=255)

gctttaaggtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaa                                                                            >  1:1421537‑M2/76‑76 (MQ=255)

gctttaaggtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaa                                                                            <  2:5062367‑M2/1‑1 (MQ=255)

gctttaaggtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaa                                                                            >  1:6516033‑M2/76‑76 (MQ=255)

gctttaaggtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaa                                                                            <  2:2816295‑M2/1‑1 (MQ=255)

 ctttaaggtcgttaagccattttgcggtggaaggccacatcgcgctggcgcgctcagcgcggcgtttgcctaaaaa                                                                           <  2:6737704‑M2/2‑1 (MQ=255)

  tttaaggtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaaaa                                                                          >  2:2953492‑M2/74‑76 (MQ=255)

  tttaaggtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaaaa                                                                          <  1:2654676‑M2/3‑1 (MQ=255)

    taaggtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaaaaaa                                                                        >  1:612533‑M2/72‑76 (MQ=255)

    taaggtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaaaaaa                                                                        <  1:6706336‑M2/5‑1 (MQ=255)

     aaggtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAAt                                                                       >  1:1962032‑M2/71‑76 (MQ=255)

     aagggcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAAt                                                                       >  2:6401760‑M2/71‑76 (MQ=255)

       ggtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATaa                                                                     >  1:6522431‑M2/69‑76 (MQ=255)

        gtcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAg                                                                    <  2:3050586‑M2/9‑1 (MQ=255)

         tcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGc                                                                   <  2:2703862‑M2/10‑1 (MQ=255)

         tcgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGc                                                                   <  2:5015348‑M2/10‑1 (MQ=255)

          cgttaagccattttgcggtggaaggccacctcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCa                                                                  >  2:5911874‑M2/66‑76 (MQ=255)

          cgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCa                                                                  >  2:5452789‑M2/66‑76 (MQ=255)

          cgttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCa                                                                  >  2:5976200‑M2/66‑76 (MQ=255)

           gttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCaa                                                                 <  2:5243223‑M2/12‑1 (MQ=255)

            ttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAAt                                                                >  2:5894749‑M2/64‑76 (MQ=255)

            ttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAAt                                                                <  1:1087361‑M2/13‑1 (MQ=255)

            ttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAAt                                                                >  2:6543130‑M2/64‑76 (MQ=255)

            ttaagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAAt                                                                <  1:2366459‑M2/13‑1 (MQ=255)

              aagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATcc                                                              <  1:2757081‑M2/15‑1 (MQ=255)

              aagccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATcc                                                              >  2:2350977‑M2/62‑76 (MQ=255)

               agccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCa                                                             >  2:1021944‑M2/61‑76 (MQ=255)

                gccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCAt                                                            >  2:4349995‑M2/60‑76 (MQ=255)

                gccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCAt                                                            <  2:6467020‑M2/17‑1 (MQ=255)

                 ccattttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCAtt                                                           >  2:5729452‑M2/59‑76 (MQ=255)

                   attttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTa                                                         >  1:4508711‑M2/57‑76 (MQ=255)

                   attttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTa                                                         <  1:2190982‑M2/20‑1 (MQ=255)

                   attttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTa                                                         >  2:2064254‑M2/57‑76 (MQ=255)

                   attttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTa                                                         <  1:611889‑M2/20‑1 (MQ=255)

                    ttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTaa                                                        <  1:383957‑M2/21‑1 (MQ=255)

                     tttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTaaa                                                       >  2:3635913‑M2/55‑76 (MQ=255)

                       tgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTaaaaa                                                     >  1:2266094‑M2/53‑76 (MQ=255)

                       tgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTaaaaa                                                     >  1:1371432‑M2/53‑76 (MQ=255)

                        tttGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAg                                                    >  1:946362/1‑76 (MQ=255)

                        gcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAg                                                    <  2:1017057‑M2/25‑1 (MQ=255)

                        gcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAg                                                    <  1:738152‑M2/25‑1 (MQ=255)

                         cggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGa                                                   >  2:6293436‑M2/51‑76 (MQ=255)

                            tggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACgg                                                >  1:171689‑M2/48‑76 (MQ=255)

                             tatGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGc                                               <  1:6908286/76‑1 (MQ=255)

                             ggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGc                                               <  1:2803150‑M2/30‑1 (MQ=255)

                             ggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGc                                               >  2:4158472‑M2/47‑76 (MQ=255)

                             ggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGc                                               >  2:5167543‑M2/47‑76 (MQ=255)

                               tGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCa                                             <  1:2841747/76‑1 (MQ=255)

                               aaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCa                                             >  2:4820767‑M2/45‑76 (MQ=255)

                               aaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCa                                             <  1:3690440‑M2/32‑1 (MQ=255)

                               aaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCa                                             >  2:1227655‑M2/45‑76 (MQ=255)

                                aggccgcatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAg                                            <  1:914687‑M2/33‑1 (MQ=255)

                                aggccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAg                                            >  1:3064598‑M2/44‑76 (MQ=255)

                                aaggccccatcgctctggcgcgcccagcgcggcgtttgcctaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAg                                            <  2:6790344‑M2/33‑1 (MQ=255)

                                  gccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAgcg                                          <  1:3938841‑M2/35‑1 (MQ=255)

                                  gccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAgcg                                          >  2:6209372‑M2/42‑76 (MQ=255)

                                   ccacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAgcgc                                         >  1:5620438‑M2/41‑76 (MQ=255)

                                    cacatcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGcgcg                                        >  1:3802468‑M2/40‑76 (MQ=255)

                                      catcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCgag                                      <  1:6610832‑M2/39‑1 (MQ=255)

                                       atcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCgaga                                     >  2:3393772‑M2/37‑76 (MQ=255)

                                        tcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGaa                                    <  1:6798660‑M2/41‑1 (MQ=255)

                                        tcgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGaa                                    >  1:1595320‑M2/36‑76 (MQ=255)

                                         cgctctggcgcgctcagcgcggcgtttgcctaaaAAAAGTAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAAt                                   <  2:3726770‑M2/42‑1 (MQ=255)

                                         cgctctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAAt                                   <  1:3376233‑M2/42‑1 (MQ=255)

                                            tctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTc                                >  2:820014‑M2/32‑76 (MQ=255)

                                            tctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTc                                >  2:5930981‑M2/32‑76 (MQ=255)

                                             ctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCg                               <  1:4593710‑M2/46‑1 (MQ=255)

                                             ctggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCg                               >  2:1404843‑M2/31‑76 (MQ=255)

                                              tggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGt                              >  2:6424421‑M2/30‑76 (MQ=255)

                                              tggcgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGt                              <  1:5382790‑M2/47‑1 (MQ=255)

                                                 cgcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGcc                           >  1:2551850‑M2/27‑76 (MQ=255)

                                                  gcgctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCg                          <  2:2276880‑M2/51‑1 (MQ=255)

                                                    gctcagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGaa                        <  2:130932‑M2/53‑1 (MQ=255)

                                                       cagcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAggg                     >  1:617455‑M2/21‑76 (MQ=255)

                                                        agcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGt                    >  2:4763830‑M2/20‑76 (MQ=255)

                                                        agcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGt                    <  2:1861743‑M2/57‑1 (MQ=255)

                                                        agcgcggcgtttgccaaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGt                    >  1:1024845‑M2/20‑76 (MQ=255)

                                                         gcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGATGGCCAGCGCGAGAATGTCGTGCCGAAGGGTc                   <  2:5380159‑M2/58‑1 (MQ=255)

                                                         gcgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTc                   <  1:534798‑M2/58‑1 (MQ=255)

                                                          cgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCa                  >  1:2534959‑M2/18‑76 (MQ=255)

                                                          cgcggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCa                  >  2:5805651‑M2/18‑76 (MQ=255)

                                                             ggcgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCAccc               >  1:802083‑M2/15‑76 (MQ=255)

                                                               cgtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCAt             <  2:4566673‑M2/64‑1 (MQ=255)

                                                                tAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATa            <  2:3491159/76‑1 (MQ=255)

                                                                gtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATa            <  1:577329‑M2/65‑1 (MQ=255)

                                                                gtttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATa            >  2:1999400‑M2/12‑76 (MQ=255)

                                                                  ttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATaaa          <  2:2763077‑M2/67‑1 (MQ=255)

                                                                  ttgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATaaa          >  2:3220284‑M2/10‑76 (MQ=255)

                                                                   tgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAAt         >  2:5209655‑M2/9‑76 (MQ=255)

                                                                   tgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAAt         >  1:5587224‑M2/9‑76 (MQ=255)

                                                                   tgcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAAt         >  1:4919728‑M2/9‑76 (MQ=255)

                                                                   tgccgaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAAt         <  1:2040240‑M2/68‑1 (MQ=255)

                                                                    gcctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAAtt        >  1:3667301‑M2/8‑76 (MQ=255)

                                                                     cctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAAttt       >  2:3936834‑M2/7‑76 (MQ=255)

                                                                     cctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAAttt       >  2:1666311‑M2/7‑76 (MQ=255)

                                                                     cctaaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGCCACCCATAAAttt       <  2:964121‑M2/70‑1 (MQ=255)

                                                                       taaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATttgt     >  1:4738115‑M2/5‑76 (MQ=255)

                                                                       taaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATttgt     >  2:4856128‑M2/5‑76 (MQ=255)

                                                                        aaaAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATttgtt    >  1:2893597‑M2/4‑76 (MQ=255)

                                                                          aAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGc  <  2:938952‑M2/75‑1 (MQ=255)

GGAGAGATCCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGC  >  REL606/1307684‑1307833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`