| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,685,638 | 0 | T | G | 33.6% | 235.4 / 38.1 | 110 | V326G (GTG→GGG) | ygaF | predicted enzyme |
| Reads supporting (aligned to +/- strand): ref base T (7/66); new base G (37/0); total (44/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.48e-22 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 156 total aligned reads displayed.
TGCTGCAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATC > REL606/2685563‑2685713 | tgctgcAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGt < 1:2811882/76‑1 (MQ=255) gctgcAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTg < 1:340068/76‑1 (MQ=255) tgcAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCa < 2:934535/76‑1 (MQ=255) tgcAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCa < 1:4397613/76‑1 (MQ=255) tgcAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCa < 1:1813944/76‑1 (MQ=255) tgcAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCa < 1:638766/76‑1 (MQ=255) gcAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCaa < 2:5292651/76‑1 (MQ=255) cAAATCCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCaaa < 2:1282640/76‑1 (MQ=255) cAAAACCATCTCCGCTAGGGCCGGGGGGAGATGAAAAACTCGCTGGGCAAAAGCGGCTATCTGCGGCT‑GTGCaaaa < 2:5473991/76‑1 (MQ=255) aaaaCCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCaaaa > 2:2027925/1‑76 (MQ=255) aaaCCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCGAAAg > 2:4914849/1‑76 (MQ=255) aaCCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGt < 2:4467770/76‑1 (MQ=255) aCCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTa < 2:6749361/76‑1 (MQ=255) aTCTACGCTCAGGACTGGGCGAGATGAAGAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTg < 2:50681/76‑1 (MQ=255) aTCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTg < 2:793321/76‑1 (MQ=255) tCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGt < 2:167835/76‑1 (MQ=255) tCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGt < 2:5238985/76‑1 (MQ=255) tCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGAATTGt > 2:6489130/1‑76 (MQ=255) cTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGGCAAAGTATTGTc > 1:4685424/1‑76 (MQ=255) cTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGGAAAAGTATTTTc > 1:3647604/1‑76 (MQ=255) cTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCCAAAACATTGtt > 2:5664559/1‑75 (MQ=255) tACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTcc < 2:640442/76‑1 (MQ=255) aCGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAAAATTGTtcc > 1:4903731/1‑76 (MQ=255) cGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTcccc < 2:2617005/76‑1 (MQ=255) gCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCg < 1:1258794/76‑1 (MQ=255) gCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCg < 1:948069/76‑1 (MQ=255) cTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCCAAAGTATTTTcccccg > 2:2770737/1‑74 (MQ=255) cTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCgg < 2:5002863/76‑1 (MQ=255) cTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCgg < 2:6893585/76‑1 (MQ=255) cTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGTATTTTCCCCgg > 1:1926647/1‑76 (MQ=255) tCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGGAAAAGAATTTTTCCCCGc > 1:1270796/1‑76 (MQ=255) tCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGGAAAAATATTTACCCCgcc > 2:5536210/1‑74 (MQ=255) cAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCt < 1:6606645/76‑1 (MQ=255) aGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCtt < 1:4949112/76‑1 (MQ=255) aGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCtt < 2:4560930/76‑1 (MQ=255) aGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCtt < 1:3364701/76‑1 (MQ=255) ggACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCttt < 1:688179/76‑1 (MQ=255) ggACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCGAAAGTATTGTCCCCGGCctt > 2:4039467/1‑76 (MQ=255) gACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTc < 2:1397509/76‑1 (MQ=255) gACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTc < 2:5367875/76‑1 (MQ=255) gACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGTATTGTCCCCGGCTTTc > 1:5216499/1‑76 (MQ=255) cTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGGAAAAGGATTTTCCCCGGCTTTCGt > 1:869306/1‑76 (MQ=255) tGGGGGAGATGAAAAACTCGCTGTGCTAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGtt < 2:6589538/76‑1 (MQ=255) tGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGtt < 2:1076522/76‑1 (MQ=255) tGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGtt < 1:2959356/76‑1 (MQ=255) tGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAAAATTTTTCCCCGCTTTCGtt > 1:5987801/1‑76 (MQ=255) gggCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTa < 2:4790418/76‑1 (MQ=255) gggCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGGAAAAAAATTGTCCCCCGCGTTCGTTa > 1:1399156/1‑76 (MQ=255) gggCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGTATTGTCCCCCGCGTTCGTTa > 2:6764141/1‑76 (MQ=255) ggCTAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTaa < 1:5814950/76‑1 (MQ=255) ggCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTaa < 1:6019129/76‑1 (MQ=255) ggCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTaa < 1:3657735/76‑1 (MQ=255) ggCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAAAATTTTTCCCCGCCTTTGTTaa > 1:4099956/1‑76 (MQ=255) gCGAGATGAAAACCTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAg < 1:6262994/76‑1 (MQ=255) gCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAg < 1:2843408/76‑1 (MQ=255) gCGAGATGAAAAACTCACTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAg < 2:3316016/76‑1 (MQ=255) agaTGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGGCAAAGTATTTTTCCCGGCTTTCGTTAAGCGa > 1:4807469/1‑76 (MQ=255) agaTGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGGAAAAGTATTGTCCCCGGCTTTCGTTAAGCGa > 2:4348934/1‑76 (MQ=255) agaTGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGTGTTGTCCCCGGCTTTCGTTAAGGGa > 1:6607969/1‑76 (MQ=255) gaTGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGGAAAAAGATTGTCCCCGGCTTTCGTTTAGCGac > 1:1368010/1‑75 (MQ=255) gacaaaCTCGCTGGGCAAAACGGGCTATCGGCGGCTGGTGCAAGAGGATTGTCCCCGGCTTGCGGTAAGCGAcc‑tc < 2:3861391/73‑1 (MQ=255) gAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGGATTTTTCCCCGCTTTTGTTAAGAGGtc‑tc > 2:3921776/1‑76 (MQ=255) aaaaCTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCa < 2:663021/76‑1 (MQ=255) aaaaCTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCa < 1:1803494/76‑1 (MQ=255) aaaaCTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGGCCAAGAATTATTCCCCGCTTTCGTTAAACGACC‑Tccc > 1:4427125/1‑75 (MQ=255) aaaaCTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGGATTGTCCCCCGCTTTTGTTAAGCGATC‑Tccc > 2:4827557/1‑75 (MQ=255) aCTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGGCAAAGGATTGTCCCCGGGCTTTGTTAAACGATG‑TCCCGcc > 2:1413037/1‑76 (MQ=255) tCGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAACCCt > 1:3197656/1‑76 (MQ=255) cGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTg < 1:4081567/76‑1 (MQ=255) cGCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAcccccg > 1:4333150/1‑74 (MQ=255) gCTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGTATTTTCCCCCGCTTTCGTTTAACGATC‑TCCACCCCCgg > 1:701170/1‑76 (MQ=255) cTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCCGGc > 1:5969128/1‑76 (MQ=255) cTGTGCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGGATTGTCCCCGGCTTTCGTTAAGCGATA‑TCCAGCCCTGGc > 1:713849/1‑76 (MQ=255) tgtgCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGcc > 1:6485505/1‑76 (MQ=255) tgtgCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGGATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGcc > 1:1860164/1‑76 (MQ=255) tgCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCCGCCCTGGcccc > 1:1084178/1‑75 (MQ=255) gCAAAAGCGGCTATCTGCGGCTGGGGCAAAAGTATTGTCCCCCGCTTTCGTTTAGCGATC‑TCCACCCCTTGccccc > 1:2356472/1‑74 (MQ=255) aaaaGCGGCTATCTGCGGCTGGGGGAAAAAGATTTTCCCCCGCCTTTCTTAAACGGTCTTCCGGCCCGGGcccccg > 2:2970843/1‑73 (MQ=255) aaaGCGGCTATCTGCGGCTGGGGGAAAAAAATTGTCCCCGGCGTTTGTTAAGCGGAC‑TCCAACCCCGGCccgcccg > 2:2297354/1‑74 (MQ=255) aaGCGGCTATCTGCGGCTGGGGGCAAAGGATTGGCCCCCGCCTTCGTTAAGCGGGC‑TCCAGCCCCGGgccgcccgg > 2:23061/1‑73 (MQ=255) aaGCGGCTATCTGCGGCTGGGGCAAAAATATTGGCCCCGGCTTTCGTTTATCGATC‑TCCAGCCCTGGGccccccgc > 1:1009682/1‑73 (MQ=255) aaGCGGCTATCTGCGGCTGGGGCAAAAAAATTGTCCCCGGGTTTCGTTAAGCGATC‑TCCAGCCCCGGCCCGCCCgt > 2:4697407/1‑76 (MQ=255) aaGCGGCTATCGGCGCTGGGTGAAAAAGTTTTCCCCCCGCCTTTCGTTAAGCGATC‑CCCCGCCCGGCCCCGCCGgt < 2:4540578/76‑1 (MQ=255) cGGCTGTCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑CCCAGCCCGGGCCCGCCGGtgtg < 2:2635401/76‑1 (MQ=255) cTATCTGCGGCTGGGGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCGGTGTGCgg > 1:3068266/1‑76 (MQ=255) aTCTGCGGCTGGGGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCCGTGGGCgggg > 1:5205254/1‑75 (MQ=255) aTCTGCGGCTGGGGCAAAAGAATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCGGTGTGCgggg > 2:6782016/1‑75 (MQ=255) cTGCGGCTGGTGCAAAAGGATTGTCCCCCGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCCGTGTGCGGgcgc > 2:4827799/1‑76 (MQ=255) cTGCGGCTGGGGCAAAAGTATTGTCCCCGGCTTTCGTTAAGAGATC‑TCCACCCCTCGCCCCCCGGGGTGTGGgcgc > 2:1588984/1‑76 (MQ=255) gCGGCTGGGGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCCGGCCCGCCGGTGTGCGGGGGCAg > 2:6673861/1‑76 (MQ=255) cGGCTGTTGAAAAATTATTGCCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAgg < 2:5266930/76‑1 (MQ=255) cGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCGGTGTGCGGGGGCAgg > 2:1936740/1‑76 (MQ=255) cGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑CCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAgg < 1:2432761/76‑1 (MQ=255) gCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCGGTGTGCTGGGGCTGGCg > 2:3020879/1‑76 (MQ=255) cTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCgg > 2:3200289/1‑76 (MQ=255) tGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCGGCGTACGGGCGCAGGCggg > 2:359449/1‑75 (MQ=255) gggTGGAATAGTTGTGCCCCGGGCTTCAGTAAAGACATC‑CCCACCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGt < 2:1093870/75‑1 (MQ=255) gTGCAAAAGTTTTGCCCCCGGCTTTCTTTAAGCGATC‑TCCACCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTAt < 1:1980756/76‑1 (MQ=255) tGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATc > 2:742575/1‑76 (MQ=255) tGCAAAAGTATTCCCCCCGGCTTTCGTAAAGCGATC‑CCCAGCCCGGCCCCGCCGGTGTGCGGGCGCAGGCGGTATc < 2:4867887/76‑1 (MQ=255) | TGCTGCAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATC‑TCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATC > REL606/2685563‑2685713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |