| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,378,885 | 0 | T | G | 36.6% | 463.2 / 66.0 | 224 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
| Reads supporting (aligned to +/- strand): ref base T (25/117); new base G (82/0); total (107/117) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-39 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 270 total aligned reads displayed.
GCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378810‑3378960 | gCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgt < 1:6677381/76‑1 (MQ=255)gCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgt < 1:5017138/76‑1 (MQ=255)gCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgt < 1:4633883/76‑1 (MQ=255) cTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgtg < 2:6909020/76‑1 (MQ=255) cTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgtg < 2:6381815/76‑1 (MQ=255) tGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgtgt < 1:5329815/76‑1 (MQ=255) tGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgtgt > 2:4436337/1‑76 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGtt < 1:1982882/76‑1 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGtt > 1:1199838/1‑76 (MQ=255) agcGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTa < 2:6742516/74‑1 (MQ=255) aCCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTa < 2:1799631/76‑1 (MQ=255) aCCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTa < 1:6480390/76‑1 (MQ=255) aCCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTa < 2:3763696/76‑1 (MQ=255) aCCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTa < 1:1822937/76‑1 (MQ=255) ccGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTAc < 2:1939918/76‑1 (MQ=255) cGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTAcc > 2:1229342/1‑76 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTAACTgg > 2:5919710/1‑76 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGt < 1:3091255/76‑1 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTggg > 1:4035530/1‑75 (MQ=255) ttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGtt < 1:540876/76‑1 (MQ=255) ttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGtt < 1:4901448/76‑1 (MQ=255) tACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGttt > 1:4248394/1‑76 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTg < 1:641212/76‑1 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCCGGGTTg > 2:4347620/1‑76 (MQ=255) aCCTGGTTTCACTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTg > 2:4734940/1‑76 (MQ=255) ccTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGa < 2:5489267/76‑1 (MQ=255) cTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGAt > 2:2963181/1‑76 (MQ=255) cTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTAACTGGGTTGAt > 2:5313050/1‑76 (MQ=255) tGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGAtt < 1:1562681/76‑1 (MQ=255) tGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGAtt < 2:4066095/76‑1 (MQ=255) tGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGAtt < 1:5159886/76‑1 (MQ=255) tGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGGTTGAtt > 1:4398627/1‑76 (MQ=255) gTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTTATTgc > 2:1461425/1‑76 (MQ=255) gTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGGTTTATTgc > 2:2190596/1‑76 (MQ=255) tttCATTTCTCCGTTATGAACACTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgca < 1:480591/76‑1 (MQ=255) tttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTgca > 2:2607628/1‑76 (MQ=255) ttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcag < 1:1386003/76‑1 (MQ=255) ttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcag < 2:865102/76‑1 (MQ=255) tttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATc > 2:6143193/1‑76 (MQ=255) ttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTCCTGGGTTGATTGCAGCATCa > 1:3294242/1‑76 (MQ=255) ttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCa > 1:6801835/1‑76 (MQ=255) ttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGTTTGATTGCAGCATCa > 1:4772956/1‑76 (MQ=255) tCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCaa < 1:3609864/76‑1 (MQ=255) tCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCaa < 1:6272981/76‑1 (MQ=255) tCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTACTGGGTTGATTGCAGCATCaa > 1:2107154/1‑76 (MQ=255) tCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGTTTGCAGCATTaa > 1:1608861/1‑76 (MQ=255) tCTACGTTATGAACAGTAGAACCAACCGGGATGCTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGTAGCATCaa > 2:4546970/1‑76 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTCCTGGTTTGATTGCAGCATCaaa > 1:2879199/1‑75 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGTTTGATTGCCGCATCAAc > 1:1464256/1‑76 (MQ=255) tACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACg < 2:6413535/76‑1 (MQ=255) tACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACg > 2:1371868/1‑76 (MQ=255) aCGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTAGCTGGTTTGATTGCAGGATCAACGc > 2:3613480/1‑76 (MQ=255) cGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGcc > 2:4999744/1‑76 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAg < 1:1133781/76‑1 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAg < 1:998089/76‑1 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAg < 1:5678539/76‑1 (MQ=255) tATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGa > 2:1091301/1‑76 (MQ=255) tGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACt < 1:247682/76‑1 (MQ=255) tGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACt < 1:2596828/76‑1 (MQ=255) gAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTg < 2:6862220/76‑1 (MQ=255) aaCAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGGTTCCTGGCTTGATTGAAGAATCAACGCCCCACTGa > 2:2853080/1‑76 (MQ=255) aaCAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGGTTGATTGGAGGATCAACGCCCGACTGa > 1:3018440/1‑76 (MQ=255) cAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAAt < 1:1874977/76‑1 (MQ=255) cAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAAt > 2:5349257/1‑76 (MQ=255) cAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATCAACGCCCGACTGAAt > 2:2442244/1‑76 (MQ=255) gTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCt < 2:4891089/76‑1 (MQ=255) gTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCCGAATGAATCt > 1:2895911/1‑76 (MQ=255) tAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGAACGAATccg > 1:5812447/1‑74 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTgg < 1:1042129/76‑1 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTgg < 2:21526/76‑1 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGcc < 1:2171052/76‑1 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGcc < 1:163056/76‑1 (MQ=255) accGGGATGTTGCGCATCGGCATGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAg < 2:1716294/76‑1 (MQ=255) accGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAg < 1:6079547/76‑1 (MQ=255) cGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGGAGCATCAACGCCAGACTGAATCTGGTCGCCAGCt > 1:6027983/1‑76 (MQ=255) cGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCt < 1:971033/76‑1 (MQ=255) cGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCt < 2:5602175/76‑1 (MQ=255) gggATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCtt < 1:3911246/76‑1 (MQ=255) gggATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCCGACTGAATCTGGTCGCCCGCtt > 1:4363653/1‑76 (MQ=255) gggATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATCAAAGCCCGACTGAATCTGGTCGCCAGCtt > 2:2995276/1‑76 (MQ=255) gATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTc < 1:5465525/76‑1 (MQ=255) aTGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCCGACTGAATCTGGTCGCCAGCCTTCa > 2:3998208/1‑76 (MQ=255) aTGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCa > 2:5863826/1‑76 (MQ=255) aTGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCa > 2:6228307/1‑76 (MQ=255) gTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAgg < 2:3855948/76‑1 (MQ=255) gTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAgg < 2:3182268/76‑1 (MQ=255) tGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGcc > 2:6102289/1‑76 (MQ=255) gcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCttt < 2:4798983/76‑1 (MQ=255) cATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTa < 2:6614532/76‑1 (MQ=255) cATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTa < 2:4227015/76‑1 (MQ=255) aTCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAg < 1:2693936/76‑1 (MQ=255) aTCGGCAGGGGGGTTTCTGGTTTTATTGCAGCATCAACGCCAGACTGAAACTGGTCGCCCGCCTTCACGCCttttg > 2:76151/1‑74 (MQ=255) cGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAggg > 1:3937115/1‑76 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 1:5520892/1‑76 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc < 1:6867972/76‑1 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 1:769772/1‑76 (MQ=255) aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa > 2:1023356/1‑76 (MQ=255) ggTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAgg > 1:865564/1‑76 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt < 2:6508132/76‑1 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt > 1:3162830/1‑76 (MQ=255) | GCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378810‑3378960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |