| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,595,070 | 0 | A | G | 33.6% | 278.0 / 80.6 | 149 | G11G (GGA→GGG) | yjjZ | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (15/84); new base G (50/0); total (65/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-26 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 216 total aligned reads displayed.
TCATATGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATT > REL606/4594995‑4595145 | cATATGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAg < 1:2235372/76‑1 (MQ=255) atatGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAgt < 2:6064194/76‑1 (MQ=255) atatGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAgt < 1:6716800/76‑1 (MQ=255) atatGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAgg > 2:3959439/1‑75 (MQ=255) tGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGtt > 1:5527213/1‑76 (MQ=255) tGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGtt < 2:2005605/76‑1 (MQ=255) gATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTtg > 1:3499745/1‑76 (MQ=255) gATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTtg < 1:3839675/76‑1 (MQ=255) gATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTtg < 1:2640104/76‑1 (MQ=255) atatTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTtgc > 1:6809405/1‑76 (MQ=255) atatTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGGGGtgc > 2:4814631/1‑76 (MQ=255) tatTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGGtgct > 2:3566048/1‑76 (MQ=255) atTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTtggtg > 2:2372912/1‑76 (MQ=255) atTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTtgctg > 2:1217058/1‑76 (MQ=255) atTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTtgctg < 1:6040361/76‑1 (MQ=255) tGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGGTGCTGcc > 1:2769456/1‑76 (MQ=255) gTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGcccg > 2:1118744/1‑74 (MQ=255) gTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGGCgg > 2:4270100/1‑76 (MQ=255) gTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCgg > 2:3146503/1‑76 (MQ=255) gTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCgg < 1:502359/76‑1 (MQ=255) gTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCgg < 1:4429366/76‑1 (MQ=255) gTTATCATTATCAATGAGAGAGAAGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCgg < 2:3406694/76‑1 (MQ=255) ttatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGTGTTGCCGCCggg > 1:4751836/1‑76 (MQ=255) ttatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCcgg > 1:1246073/1‑76 (MQ=255) ttatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGGTGCTGCCggg > 1:2155597/1‑76 (MQ=255) ttatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCggg < 1:3212266/76‑1 (MQ=255) ttatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCcgg > 2:633242/1‑76 (MQ=255) tatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGGCgggg > 1:2617109/1‑75 (MQ=255) tatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCgggg > 1:5319427/1‑75 (MQ=255) tatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGa < 2:4493609/76‑1 (MQ=255) atcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGGTGCTggggt > 1:6480216/1‑74 (MQ=255) atcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGAt < 2:128758/76‑1 (MQ=255) atcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGAt < 2:6416658/76‑1 (MQ=255) atcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGGGTTGCTGCCggggt > 1:6491763/1‑74 (MQ=255) tcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGTGGTGCTGGCgggggt > 2:258397/1‑73 (MQ=255) tcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGAtt < 2:1604477/76‑1 (MQ=255) cattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGGTTg > 2:4359127/1‑76 (MQ=255) cattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGGTGCTGCCGGGGTTg > 1:1846292/1‑76 (MQ=255) ttatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCCGGGTTGCt > 2:474262/1‑76 (MQ=255) ttatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGGTGCTGCCGGGGTTGCt > 1:1974211/1‑76 (MQ=255) tatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGTGTTGCTGGCGGGGTTGCTg > 2:6206953/1‑76 (MQ=255) tatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTg < 2:6941894/76‑1 (MQ=255) atcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCCGGGGTGCTGa > 1:2419769/1‑76 (MQ=255) atcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCCGGATTGCTgt > 2:6914156/1‑75 (MQ=255) tcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGAt < 2:1063189/76‑1 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGTGTTGCTGCCGGGGTTGCTGAtt > 2:3264037/1‑76 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGGTGCCGGGGTTGCTGGtt > 1:4239250/1‑76 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGGTTGCTGAtt > 2:1026633/1‑76 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGGGTGCTGAtt > 2:2488684/1‑76 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGGTGCTGCCGGGGTTGCTGGtt > 1:3177500/1‑76 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGCGGTGCTGCCGGGAATGCCGAtt > 2:2245413/1‑76 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGAtt < 1:6799769/76‑1 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGAtt < 1:6388776/76‑1 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGGGTTGGTGGCGGGCGTGCTGAtt > 2:5084666/1‑76 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGGGGTGCTGCCGGGGTTGCTGAtt > 1:1572224/1‑76 (MQ=255) aTGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGGTTGGTGATTgg > 1:3542722/1‑75 (MQ=255) aTGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGGGTGCTGGTTGc > 1:299070/1‑76 (MQ=255) tGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGAGTGCTGATTGcg > 2:4860067/1‑75 (MQ=255) gagagagaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAg < 2:6151349/76‑1 (MQ=255) gagagagaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAg < 1:2351289/76‑1 (MQ=255) agagagaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGGGTTGCTGCCGGGGTTGCTGATTGGAgg > 1:1759372/1‑76 (MQ=255) gagagaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAggg < 1:4409012/76‑1 (MQ=255) gagagaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAggg < 2:5419985/76‑1 (MQ=255) agaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGATTGCTGATTGCAGGGGTg > 1:1281103/1‑76 (MQ=255) agaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGAGTGCTGATTGCAggggcg > 1:6657118/1‑73 (MQ=255) aaaTCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGGGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGtt > 2:16248/1‑75 (MQ=255) tCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGGGGTGGTGCCGGGGTTGGTGATTGCAGGGGTGGTGTATgg > 1:3688706/1‑75 (MQ=255) cATGTTGCAACGTACGCTGGGCAGTGGCTGGGGGGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGGTGTATggg > 2:6683815/1‑74 (MQ=255) aTGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCgg < 1:6164104/76‑1 (MQ=255) tGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGAttt < 1:3362079/76‑1 (MQ=255) aaCGTACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGGTTGCTGATTGCCGGGCTGATGTATGCGGGTTTTTc > 2:1543175/1‑76 (MQ=255) aaCGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATc < 1:2082095/76‑1 (MQ=255) aCGTACGCTGGGCAGTGGCTGGGGGGTGGTGCTGCCCGGGTTGCTGATTGCAGGGGTGATGTATGCGGGTTTATCg > 2:2861651/1‑76 (MQ=255) cGTACGCTGGGCAGTGGCTGGGGGGTGTTGCTGCCGGGGTTGCTGATTGCAGGGGTGATGTATGCGGGATTATCGt > 2:5786788/1‑76 (MQ=255) gTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTc < 1:1135755/76‑1 (MQ=255) gTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTc < 1:1347613/76‑1 (MQ=255) tACGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGGGTGCTGATTGCAGGGGCGATGTATGCGGGTTTTTCGTCa > 2:842440/1‑76 (MQ=255) aCGCTGGGCAGTGGCTGGGGGGTGTTGCTGCCGGGATTGCTGATTGGAGGGGTGGTTTATGAGGGTTTATCGTCAg > 2:5568102/1‑76 (MQ=255) aCGCTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGGTTGCTGATTGCCGGGCTGATGTATGCGGATTTATCGTCAg > 2:497204/1‑76 (MQ=255) gCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGAt < 2:2300698/76‑1 (MQ=255) gCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGAt < 2:5725731/76‑1 (MQ=255) cTGGGCAGTGGCTGGGGGGGGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATc > 2:1772459/1‑76 (MQ=255) cTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATc < 2:3071784/76‑1 (MQ=255) cTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATc < 1:5236484/76‑1 (MQ=255) gggCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAg < 1:4225733/76‑1 (MQ=255) cAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTgg < 1:4859062/76‑1 (MQ=255) aGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGc < 2:3811196/76‑1 (MQ=255) aGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGc < 1:4361609/76‑1 (MQ=255) tGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCgg < 2:780293/76‑1 (MQ=255) ggCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCggg > 2:5906309/1‑75 (MQ=255) ggCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGa > 1:2123089/1‑76 (MQ=255) gCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGAt < 1:4093876/76‑1 (MQ=255) cTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGAtt > 1:2830654/1‑76 (MQ=255) tGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTg > 2:5674718/1‑76 (MQ=255) gggAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTc > 1:4604702/1‑76 (MQ=255) ggAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCa > 2:6538304/1‑76 (MQ=255) ggAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCa > 2:3303847/1‑76 (MQ=255) gAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCAt < 2:1824650/76‑1 (MQ=255) aGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCAtt < 2:1461917/76‑1 (MQ=255) aGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCAtt < 2:2100064/76‑1 (MQ=255) | TCATATGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATT > REL606/4594995‑4595145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |