| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 720,464 | 0 | C | G | 50.0% | 3.0 / 23.9 | 20 | A22G (GCC→GGC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base C (3/7); new base G (10/0); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGGAGTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGCCGCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATAACAT > REL606/720389‑720539 | tCAGGAGTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctgc > 1:2654794/1‑76 (MQ=255) aGGAGTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctgccg < 2:917135/76‑1 (MQ=255) gAGTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGTATTTACGTCTGCACGATAATCTCGCACTGGctgccgcc < 2:6696229/76‑1 (MQ=255) ggTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCTCTGGctgccgcc‑t < 2:5202924/75‑1 (MQ=255) aGTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggcgccc > 1:5530544/1‑75 (MQ=255) aGTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggcgccc > 1:6580604/1‑75 (MQ=255) aGTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggcgccc > 1:4787710/1‑75 (MQ=255) aGTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctgccgcc‑t < 2:2820701/76‑1 (MQ=255) aGTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctgccgcc‑t < 1:2071132/76‑1 (MQ=255) gTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggcgcccc > 1:5251492/1‑74 (MQ=255) gTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctgccgcc‑tg < 1:2485821/76‑1 (MQ=255) gTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctgccgcc‑tg < 2:3725001/76‑1 (MQ=255) gTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctgccgcc‑tg < 1:2871223/76‑1 (MQ=255) ttttATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggcgccccc > 1:3898656/1‑73 (MQ=255) ttttATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggcgcc‑cgc > 1:3894024/1‑76 (MQ=255) tttATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggggcc‑cgcc > 2:5446144/1‑76 (MQ=255) ttATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggcgcc‑cgccc > 1:3664136/1‑75 (MQ=255) tATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggggcc‑cgccgc > 2:3124286/1‑76 (MQ=255) aTGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggcgcc‑cgccggc > 1:4235772/1‑74 (MQ=255) aTGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggcgcc‑cgccgcc > 2:2797818/1‑75 (MQ=255) tGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGGC‑CCCCGCac > 2:1849788/1‑75 (MQ=255) gATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGctggcgcc‑cgccgccca > 1:4220675/1‑73 (MQ=255) gATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCGCCAt > 1:3632373/1‑76 (MQ=255) ttACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCGCCATTc > 1:6078683/1‑76 (MQ=255) tACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGGC‑TGCCGCCATTcc > 2:1705253/1‑75 (MQ=255) tACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCCCCATTCg > 1:1862012/1‑76 (MQ=255) aCCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCGCCACTCGt > 1:5851033/1‑76 (MQ=255) aCCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCCCC‑GGCCACACTTTGt > 1:4811075/1‑76 (MQ=255) cATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGGGGC‑CGCCGCACATCCTCTg > 1:63727/1‑76 (MQ=255) aTCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCCCCAATCGTCtcc > 1:1423033/1‑74 (MQ=255) tctgttctgtTTT‑CGCGAGGTTTTGCGTCGCCACGATACTCTCCCACGGGCTCCCCCC‑TGCCGCAATTCGTCTGCa < 2:927760/76‑1 (MQ=255) tctggtctggTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCCCCCATCGTCTggc > 1:2019691/1‑74 (MQ=255) ctggtctggtTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGGGCC‑TCCCGCCCTTCGTCTGCAc > 2:5270635/1‑76 (MQ=255) ctggtctggtTC‑CGCCAGGTTTGCCGTCTGCACAATAACCCCCCCCTGGCTCCCCCC‑TGCCGCAATTCGTCTGCAc < 1:1964638/76‑1 (MQ=255) tgttctgttGT‑CGCCAGGATTACCGTCTGCAAGATAATCTCCCCCGGGCTGCCCCC‑TGCCGCAATTCGTCTGCAcg < 2:3665459/73‑1 (MQ=255) ggtctggtTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑TGCCGCCATTCGTCTGCAcgc > 1:2371675/1‑76 (MQ=255) ggtctggtTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGCGCCC‑CCGCGCCACTGGTCTGCAcgc > 1:3027314/1‑76 (MQ=255) gtctgttTC‑CGCCAGTTTTTACGTCCCCACGATAATCCCGCCCTGGCTGCCGCC‑TGCCGCAATTTGTCTGCAcgcg < 1:6495534/76‑1 (MQ=255) gtctggtTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGGGCC‑CGCCGCCCTTCTTCCTCAcgcg > 2:2891536/1‑76 (MQ=255) ctggtTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCGCCAATTGTCTGCAcgcggg > 1:569529/1‑74 (MQ=255) tggtTTGCGCGAGTATTTGCGTCGGCACAATAATCCCGCGCGGGCTCCCGCC‑TGCCGCAATTCGTCTGCACGCGTg < 2:592008/76‑1 (MQ=255) tggtTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGGGCC‑CGCCGCCACTCGTCTTCCCACGTGc > 2:6672304/1‑76 (MQ=255) ggtTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGGGCC‑CGCCGCCATTAGTCTTCCCCCGTggg > 2:1576451/1‑74 (MQ=255) gtTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCCCCATTCGTCTGCACGCgtggtg > 1:6254595/1‑76 (MQ=255) ttt‑CGCCGGGTTTTCCGTCGGCACGATAATCCCGCCCTGGCGCCCCCC‑TGCCGCAATTCGTCTGCACGCGTGCTgg < 2:547809/76‑1 (MQ=255) ttt‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCGCCATTCCTCTGCACGCCCGCTgg > 1:420362/1‑76 (MQ=255) t‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGGGCC‑CGCCGCCACTTGCCTTCCCCCGGGCTGGCg > 2:1383929/1‑76 (MQ=255) cGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCGCAAATCGTCCGCACGCGCGCCGGCgg > 1:2776453/1‑75 (MQ=255) gCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCCCCATTCGTCTGCACGCGGGCTGGCGtt > 2:5183120/1‑76 (MQ=255) ccAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑TGCCCCAATTCGTCTGCACGCGTGCTGGCGttt > 1:6416936/1‑75 (MQ=255) cAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCGCCAATCGTCTGCCCGCGCGCTGGCGGtat > 2:3214481/1‑76 (MQ=255) aGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑TGCCGCCATTCGTCTTCACGCGTGCTGGCGGtata > 1:1835660/1‑76 (MQ=255) aGGATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCGCCATTCGTCTGCACGCGGGCTGGCGGtatt > 1:5771059/1‑75 (MQ=255) gATTTACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCGCCATTCGTCTGCACCCGTGGCGGCGGtttata > 1:5549715/1‑76 (MQ=255) tttACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGGCCCCATTCGTCTGTACACGGGGTGGCGGTGTTTATc > 1:5654214/1‑76 (MQ=255) tttACGTCGCCCCGATACTCCGGCGCTGGCCGCCCCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATc < 2:62696/76‑1 (MQ=255) ttACGTCTGCACGATAATCTCGCCCTGGCTGGGGCC‑TGCCCCAATTCATCTGCACGCGCGTTGGCGTTATATATCg > 2:6793316/1‑76 (MQ=255) ttACGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCGCAAATCGTCTGCCCCCGGGCGGGCGGTatatattc > 1:3156607/1‑74 (MQ=255) gcctCTGCAAGATACCCTCCCGCGGCCCGCCGCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCt < 1:6642888/73‑1 (MQ=255) ccGTCGGCAGGATACTCTCGCACGGGCTCCCGCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCt < 2:4210526/75‑1 (MQ=255) cGTCTGCACGATAATCTCGCACTGGCTGGCGCC‑CGCCCCAAATCGTCCGCACCCGTGCCGGCGGTATATATCGCTa > 1:1957616/1‑76 (MQ=255) gTCTGCACGATAATCTCGCACTGGCTGGCGCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGGGGTATATATTGCtcc > 1:1431049/1‑74 (MQ=255) tccACGATACTCTCCCCCTGGCCCCCCCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTAcacc < 1:5747765/74‑1 (MQ=255) gCACGATAATCTCGCACTGGCTGCCGCC‑CGCCGCAAATCGTCTGCACGCGGGCTGGCGGTATATATTTCTAcacca > 1:5373357/1‑76 (MQ=255) cGATAATCTCGCACTGGCTGGCGCC‑TGCCCCAATTCGTCTGCCCCCGGGCTGGGGTTTTATATCGCTAAACCACGc > 1:3305211/1‑76 (MQ=255) cGATAATCTCGCACTGGCTGGCGCC‑CGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATTGCTACACCACGc > 1:5652461/1‑76 (MQ=255) aTAGTCGCGCGCTGGCTGCCCCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCa < 1:3681137/76‑1 (MQ=255) aTAATCTCGCACTGGCTGGGGCC‑CGCCGCCAATCGTCTGCACGCGCGCTGGCGTTATATATAGCTACACCAccccc > 2:6722013/1‑75 (MQ=255) tactctcGCCCGGGCTGCCCCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAg < 2:3338928/73‑1 (MQ=255) aaTCTCGCACTGGCTGGCGCCGTCCCCCAATT‑GTCCGCCCGCCGGCCGGGGTTATATATCTCTAACCCACCCCCGt > 2:6878473/1‑76 (MQ=255) aaTCTCGCACTGGCTGGCGCC‑CGCCCCCATTCCTCTGCCCGCCCGCCGGCCTTTTATATCGCTCCACCACGcccgc > 2:4425/1‑73 (MQ=255) ccACGGGCTCCCCCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGa < 2:4377546/75‑1 (MQ=255) tGGCTGCCGCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGGCGCa > 1:3745587/1‑76 (MQ=255) ctcctgccccc‑tgccgcAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCa < 1:4425436/73‑1 (MQ=255) ggCTGCCGCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCAt > 1:5323469/1‑76 (MQ=255) ggCTGCCGCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCAt > 2:4023774/1‑76 (MQ=255) ctgccgcc‑tgccgcAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATaa > 2:2706796/1‑76 (MQ=255) ctgccgcc‑tgccgcAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATaa > 1:42203/1‑76 (MQ=255) tgccgcc‑tgccgcAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATAAc > 2:3773337/1‑76 (MQ=255) tgccgcc‑tgccgcAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATAAc > 1:5183215/1‑76 (MQ=255) tgccgcc‑tgccgcAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATAAc > 1:6378753/1‑76 (MQ=255) tgccacc‑tgccgcAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATAAc > 1:4748944/1‑76 (MQ=255) cgccccc‑tgccgcAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATAAc < 1:5912811/75‑1 (MQ=255) ccccgcc‑tgccgcAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATAAc < 2:4590405/74‑1 (MQ=255) gccgcc‑tgccgcAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGGCGCATAACa > 1:496103/1‑76 (MQ=255) cccgcc‑tgccgcAATTCGTCTGCACGCGTGCCGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATAACa < 2:2873160/75‑1 (MQ=255) ccgcc‑tgccgcAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATAACAt > 2:4929728/1‑76 (MQ=255) | TCAGGAGTTTTATGATTACCCATCTGGTCTGGTTT‑CGCCAGGATTTACGTCTGCACGATAATCTCGCACTGGCTGCCGCC‑TGCCGCAATTCGTCTGCACGCGTGCTGGCGTTATATATCGCTACACCACGCCAGTGGGCGACGCATAACAT > REL606/720389‑720539 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |